Purified anti-O-GlcNAc | BioLegend 838004 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-O-GlcNAc

catalog :

838004

quantity :

100 μg

price :

347 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CTD110.6

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

BioLegend product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1e	Guo H, Zhang B, Nairn A, Nagy T, Moremen K, Buckhaults P, et al. O-Linked N-Acetylglucosamine (O-GlcNAc) Expression Levels Epigenetically Regulate Colon Cancer Tumorigenesis by Affecting the Cancer Stem Cell Compartment via Modulating Expression of Transcriptional Factor MYBL1. J Biol Chem. 2017;292:4123-4137 pubmed publisher
western blot; human; loading ...; fig 1b	Someya A, Ikegami T, Sakamoto K, Nagaoka I. Glucosamine Downregulates the IL-1?-Induced Expression of Proinflammatory Cytokine Genes in Human Synovial MH7A Cells by O-GlcNAc Modification-Dependent and -Independent Mechanisms. PLoS ONE. 2016;11:e0165158 pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 4a western blot; mouse; fig 1b	Borghgraef P, Menuet C, Theunis C, Louis J, Devijver H, Maurin H, et al. Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and mortality of Tau.P301L mice. PLoS ONE. 2013;8:e84442 pubmed publisher
	Basehore S, Bohlman S, Weber C, Swaminathan S, Zhang Y, Jang C, et al. Laminar Flow on Endothelial Cells Suppresses eNOS O-GlcNAcylation to Promote eNOS Activity. Circ Res. 2021;129:1054-1066 pubmed publisher
	Kuo W, Tseng L, Chang C, Chen C, Cheng M, Cheng H, et al. Prognostic Significance of O-GlcNAc and PKM2 in Hormone Receptor-Positive and HER2-Nonenriched Breast Cancer. Diagnostics (Basel). 2021;11: pubmed publisher
	Liu Y, Chen Q, Zhang N, Zhang K, Dou T, Cao Y, et al. Proteomic profiling and genome-wide mapping of O-GlcNAc chromatin-associated proteins reveal an O-GlcNAc-regulated genotoxic stress response. Nat Commun. 2020;11:5898 pubmed publisher
	Seo H, Kim H, Yoon J, Kweon T, Park Y, Kang J, et al. Mutual regulation between OGT and XIAP to control colon cancer cell growth and invasion. Cell Death Dis. 2020;11:815 pubmed publisher
	Cai B, Dongiovanni P, Corey K, Wang X, Shmarakov I, Zheng Z, et al. Macrophage MerTK Promotes Liver Fibrosis in Nonalcoholic Steatohepatitis. Cell Metab. 2020;31:406-421.e7 pubmed publisher
	Durst M, Ratia K, Lavie A. Identifying small molecule probes of ENTPD5 through high throughput screening. PLoS ONE. 2019;14:e0210305 pubmed publisher
	Ligorio M, Sil S, Malagon Lopez J, Nieman L, Misale S, Di Pilato M, et al. Stromal Microenvironment Shapes the Intratumoral Architecture of Pancreatic Cancer. Cell. 2019;: pubmed publisher
	Choi H, Kim C, Song H, Cha M, Cho H, Son S, et al. Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death. Aging Cell. 2019;18:e12872 pubmed publisher
	Hao X, Li Y, Wang J, Ma J, Zhao S, Ye X, et al. Deficient O-GlcNAc Glycosylation Impairs Regulatory T Cell Differentiation and Notch Signaling in Autoimmune Hepatitis. Front Immunol. 2018;9:2089 pubmed publisher
	Nugent B, O Donnell C, Epperson C, Bale T. Placental H3K27me3 establishes female resilience to prenatal insults. Nat Commun. 2018;9:2555 pubmed publisher
	Jean Louis T, Rockwell P, Figueiredo Pereira M. Prostaglandin J2 promotes O-GlcNAcylation raising APP processing by α- and β-secretases: relevance to Alzheimer's disease. Neurobiol Aging. 2018;62:130-145 pubmed publisher
	Kim N, Cha Y, Lee J, Lee S, Yang J, Yun J, et al. Snail reprograms glucose metabolism by repressing phosphofructokinase PFKP allowing cancer cell survival under metabolic stress. Nat Commun. 2017;8:14374 pubmed publisher
	Chen Y, Chen C, Liang C, Liang J, Tai M, Chang Y, et al. Silibinin inhibits ICAM-1 expression via regulation of N-linked and O-linked glycosylation in ARPE-19 cells. Biomed Res Int. 2014;2014:701395 pubmed publisher

product information

Antigen :

O-GlcNAc

Apps. Abbrev. :

WB, ICC, IHC-P, IP

Cat # :

838004

Clone :

CTD110.6

Item :

Purified anti-O-GlcNAc

Isotype :

Mouse IgM, κ

Other Names :

O-Linked N-Acetylglucosamine

Size :

100 μg

Price (USD) :

347 USD

Reactivity :

All Species

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PURE

Immunogen :

The antibody was raised against a synthetic peptide containing Serine-O-GlcNAc.

Application Notes :

This antibody detects Ser-O-GlcNAc and Thr-O-GlcNAc but shows no cross-reactivity with peptide determinants or other closely-related carbohydrate antigens. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc. Milk proteins may cross-react with the antibody therefore we recommend using BSA or goat serum as a blocker.Additional reported applications (for the relevant formats) include: ELISA^2, immunocytochemistry^7, immunoprecipitation^2,5,6, and Western blotting^3,4,7.;

more info or order :

BioLegend product webpage