Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

Direct-Blot™ HRP anti-RNA Polymerase II RPB1

920305

25 μL

112 USD

monoclonal

mouse

nonconjugated

H14

human, mouse, rat

WB

920305

H14

Direct-Blot™ HRP anti-RNA Polymerase II RPB1

Mouse IgM, κ

DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, RNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit

25 μL

112 USD

Human, Mouse, Rat

Monoclonal

Mouse

DB_HRP

Additional reported applications (for the relevant formats) include: immunoprecipitation^2,3.The clone H14 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites: ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. Clone H14 recognizes the phosphoserine 5 (ser-5) of Pol II. Clone H14 is useful for immunofluorescence and Western blotting.This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).;