Anti-O-GlcNAc | BioLegend 838002 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Anti-O-GlcNAc

catalog :

838002

quantity :

500 μl

price :

381 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CTD110.6

reactivity :

, mouse, rat, fruit fly

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 7

Published Application/Species/Dilution	Reference
western blot; fruit fly	Park S, Lee Y, Pak J, Kim H, Choi H, Kim J, et al. O-GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster. Cell Mol Life Sci. 2015;72:3173-83 pubmed publisher
western blot; rat	Hortemo K, Aronsen J, Lunde I, Sjaastad I, Lunde P, Sejersted O. Exhausting treadmill running causes dephosphorylation of sMLC2 and reduced level of myofilament MLCK2 in slow twitch rat soleus muscle. Physiol Rep. 2015;3: pubmed publisher
western blot; mouse immunohistochemistry; mouse	Borghgraef P, Menuet C, Theunis C, Louis J, Devijver H, Maurin H, et al. Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and mortality of Tau.P301L mice. PLoS ONE. 2013;8:e84442 pubmed publisher
western blot; mouse immunoprecipitation; mouse	Shi F, Kim H, Lu W, He Q, Liu D, Goodell M, et al. Ten-eleven translocation 1 (Tet1) is regulated by O-linked N-acetylglucosamine transferase (Ogt) for target gene repression in mouse embryonic stem cells. J Biol Chem. 2013;288:20776-84 pubmed publisher
	Hortemo K, Lunde P, Anonsen J, Kvaløy H, Munkvik M, Rehn T, et al. Exercise training increases protein O-GlcNAcylation in rat skeletal muscle. Physiol Rep. 2016;4: pubmed
	Anthonisen E, Berven L, Holm S, Nygard M, Nebb H, Grønning-Wang L. Nuclear receptor liver X receptor is O-GlcNAc-modified in response to glucose. J Biol Chem. 2010;285:1607-15 pubmed publisher
	Dias W, Cheung W, Wang Z, Hart G. Regulation of calcium/calmodulin-dependent kinase IV by O-GlcNAc modification. J Biol Chem. 2009;284:21327-37 pubmed publisher

product information

Antigen :

O-GlcNAc

Apps. Abbrev. :

Western Blotting, ELISA, IP, ICC, Immunofluorescence Microscopy

Cat # :

838002

Clone :

CTD110.6

Item :

Anti-O-GlcNAc

Isotype :

Mouse IgM

Other Names :

β-O-linked N-acetylglucosamine, MMS-248R-500

Size :

500 μl

Price (USD) :

381 USD

Reactivity :

All Species

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

Ascites

Immunogen :

The CTD110.6 antibody was raised against a synthetic peptide containing Serine-O-GlcNAc.

Application Notes :

This antibody is effective in ELISA, immunoblotting (WB) and immunoprecipitation (IP). *Milk proteins may cross-react with the antibody therefore we recommend using BSA or goat serum as a blocker. This monoclonal antibody represents a powerful new tool for the study of O-GlcNAc's role in cell physiology. This antibody detects Ser-O-GlcNAc and Thr-O-GlcNAc but shows no cross-reactivity with peptide determinants or other closely-related carbohydrate antigens. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc.