Direct-Blot™ HRP anti-Nuclear Pore Complex Proteins | BioLegend 902905 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Direct-Blot™ HRP anti-Nuclear Pore Complex Proteins

catalog :

902905

quantity :

100 μL

price :

340 USD

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

MAb414

reactivity :

Xenopus laevis

application :

western blot, immunohistochemistry

citations: 30

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; Xenopus laevis; 1:1000; loading ...; fig 1a	Edens L, Dilsaver M, Levy D. PKC-mediated phosphorylation of nuclear lamins at a single serine residue regulates interphase nuclear size in Xenopus and mammalian cells. Mol Biol Cell. 2017;28:1389-1399 pubmed publisher
immunohistochemistry; Xenopus laevis; 1:1000; loading ...; fig 3a	VukoviÄ‡ L, JevtiÄ‡ P, Zhang Z, Stohr B, Levy D. Nuclear size is sensitive to NTF2 protein levels in a manner dependent on Ran binding. J Cell Sci. 2016;129:1115-27 pubmed publisher
	Hollern D, Xu N, Thennavan A, Glodowski C, García Recio S, Mott K, et al. B Cells and T Follicular Helper Cells Mediate Response to Checkpoint Inhibitors in High Mutation Burden Mouse Models of Breast Cancer. Cell. 2019;179:1191-1206.e21 pubmed publisher
	Hampoelz B, Schwarz A, Ronchi P, Bragulat Teixidor H, Tischer C, Gaspar I, et al. Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis. Cell. 2019;179:671-686.e17 pubmed publisher
	Ahn J, Cho M, Sohn S, Lee J. Inhibition of PP2A activity by H2O2 during mitosis disrupts nuclear envelope reassembly and alters nuclear shape. Exp Mol Med. 2019;51:64 pubmed publisher
	Ponjavic A, Ye Y, Laue E, Lee S, Klenerman D. Sensitive light-sheet microscopy in multiwell plates using an AFM cantilever. Biomed Opt Express. 2018;9:5863-5880 pubmed publisher
	Biffi G, Oni T, Spielman B, Hao Y, Elyada E, Park Y, et al. IL1-Induced JAK/STAT Signaling Is Antagonized by TGFβ to Shape CAF Heterogeneity in Pancreatic Ductal Adenocarcinoma. Cancer Discov. 2019;9:282-301 pubmed publisher
	Dilsaver M, Chen P, Thompson T, Reusser T, Mukherjee R, Oakey J, et al. Emerin induces nuclear breakage in Xenopus extract and early embryos. Mol Biol Cell. 2018;:mbcE18050277 pubmed publisher
	Borlido J, Sakuma S, Raices M, Carrette F, Tinoco R, Bradley L, et al. Nuclear pore complex-mediated modulation of TCR signaling is required for naïve CD4+ T cell homeostasis. Nat Immunol. 2018;19:594-605 pubmed publisher
	Watters K, Inankur B, Gardiner J, Warrick J, Sherer N, Yin J, et al. Differential Disruption of Nucleocytoplasmic Trafficking Pathways by Rhinovirus 2A Proteases. J Virol. 2017;91: pubmed publisher
	Behrens R, Aligeti M, Pocock G, Higgins C, Sherer N. Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export. J Virol. 2017;91: pubmed publisher
	Zheng X, Yang S, Han Y, Zhao X, Zhao L, Tian T, et al. Loss of zygotic NUP107 protein causes missing of pharyngeal skeleton and other tissue defects with impaired nuclear pore function in zebrafish embryos. J Biol Chem. 2012;287:38254-64 pubmed publisher
	Sabri N, Roth P, Xylourgidis N, Sadeghifar F, Adler J, Samakovlis C. Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport. J Cell Biol. 2007;178:557-65 pubmed
	O Brien L, Wiese C. TPX2 is required for postmitotic nuclear assembly in cell-free Xenopus laevis egg extracts. J Cell Biol. 2006;173:685-94 pubmed
	Ulbert S, Platani M, Boue S, Mattaj I. Direct membrane protein-DNA interactions required early in nuclear envelope assembly. J Cell Biol. 2006;173:469-76 pubmed
	Wissing S, Ludovico P, Herker E, Buttner S, Engelhardt S, Decker T, et al. An AIF orthologue regulates apoptosis in yeast. J Cell Biol. 2004;166:969-74 pubmed
	Tanaka T, Serneo F, Higgins C, Gambello M, Wynshaw Boris A, Gleeson J. Lis1 and doublecortin function with dynein to mediate coupling of the nucleus to the centrosome in neuronal migration. J Cell Biol. 2004;165:709-21 pubmed
	Roth P, Xylourgidis N, Sabri N, Uv A, Fornerod M, Samakovlis C. The Drosophila nucleoporin DNup88 localizes DNup214 and CRM1 on the nuclear envelope and attenuates NES-mediated nuclear export. J Cell Biol. 2003;163:701-6 pubmed
	Kimura T, Ito C, Watanabe S, Takahashi T, Ikawa M, Yomogida K, et al. Mouse germ cell-less as an essential component for nuclear integrity. Mol Cell Biol. 2003;23:1304-15 pubmed
	Siebrasse J, Coutavas E, Peters R. Reconstitution of nuclear protein export in isolated nuclear envelopes. J Cell Biol. 2002;158:849-54 pubmed
	Drummond S, Wilson K. Interference with the cytoplasmic tail of gp210 disrupts "close apposition" of nuclear membranes and blocks nuclear pore dilation. J Cell Biol. 2002;158:53-62 pubmed
	Vasu S, Shah S, Orjalo A, Park M, Fischer W, Forbes D. Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export. J Cell Biol. 2001;155:339-54 pubmed
	Lopez Soler R, Moir R, Spann T, Stick R, Goldman R. A role for nuclear lamins in nuclear envelope assembly. J Cell Biol. 2001;154:61-70 pubmed
	Marelli M, Lusk C, Chan H, Aitchison J, Wozniak R. A link between the synthesis of nucleoporins and the biogenesis of the nuclear envelope. J Cell Biol. 2001;153:709-24 pubmed
	Heun P, Laroche T, Raghuraman M, Gasser S. The positioning and dynamics of origins of replication in the budding yeast nucleus. J Cell Biol. 2001;152:385-400 pubmed
	Faleiro L, Lazebnik Y. Caspases disrupt the nuclear-cytoplasmic barrier. J Cell Biol. 2000;151:951-9 pubmed
	Moir R, Spann T, Herrmann H, Goldman R. Disruption of nuclear lamin organization blocks the elongation phase of DNA replication. J Cell Biol. 2000;149:1179-92 pubmed
	Dreier L, Rapoport T. In vitro formation of the endoplasmic reticulum occurs independently of microtubules by a controlled fusion reaction. J Cell Biol. 2000;148:883-98 pubmed
	Davis L, Blobel G. Nuclear pore complex contains a family of glycoproteins that includes p62: glycosylation through a previously unidentified cellular pathway. Proc Natl Acad Sci U S A. 1987;84:7552-6 pubmed
	Aris J, Blobel G. Yeast nuclear envelope proteins cross react with an antibody against mammalian pore complex proteins. J Cell Biol. 1989;108:2059-67 pubmed

product information

Antigen :

Nuclear Pore Complex Proteins

Apps. Abbrev. :

Cat # :

902905

Clone :

MAb414

Item :

Direct-Blot™ HRP anti-Nuclear Pore Complex Proteins

Isotype :

Mouse IgG1

Other Names :

107 kD nucleoporin, NPC proteins, Nuclear pore complex protein Nup107, Nucleoporin 107kD, Nucleoporin Nup107, NUP 107, NUP 84, NUP107, NUP153, NUP155, NUP84, NUP98

Size :

100 μL

Price (USD) :

340 USD

Reactivity :

Human, Mouse, Rat

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

DB_HRP

Immunogen :

The antibody was raised using a nuclear pore complex mixture.

Application Notes :

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP) and immunoelectron microscopy (IEM). This antibody has been used successfully with frozen sections.*Predicted MW = 62 kDMAb414 is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis. This antibody is believed to recognize the repeated FXFG repeat sequence in nuceloporins including p62, p152 and p90 and other proteins.;