ELISA/assay

BDNF Rapid ELISA Kit: Human, Mouse, Rat (2 plates)

BEK-2211-2P

2 Plate Kit (2 x 96

BDNF Rapid ELISA Kit: Human, Mouse, Rat (2 plates)

2 Plate Kit (2 x 96 well plate)

The Biosensis Rapid TM BDNF enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of BDNF in less than 3 hours with a simplified protocol and no loss of sensitivity or specificity. This kit consists of a pre-coated mouse monoclonal anti-BDNF capture antibody, a biotinylated anti-BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3€™,5,5€™-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of BDNF present in samples and protein standards. This BDNF ELISA kit employs a recombinant human BDNF standard approved by the World Health Organization (WHO, www.nibsc.org). The amino acid sequence of BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure BDNF in all these species and uses the same antibodies and antigen. The purpose of this kit is the in vitro quantitative determination of BDNF in samples such as serum, plasma (EDTA and citrate), cell culture supernatants and brain extracts. For measurement of BDNF in CSF samples, please contact us at sales@biosensis.com . This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

No cross-reactivity is observed for nerve growth factor (NGF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL.The reactivity of proBDNF full-length protein was determined and found to be less than 7%.

Brain-derived neurotrophic factor; BDNF; Abrineurin

BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's Disease, and Parkinson's disease. FUNCTION: BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. BDNF is also a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) . DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.

Human, mouse, rat and numerous other species.

Foam box with ice packs

Store at 4°C

1222