Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

Purified anti-GATA1

939102

100 μg

335 USD

monoclonal

mouse

nonconjugated

P84F5

western blot, immunocytochemistry, immunoprecipitation

WB, ICC, IP, ICFC

939102

P84F5

Purified anti-GATA1

Mouse IgG2b, κ

GATA Binding Protein 1, Erythroid Transcription Factor, Globin Transcription Factor 1, NFE1, XLTT

100 μg

335 USD

Human

Monoclonal

Mouse

PURE

Full-length recombinant human GATA1 protein

This clone was tested for ICC using 4% PFA-fixed HEL cells permeabilized with either methanol or Triton X-100. While both methods were compatible with robust HEL staining, Triton X-100 permabilization resulted in stronger staining.When using this clone for ICFC, we recommend using the True-Nuclear™ Transcription Factor Buffer Set or the True Phos perm buffer. We do not recommend using the Intracellular Staining Permeabilization Wash Buffer for ICFC testing due to poor GATA1 staining.True-Nuclear™ Transcription Factor Buffer Set allowed for better binding to the epitope when compared to the True Phos perm buffer, there was a twofold difference between their median PE readings.;