PIN-Cocktail 2 --- P504S / p63 / HMW Cytokeratin | BioLogo PIN002-G product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLogo

product type :

ELISA/assay

product name :

PIN-Cocktail 2 --- P504S / p63 / HMW Cytokeratin

catalog :

PIN002-G

quantity :

5 ml

price :

240 USD

product information

Catalog Number :

PIN002-G

Product Name :

PIN-Cocktail 2 --- P504S / p63 / HMW Cytokeratin

Host Species :

Rabbit/Mouse

Product Type :

Primary Antibodies

Size :

5 ml

List Price :

240 USD

Clone :

13H4 / 4A4 / 34ßE12

Isotype :

IgG / IgG2a / IgG1

CrossReactivity :

Human

Background :

P504S (AMACR, Alpha-methylacyl-CoA racemase) is an essential enzyme in the b-oxidation of branched-chain fatty acids. High expression of AMACR protein is found in prostate adenocarcinoma but not in benign prostate tissue by immunohistochemical staining in paraffin-embedded tissue. The expression of AMACR is also detected in prostate premalignant lesions, such as prostate intraepithelial neoplasia (PIN). The p63 protein, a homologue of the tumor-suppressor p53, is highly expressed in the nuclei of basal or progenitor layer of many epithelial tissues. p63 is detected in prostate basal cells in normal prostate glands and PIN. Other markers for basal cells are high molecular cytokeratins (CK 1, 5, 10 and 14), which can be labeled with HMW CK clone 34ßE12. In prostate adenocarcinoma basal cells disappear and staining with both p63 and HMW CK fails. Thus the combination of p63 and HMW CK is a useful tool as differential markers for benign prostate glands and adenocarcinoma (negative marker).

Immunogen :

1. Human recombinant AMACR (P504S) protein, whole sequence (for rabbit monoclonal antibody 13H4); 2. Recombinant human p63 protein aa 1-205 (for mouse monoclonal antibody 4A4); 3. Cytokeratin extract of human stratum corneum (for mouse monoclonal antibody 34ßE12)

Specificity :

1. Human AMACR (P504S) protein, Alpha-Methylacyl CoA Racemase, 2. human p63 nuclear protein, and 3. high molecular weight cytokeratins (56-57 and 66-68 kDa)

Application Summary :

IHC(C, P)

Storage :

2-8°C

General References :

1. Jiang Z, Woda BA, Rock KL et al. (2001) P504S: a new molecular marker for the detection of prostate carcinoma. Am J Surg Pathol 25:1397-1404.

Product References :

2. Weinstein MH, Signoretti S, Loda M (2002) Diagnostic utility of immunohistochemical staining for p63, a sensitive marker of prostatic basal cells. Mod Pathol. Dec;15(12):1302-8.