Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

Purified anti-ERG

940202

100 μg

293 USD

monoclonal

rat

nonconjugated

W19256A

western blot, immunocytochemistry, immunoprecipitation

WB, IP, ICC, ICFC

940202

W19256A

Purified anti-ERG

Rat IgG2a, κ

ETS-Related Gene, Transcriptional Regulator ERG, ETS Transcription Factor ERG, Erythroblast Transformation-Specific Transcription Factor ERG Variant 10, p55

100 μg

293 USD

Human

Monoclonal

Rat

PURE

Partial recombinant human ERG protein

This clone was tested for ICC using HeLa (low expressorl) and MOLT-4 (high expressor) cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Both methods resulted in robust ERG staining. This clone does not recognize mouse ER.When using this clone for ICFC, we recommend using the True-Nuclear™ Transcription Factor Buffer Set or the True Phos perm buffer. We do not recommend using the Intracellular Staining Permeabilization Wash Buffer for ICFC testing due to poor ERG staining.True-Nuclear™ Transcription Factor Buffer Set gave a better signal to noise ratio at all tested concentrations when compared to the True Phos perm buffer.;