Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

Purified anti-Lck Phospho (Tyr394)

933102

100 μg

265 USD

monoclonal

mouse

nonconjugated

A18002D

human, mouse

western blot, immunocytochemistry

WB, ICC, ICFC

933102

A18002D

Purified anti-Lck Phospho (Tyr394)

Mouse IgG1, κ

Lsk, p56lck, LCK Proto-Oncogene, Src Family Tyrosine Kinase, Tyrosine-protein kinase LCK, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, T cell-specific protein-tyrosine kinase

100 μg

265 USD

Human, Mouse

Monoclonal

Mouse

PURE

Synthetic peptide corresponding to human Src phosphorylated at tyrosine 419

This clone has not been tested for IP.This clone may recognize other Src family members.This clone was ICC tested on PFA-fixed cells using both methanol and Triton X-100 permeabilization methods. Both permeabilazation methods were compatible with staining. Triton X-100 permeabilization produces stronger staining.During ICC product development testing, this clone produced strong nuclear staining in a small subpopulation (<1%) of both unstimulated and stimulated cells. The source of this staining is not clear but we do not believe it is Lck-dependent.This clone was developed using a synthetic peptide corresponding to human Src phosphorylated at tyrosine 419, which displays high sequence homology with the region surrounding Lck tyrosine 394. To confirm specificity to Lck Phospho (Tyr394), the cell line J.Cam1.6 was used. This cell line is a clonal derivative of Jurkat cells that harbors a truncated LCK allele with defective protein expression (Straus, et al. 1992. Cell. 70:585).;