Purified anti-RNA Polymerase II RPB1 | BioLegend 920203 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-RNA Polymerase II RPB1

catalog :

920203

quantity :

25 μg

price :

89 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human, rat

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

BioLegend product webpage

citations: 15

Reference
Chappidi N, Nascakova Z, Boleslavska B, Zellweger R, Isik E, Andrs M, et al. Fork Cleavage-Religation Cycle and Active Transcription Mediate Replication Restart after Fork Stalling at Co-transcriptional R-Loops. Mol Cell. 2019;: pubmed publisher
Zhou K, Shi H, Lyu R, Wylder A, Matuszek Ż, Pan J, et al. Regulation of Co-transcriptional Pre-mRNA Splicing by m6A through the Low-Complexity Protein hnRNPG. Mol Cell. 2019;76:70-81.e9 pubmed publisher
Kuhn T, Pascual García P, Gozalo A, Little S, Capelson M. Chromatin targeting of nuclear pore proteins induces chromatin decondensation. J Cell Biol. 2019;218:2945-2961 pubmed publisher
Ryu H, Su D, Wilson Eisele N, Zhao D, Lopez Giraldez F, Hochstrasser M. The Ulp2 SUMO protease promotes transcription elongation through regulation of histone sumoylation. EMBO J. 2019;38:e102003 pubmed publisher
Tolza C, Bejjani F, Evanno E, Mahfoud S, Moquet Torcy G, Gostan T, et al. AP-1 Signaling by Fra-1 Directly Regulates HMGA1 Oncogene Transcription in Triple-Negative Breast Cancers. Mol Cancer Res. 2019;17:1999-2014 pubmed publisher
Baluapuri A, Hofstetter J, Dudvarski Stankovic N, Endres T, Bhandare P, Vos S, et al. MYC Recruits SPT5 to RNA Polymerase II to Promote Processive Transcription Elongation. Mol Cell. 2019;74:674-687.e11 pubmed publisher
Zhang H, Pandey S, Travers M, Sun H, Morton G, Madzo J, et al. Targeting CDK9 Reactivates Epigenetically Silenced Genes in Cancer. Cell. 2018;175:1244-1258.e26 pubmed publisher
Wong M, Belew M, Kwieraga A, Nhan J, Michael W. Programmed DNA Breaks Activate the Germline Genome in Caenorhabditis elegans. Dev Cell. 2018;46:302-315.e5 pubmed publisher
Róna G, Roberti D, Yin Y, Pagan J, Homer H, Sassani E, et al. PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading. elife. 2018;7: pubmed publisher
Fitzwalter B, Towers C, Sullivan K, Andrysik Z, Hoh M, Ludwig M, et al. Autophagy Inhibition Mediates Apoptosis Sensitization in Cancer Therapy by Relieving FOXO3a Turnover. Dev Cell. 2018;44:555-565.e3 pubmed publisher
Yurko N, Liu X, Yamazaki T, Hoque M, Tian B, Manley J. MPK1/SLT2 Links Multiple Stress Responses with Gene Expression in Budding Yeast by Phosphorylating Tyr1 of the RNAP II CTD. Mol Cell. 2017;68:913-925.e3 pubmed publisher
Butler A, Owens D, Wang L, King M. A novel role for sox7 in Xenopus early primordial germ cell development: mining the PGC transcriptome. Development. 2018;145: pubmed publisher
Li Y, Wang C, Cai W, Sengupta S, Zavortink M, Deng H, et al. H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation. Development. 2017;144:3232-3240 pubmed publisher
Yamashita T, Aizawa H, Teramoto S, Akamatsu M, Kwak S. Calpain-dependent disruption of nucleo-cytoplasmic transport in ALS motor neurons. Sci Rep. 2017;7:39994 pubmed publisher
Oqani R, Lin T, Lee J, Choi K, Shin H, Jin D. P-TEFb Kinase Activity Is Essential for Global Transcription, Resumption of Meiosis and Embryonic Genome Activation in Pig. PLoS ONE. 2016;11:e0152254 pubmed publisher

product information

Antigen :

RNA Polymerase II

Apps. Abbrev. :

WB, ICC, IP

Cat # :

920203

Clone :

Item :

Purified anti-RNA Polymerase II RPB1

Isotype :

Mouse IgM, κ

Other Names :

DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, RNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit

Size :

25 μg

Price (USD) :

89 USD

Reactivity :

Human, Rat

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PURE

Immunogen :

The antibody H5 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line.

Application Notes :

Additional reported applications (for the relevant formats) include: immunoprecipitation^2,3.The clone H5 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites: ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. Clone H5 recognizes the phosphoserine 2 (ser-2) of Pol II. Clone H5 is useful for immunofluorescence and Western blotting.This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).This product weakly reacts with Mouse.;

more info or order :

BioLegend product webpage