ELISA/assay

NGF Rapid ELISA Kit: Human (1 Plate)

BEK-2212-1P

1 Plate Kit (1 x 96

NGF Rapid ELISA Kit: Human (1 Plate)

1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)1 Plate Kit (1 x 96 well plate)

The Biosensis NGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of human NGF in less than 3 hours. This kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3€™,5,5€™-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards. This NGF ELISA kit is designed to measure human NGF and thus employs a recombinant human NGF standard approved by the World Health Organization (WHO). Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including mouse and rat! The purpose of this kit is the in vitro quantitative determination of NGF in samples such as serum, plasma, cell culture supernatants and brain extracts. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore can recognize both the pro- and the mature form of NGF. However, human proNGF protein shows <0.1% cross-reactivity (determined at 25 ng/mL) allowing specific quantification of mature NGF.This NGF ELISA does not cross-react with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including mouse and rat!

Beta-nerve growth factor; Ngfb

FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.

Human

Foam box with ice packs

Store at 4°C

1288