product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified Non-muscle Myosin Heavy Chain II-B
catalog :
909901
quantity :
100 μL
price :
288 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
Poly19099
reactivity :
human, mouse, rat, dogs
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 30
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 7b
Naydenov N, Lechuga S, Zalavadia A, Mukherjee P, Gordon I, Skvasik D, et al. P-Cadherin Regulates Intestinal Epithelial Cell Migration and Mucosal Repair, but Is Dispensable for Colitis Associated Colon Cancer. Cells. 2022;11: pubmed publisher
  • immunocytochemistry; dogs; fig 2
Tokuda S, Hirai T, Furuse M. Claudin-4 knockout by TALEN-mediated gene targeting in MDCK cells: Claudin-4 is dispensable for the permeability properties of tight junctions in wild-type MDCK cells. PLoS ONE. 2017;12:e0182521 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5d
Priya R, Liang X, Teo J, Duszyc K, Yap A, Gomez G. ROCK1 but not ROCK2 contributes to RhoA signaling and NMIIA-mediated contractility at the epithelial zonula adherens. Mol Biol Cell. 2017;28:12-20 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 1e
  • western blot; human; loading ...; fig 1f
Priya R, Wee K, Budnar S, Gomez G, Yap A, Michael M. Coronin 1B supports RhoA signaling at cell-cell junctions through Myosin II. Cell Cycle. 2016;15:3033-3041 pubmed
  • immunohistochemistry; mouse; 1:50; fig 5
Grego Bessa J, Bloomekatz J, Castel P, Omelchenko T, Baselga J, Anderson K. The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium. elife. 2016;5:e12034 pubmed publisher
  • immunohistochemistry; mouse; 1:1000
Loebel D, Plageman T, Tang T, Jones V, Muccioli M, Tam P. Thyroid bud morphogenesis requires CDC42- and SHROOM3-dependent apical constriction. Biol Open. 2016;5:130-9 pubmed publisher
Weißenbruch K, Grewe J, Hippler M, Fladung M, Tremmel M, Stricker K, et al. Distinct roles of nonmuscle myosin II isoforms for establishing tension and elasticity during cell morphodynamics. elife. 2021;10: pubmed publisher
Ning W, Muroyama A, Li H, Lechler T. Differentiated Daughter Cells Regulate Stem Cell Proliferation and Fate through Intra-tissue Tension. Cell Stem Cell. 2021;28:436-452.e5 pubmed publisher
Belardi B, Hamkins Indik T, Harris A, Kim J, Xu K, Fletcher D. A Weak Link with Actin Organizes Tight Junctions to Control Epithelial Permeability. Dev Cell. 2020;54:792-804.e7 pubmed publisher
Aguilar Cuenca R, Llorente Gonzalez C, Chapman J, Talayero V, Garrido Casado M, Delgado Arevalo C, et al. Tyrosine Phosphorylation of the Myosin Regulatory Light Chain Controls Non-muscle Myosin II Assembly and Function in Migrating Cells. Curr Biol. 2020;30:2446-2458.e6 pubmed publisher
Wang X, Yang S, Zhang C, Hu M, Qian J, Ma J, et al. Knocking Out Non-muscle Myosin II in Retinal Ganglion Cells Promotes Long-Distance Optic Nerve Regeneration. Cell Rep. 2020;31:107537 pubmed publisher
Yamada S, Lav R, Li J, Tucker A, Green J. Molar Bud-to-Cap Transition Is Proliferation Independent. J Dent Res. 2019;98:1253-1261 pubmed publisher
Zhovmer A, Tabdanov E, Miao H, Wen H, Chen J, Luo X, et al. The role of nonmuscle myosin 2A and 2B in the regulation of mesenchymal cell contact guidance. Mol Biol Cell. 2019;30:1961-1973 pubmed publisher
Awadia S, Huq F, Arnold T, Goicoechea S, Sun Y, Hou T, et al. SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility. J Cell Biol. 2019;: pubmed publisher
Butler M, Short N, Maniou E, Alexandre P, Greene N, Copp A, et al. Rho kinase-dependent apical constriction counteracts M-phase apical expansion to enable mouse neural tube closure. J Cell Sci. 2019;: pubmed publisher
Eaton A, Clayton D, Ruiz W, Griffiths S, RUBIO M, Apodaca G. Expansion and Contraction of the Umbrella Cell Apical Junctional Ring in Response to Bladder Filling and Voiding. Mol Biol Cell. 2019;:mbcE19020115 pubmed publisher
Jiu Y, Kumari R, Fenix A, Schaible N, Liu X, Varjosalo M, et al. Myosin-18B Promotes the Assembly of Myosin II Stacks for Maturation of Contractile Actomyosin Bundles. Curr Biol. 2019;29:81-92.e5 pubmed publisher
Fenix A, Neininger A, Taneja N, Hyde K, Visetsouk M, Garde R, et al. Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes. elife. 2018;7: pubmed publisher
Sumigray K, Terwilliger M, Lechler T. Morphogenesis and Compartmentalization of the Intestinal Crypt. Dev Cell. 2018;45:183-197.e5 pubmed publisher
Chopra A, Kutys M, Zhang K, Polacheck W, Sheng C, Luu R, et al. Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions. Dev Cell. 2018;44:87-96.e5 pubmed publisher
Van Itallie C, Tietgens A, Aponte A, Gucek M, Cartagena Rivera A, Chadwick R, et al. MARCKS-related protein regulates cytoskeletal organization at cell-cell and cell-substrate contacts in epithelial cells. J Cell Sci. 2018;131: pubmed publisher
Otterpohl K, Hart R, Evans C, Surendran K, Chandrasekar I. Nonmuscle myosin 2 proteins encoded by Myh9, Myh10, and Myh14 are uniquely distributed in the tubular segments of murine kidney. Physiol Rep. 2017;5: pubmed publisher
Lehtimäki J, Fenix A, Kotila T, Balistreri G, Paavolainen L, Varjosalo M, et al. UNC-45a promotes myosin folding and stress fiber assembly. J Cell Biol. 2017;216:4053-4072 pubmed publisher
Cartagena Rivera A, Van Itallie C, Anderson J, Chadwick R. Apical surface supracellular mechanical properties in polarized epithelium using noninvasive acoustic force spectroscopy. Nat Commun. 2017;8:1030 pubmed publisher
Nguyen Ngoc K, Silvestri V, Georgess D, Fairchild A, Ewald A. Mosaic loss of non-muscle myosin IIA and IIB is sufficient to induce mammary epithelial proliferation. J Cell Sci. 2017;130:3213-3221 pubmed publisher
Trivedi N, Stabley D, Cain B, Howell D, Laumonnerie C, Ramahi J, et al. Drebrin-mediated microtubule-actomyosin coupling steers cerebellar granule neuron nucleokinesis and migration pathway selection. Nat Commun. 2017;8:14484 pubmed publisher
Salomon J, Gaston C, Magescas J, Duvauchelle B, Canioni D, Sengmanivong L, et al. Contractile forces at tricellular contacts modulate epithelial organization and monolayer integrity. Nat Commun. 2017;8:13998 pubmed publisher
Ramkumar N, Omelchenko T, Silva Gagliardi N, McGlade C, Wijnholds J, Anderson K. Crumbs2 promotes cell ingression during the epithelial-to-mesenchymal transition at gastrulation. Nat Cell Biol. 2016;18:1281-1291 pubmed publisher
Tokuda S, Hirai T, Furuse M. Effects of Osmolality on Paracellular Transport in MDCK II Cells. PLoS ONE. 2016;11:e0166904 pubmed publisher
Li X, Maretzky T, Weskamp G, Monette S, Qing X, Issuree P, et al. iRhoms 1 and 2 are essential upstream regulators of ADAM17-dependent EGFR signaling. Proc Natl Acad Sci U S A. 2015;112:6080-5 pubmed publisher
product information
Antigen :
Myosin-10
Apps. Abbrev. :
WB, ICC, IHC-P, IHC-F
Cat # :
909901
Clone :
Poly19099
Item :
Purified Non-muscle Myosin Heavy Chain II-B
Isotype :
Rabbit Polyclonal IgG
Other Names :
Myosin-10, NMMHC-B, myosin heavy chain 10, nonmuscle myosin heavy chain-B, nonmuscle myosin heavy chain IIB, cellular myosin heavy chain, type B type B
Size :
100 μL
Price (USD) :
288 USD
Reactivity :
Human, Mouse, Rat
Clonality :
Polyclonal
Host :
Rabbit
Conjugate/Tag/Label :
PURE
Immunogen :
This antibody was raised against the peptide SDVNETQPPQSE corresponding to the C-terminus of nonmuscle myosin heavy chain isoform B (MHC-B).
Application Notes :
This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC) and immunofluorescence (IF).*Predicted MW = 200 kDPositive Control: human smooth muscle;This product may contain other non-IgG subtypes.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA