Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

Purified anti-Vinculin

938401

25 μg

101 USD

monoclonal

rat

nonconjugated

W18245A

human, mouse

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

WB, ICC, IP, IHC-P

938401

W18245A

Purified anti-Vinculin

Rat IgG2a, κ

Metavinculin, MV, Epididymis Secretory Sperm Binding Protein, Epididymis Luminal Protein 114

25 μg

101 USD

Human, Mouse

Monoclonal

Rat

PURE

Partial recombinant human Vinculin protein

This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with Triton X-100 or methanol. While methanol permeabilization was compatible with Vinculin staining, Triton X-100 permeabilization resulted in dim staining. We therefore do not recommend Triton X-100 as a permeabilization method.While this clone passed IP testing, it failed to quantitatively enrich Vinculin from whole cell extracts and may not be suitable for detecting transient or low-abundance protein-protein interactions by co-immunoprecipitation.This clone is predicted to recognize Metavinculin due to complete sequence homology between the immunogen and the corresponding region of Metavinculin.;