Purified anti-RNA Polymerase II RPB1 | BioLegend 920304 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-RNA Polymerase II RPB1

catalog :

920304

quantity :

100 μg

price :

323 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

H14

reactivity :

fission yeast, human, mouse, rat, brewer's yeast, fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

BioLegend product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:50; loading ...; fig 2d	Nagaraj R, Sharpley M, Chi F, Braas D, Zhou Y, Kim R, et al. Nuclear Localization of Mitochondrial TCA Cycle Enzymes as a Critical Step in Mammalian Zygotic Genome Activation. Cell. 2017;168:210-223.e11 pubmed publisher
western blot; brewer's yeast; fig 3C	Sathianathan A, Ravichandran P, Lippi J, Cohen L, Messina A, Shaju S, et al. The Eaf3/5/7 Subcomplex Stimulates NuA4 Interaction with Methylated Histone H3 Lys-36 and RNA Polymerase II. J Biol Chem. 2016;291:21195-21207 pubmed
western blot; fission yeast; loading ...; fig 2a	RodrÃguez Molina J, Tseng S, Simonett S, Taunton J, Ansari A. Engineered Covalent Inactivation of TFIIH-Kinase Reveals an Elongation Checkpoint and Results in Widespread mRNA Stabilization. Mol Cell. 2016;63:433-44 pubmed publisher
chromatin immunoprecipitation; fruit fly ; fig s3b	Sadasivam D, Huang D. Maintenance of Tissue Pluripotency by Epigenetic Factors Acting at Multiple Levels. PLoS Genet. 2016;12:e1005897 pubmed publisher
western blot; human; fig 2	Liang K, Gao X, Gilmore J, Florens L, Washburn M, Smith E, et al. Characterization of human cyclin-dependent kinase 12 (CDK12) and CDK13 complexes in C-terminal domain phosphorylation, gene transcription, and RNA processing. Mol Cell Biol. 2015;35:928-38 pubmed publisher
	Bharath L, Agrawal M, McCambridge G, Nicholas D, Hasturk H, Liu J, et al. Metformin Enhances Autophagy and Normalizes Mitochondrial Function to Alleviate Aging-Associated Inflammation. Cell Metab. 2020;: pubmed publisher
	Tolza C, Bejjani F, Evanno E, Mahfoud S, Moquet Torcy G, Gostan T, et al. AP-1 Signaling by Fra-1 Directly Regulates HMGA1 Oncogene Transcription in Triple-Negative Breast Cancers. Mol Cancer Res. 2019;17:1999-2014 pubmed publisher

product information

Antigen :

RNA Polymerase II RPB1

Apps. Abbrev. :

WB, ICC, IP

Cat # :

920304

Clone :

H14

Item :

Purified anti-RNA Polymerase II RPB1

Isotype :

Mouse IgM, κ

Other Names :

DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, RNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit

Size :

100 μg

Price (USD) :

323 USD

Reactivity :

Human, Mouse, Rat

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PURE

Application Notes :

Additional reported applications (for the relevant formats) include: immunoprecipitation^2,3.The clone H14 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites: ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. Clone H14 recognizes the phosphoserine 5 (ser-5) of Pol II. Clone H14 is useful for immunofluorescence and Western blotting.This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).;

more info or order :

BioLegend product webpage