product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified Non-muscle Myosin Heavy Chain II-A
catalog :
909801
quantity :
100 μL
price :
288 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
Poly19098
reactivity :
human, mouse, rat, dogs, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
citations: 51
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 7b
Naydenov N, Lechuga S, Zalavadia A, Mukherjee P, Gordon I, Skvasik D, et al. P-Cadherin Regulates Intestinal Epithelial Cell Migration and Mucosal Repair, but Is Dispensable for Colitis Associated Colon Cancer. Cells. 2022;11: pubmed publisher
  • immunocytochemistry; human; 1:800; loading ...; fig 1a
  • immunocytochemistry; mouse; 1:800; loading ...; fig 1a
Lehtimäki J, Rajakylä E, Tojkander S, Lappalainen P. Generation of stress fibers through myosin-driven reorganization of the actin cortex. elife. 2021;10: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 3g
Jiu Y, Kumari R, Fenix A, Schaible N, Liu X, Varjosalo M, et al. Myosin-18B Promotes the Assembly of Myosin II Stacks for Maturation of Contractile Actomyosin Bundles. Curr Biol. 2019;29:81-92.e5 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig s1a
Ciuba K, Hawkes W, Tojkander S, Kogan K, Engel U, Iskratsch T, et al. Calponin-3 is critical for coordinated contractility of actin stress fibers. Sci Rep. 2018;8:17670 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig s5j
Schell C, Sabass B, Helmstaedter M, Geist F, Abed A, Yasuda Yamahara M, et al. ARP3 Controls the Podocyte Architecture at the Kidney Filtration Barrier. Dev Cell. 2018;47:741-757.e8 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 5d, 7b
  • western blot; human; 1:1000; loading ...; fig 5b
Latham S, Ehmke N, Reinke P, Taft M, Eicke D, Reindl T, et al. Variants in exons 5 and 6 of ACTB cause syndromic thrombocytopenia. Nat Commun. 2018;9:4250 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 6c
Yasuda Yamahara M, Rogg M, Yamahara K, Maier J, Huber T, Schell C. AIF1L regulates actomyosin contractility and filopodial extensions in human podocytes. PLoS ONE. 2018;13:e0200487 pubmed publisher
  • immunocytochemistry; dogs; loading ...
Fredriksson Lidman K, Van Itallie C, Tietgens A, Anderson J. Sorbin and SH3 domain-containing protein 2 (SORBS2) is a component of the acto-myosin ring at the apical junctional complex in epithelial cells. PLoS ONE. 2017;12:e0185448 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s8a
Owen L, Adhikari A, Patel M, Grimmer P, Leijnse N, Kim M, et al. A cytoskeletal clutch mediates cellular force transmission in a soft, three-dimensional extracellular matrix. Mol Biol Cell. 2017;28:1959-1974 pubmed publisher
  • western blot; human; loading ...; fig s4a
Jeong S, Lim S, Schevzov G, Gunning P, Helfman D. Loss of Tpm4.1 leads to disruption of cell-cell adhesions and invasive behavior in breast epithelial cells via increased Rac1 signaling. Oncotarget. 2017;8:33544-33559 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5c
Priya R, Liang X, Teo J, Duszyc K, Yap A, Gomez G. ROCK1 but not ROCK2 contributes to RhoA signaling and NMIIA-mediated contractility at the epithelial zonula adherens. Mol Biol Cell. 2017;28:12-20 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 1d
  • western blot; human; loading ...; fig 1f
Priya R, Wee K, Budnar S, Gomez G, Yap A, Michael M. Coronin 1B supports RhoA signaling at cell-cell junctions through Myosin II. Cell Cycle. 2016;15:3033-3041 pubmed
  • immunocytochemistry; human; loading ...; fig 1a
Taneja N, Fenix A, Rathbun L, Millis B, Tyska M, Hehnly H, et al. Focal adhesions control cleavage furrow shape and spindle tilt during mitosis. Sci Rep. 2016;6:29846 pubmed publisher
  • immunohistochemistry; zebrafish ; 1:100; fig 4
Gebala V, Collins R, Geudens I, Phng L, Gerhardt H. Blood flow drives lumen formation by inverse membrane blebbing during angiogenesis in vivo. Nat Cell Biol. 2016;18:443-50 pubmed publisher
Weißenbruch K, Grewe J, Hippler M, Fladung M, Tremmel M, Stricker K, et al. Distinct roles of nonmuscle myosin II isoforms for establishing tension and elasticity during cell morphodynamics. elife. 2021;10: pubmed publisher
Taneja N, Baillargeon S, Burnette D. Myosin Light Chain Kinase driven myosin II turnover regulates actin cortex contractility during mitosis. Mol Biol Cell. 2021;:mbcE20090608 pubmed publisher
Gaston C, de Beco S, Doss B, Pan M, Gauquelin E, D Alessandro J, et al. EpCAM promotes endosomal modulation of the cortical RhoA zone for epithelial organization. Nat Commun. 2021;12:2226 pubmed publisher
Zaman R, Lombardo A, Sauvanet C, Viswanatha R, Awad V, Bonomo L, et al. Effector-mediated ERM activation locally inhibits RhoA activity to shape the apical cell domain. J Cell Biol. 2021;220: pubmed publisher
Ning W, Muroyama A, Li H, Lechler T. Differentiated Daughter Cells Regulate Stem Cell Proliferation and Fate through Intra-tissue Tension. Cell Stem Cell. 2021;28:436-452.e5 pubmed publisher
Aguilar Cuenca R, Llorente Gonzalez C, Chapman J, Talayero V, Garrido Casado M, Delgado Arevalo C, et al. Tyrosine Phosphorylation of the Myosin Regulatory Light Chain Controls Non-muscle Myosin II Assembly and Function in Migrating Cells. Curr Biol. 2020;30:2446-2458.e6 pubmed publisher
Taneja N, Bersi M, Baillargeon S, Fenix A, Cooper J, Ohi R, et al. Precise Tuning of Cortical Contractility Regulates Cell Shape during Cytokinesis. Cell Rep. 2020;31:107477 pubmed publisher
Rajakylä E, Lehtimäki J, Acheva A, Schaible N, Lappalainen P, Krishnan R, et al. Assembly of Peripheral Actomyosin Bundles in Epithelial Cells Is Dependent on the CaMKK2/AMPK Pathway. Cell Rep. 2020;30:4266-4280.e4 pubmed publisher
Indra I, Troyanovsky R, Shapiro L, Honig B, Troyanovsky S. Sensing Actin Dynamics through Adherens Junctions. Cell Rep. 2020;30:2820-2833.e3 pubmed publisher
Kumari S, Mak M, Poh Y, Tohme M, Watson N, Melo M, et al. Cytoskeletal tension actively sustains the migratory T-cell synaptic contact. EMBO J. 2020;39:e102783 pubmed publisher
Zhovmer A, Tabdanov E, Miao H, Wen H, Chen J, Luo X, et al. The role of nonmuscle myosin 2A and 2B in the regulation of mesenchymal cell contact guidance. Mol Biol Cell. 2019;30:1961-1973 pubmed publisher
Eaton A, Clayton D, Ruiz W, Griffiths S, RUBIO M, Apodaca G. Expansion and Contraction of the Umbrella Cell Apical Junctional Ring in Response to Bladder Filling and Voiding. Mol Biol Cell. 2019;:mbcE19020115 pubmed publisher
Choi J, Troyanovsky R, Indra I, Mitchell B, Troyanovsky S. Scribble, Erbin, and Lano redundantly regulate epithelial polarity and apical adhesion complex. J Cell Biol. 2019;: pubmed publisher
Taneja N, Burnette D. Myosin IIA drives membrane bleb retraction. Mol Biol Cell. 2019;30:1051-1059 pubmed publisher
Schiffhauer E, Ren Y, Iglesias V, Kothari P, Iglesias P, Robinson D. Myosin IIB assembly state determines its mechanosensitive dynamics. J Cell Biol. 2019;218:895-908 pubmed publisher
Wang F, Chen S, Liu H, Parent C, Coulombe P. Keratin 6 regulates collective keratinocyte migration by altering cell-cell and cell-matrix adhesion. J Cell Biol. 2018;217:4314-4330 pubmed publisher
Cheng C, Nowak R, Amadeo M, Biswas S, Lo W, Fowler V. Tropomyosin 3.5 protects the F-actin networks required for tissue biomechanical properties. J Cell Sci. 2018;131: pubmed publisher
Smith T, Saul R, Barton E, Luna E. Generation and characterization of monoclonal antibodies that recognize human and murine supervillin protein isoforms. PLoS ONE. 2018;13:e0205910 pubmed publisher
Li J, Yuan Y, He J, Feng J, Han X, Jing J, et al. Constitutive activation of hedgehog signaling adversely affects epithelial cell fate during palatal fusion. Dev Biol. 2018;441:191-203 pubmed publisher
Sumigray K, Terwilliger M, Lechler T. Morphogenesis and Compartmentalization of the Intestinal Crypt. Dev Cell. 2018;45:183-197.e5 pubmed publisher
Masedunskas A, Appaduray M, Lucas C, Lastra Cagigas M, Heydecker M, Holliday M, et al. Parallel assembly of actin and tropomyosin, but not myosin II, during de novo actin filament formation in live mice. J Cell Sci. 2018;131: pubmed publisher
Chopra A, Kutys M, Zhang K, Polacheck W, Sheng C, Luu R, et al. Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions. Dev Cell. 2018;44:87-96.e5 pubmed publisher
Van Itallie C, Tietgens A, Aponte A, Gucek M, Cartagena Rivera A, Chadwick R, et al. MARCKS-related protein regulates cytoskeletal organization at cell-cell and cell-substrate contacts in epithelial cells. J Cell Sci. 2018;131: pubmed publisher
Otterpohl K, Hart R, Evans C, Surendran K, Chandrasekar I. Nonmuscle myosin 2 proteins encoded by Myh9, Myh10, and Myh14 are uniquely distributed in the tubular segments of murine kidney. Physiol Rep. 2017;5: pubmed publisher
Lehtimäki J, Fenix A, Kotila T, Balistreri G, Paavolainen L, Varjosalo M, et al. UNC-45a promotes myosin folding and stress fiber assembly. J Cell Biol. 2017;216:4053-4072 pubmed publisher
Cechova S, Dong F, Chan F, Kelley M, Ruiz P, Le T. MYH9 E1841K Mutation Augments Proteinuria and Podocyte Injury and Migration. J Am Soc Nephrol. 2018;29:155-167 pubmed publisher
Moreau J, Cen S, Berger A, Furlonger C, Paige C. Bone marrow basophils provide survival signals to immature B cells in vitro but are dispensable in vivo. PLoS ONE. 2017;12:e0185509 pubmed publisher
Nguyen Ngoc K, Silvestri V, Georgess D, Fairchild A, Ewald A. Mosaic loss of non-muscle myosin IIA and IIB is sufficient to induce mammary epithelial proliferation. J Cell Sci. 2017;130:3213-3221 pubmed publisher
Fukuda S, Matsui T, Ichikawa T, Furukawa T, Kioka N, Fukushima S, et al. Cellular force assay detects altered contractility caused by a nephritis-associated mutation in nonmuscle myosin IIA. Dev Growth Differ. 2017;59:423-433 pubmed publisher
Pfisterer S, Gateva G, Horvath P, Pirhonen J, Salo V, Karhinen L, et al. Role for formin-like 1-dependent acto-myosin assembly in lipid droplet dynamics and lipid storage. Nat Commun. 2017;8:14858 pubmed publisher
Salomon J, Gaston C, Magescas J, Duvauchelle B, Canioni D, Sengmanivong L, et al. Contractile forces at tricellular contacts modulate epithelial organization and monolayer integrity. Nat Commun. 2017;8:13998 pubmed publisher
Meddens M, Pandzic E, Slotman J, Guillet D, Joosten B, Mennens S, et al. Actomyosin-dependent dynamic spatial patterns of cytoskeletal components drive mesoscale podosome organization. Nat Commun. 2016;7:13127 pubmed publisher
Le H, Ghatak S, Yeung C, Tellkamp F, Günschmann C, Dieterich C, et al. Mechanical regulation of transcription controls Polycomb-mediated gene silencing during lineage commitment. Nat Cell Biol. 2016;18:864-75 pubmed publisher
Murakami K, Eguchi J, Hida K, Nakatsuka A, Katayama A, Sakurai M, et al. Antiobesity Action of ACAM by Modulating the Dynamics of Cell Adhesion and Actin Polymerization in Adipocytes. Diabetes. 2016;65:1255-67 pubmed publisher
Chen P, Jian X, Heissler S, Le K, Luo R, Jenkins L, et al. The Arf GTPase-activating Protein, ASAP1, Binds Nonmuscle Myosin 2A to Control Remodeling of the Actomyosin Network. J Biol Chem. 2016;291:7517-26 pubmed publisher
Makowska K, Hughes R, White K, Wells C, Peckham M. Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells. Cell Rep. 2015;13:2118-25 pubmed publisher
Yu H, Dohn M, Markham N, Coffey R, Reynolds A. p120-catenin controls contractility along the vertical axis of epithelial lateral membranes. J Cell Sci. 2016;129:80-94 pubmed publisher
product information
Antigen :
Myosin-9
Apps. Abbrev. :
Western Blotting, ICC
Cat # :
909801
Clone :
Poly19098
Item :
Purified Non-muscle Myosin Heavy Chain II-A
Isotype :
Rabbit Polyclonal IgG
Other Names :
Myosin-9, NMMHC-A, MYH9 variant protein, myosin heavy chain 9, non-muscle myosin heavy chain A, cellular myosin heavy chain, type A, non-muscle myosin heavy polypeptide 9
Size :
100 μL
Price (USD) :
288 USD
Reactivity :
Human, Mouse, Rat
Clonality :
Polyclonal
Host :
Rabbit
Conjugate/Tag/Label :
PURE
Immunogen :
This antibody was raised against the peptide GKADGAEAKPAE corresponding to the C-terminus of human nonmuscle myosin heavy chain isoform A (MHC-A, NMHC IIA, Myosin-9).
Application Notes :
This antibody is effective in immunoblotting (WB) and immunofluorescence (IF). *Predicted MW = 200 kD . This product may contain other non-IgG subtypes.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA