Anti-Neurofilament Marker (pan axonal, cocktail) | BioLegend 837901 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Anti-Neurofilament Marker (pan axonal, cocktail)

catalog :

837901

quantity :

100 μl

price :

320 USD

clonality :

monoclonal

host :

mouse

reactivity :

human, mouse, rat, dog

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 7

immunocytochemistry; mouse	Hsu W, Chung H, Wu C, Wu H, Lee Y, Chen E, et al. Glutamate Stimulates Local Protein Synthesis in the Axons of Rat Cortical Neurons by Activating α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors and Metabotropic Glutamate Receptors. J Biol Chem. 2015;290:20748-60 pubmed publisher
immunohistochemistry; dog; 1:1000; fig 3	Kegler K, Spitzbarth I, Imbschweiler I, Wewetzer K, Baumgartner W, Seehusen F. Contribution of Schwann Cells to Remyelination in a Naturally Occurring Canine Model of CNS Neuroinflammation. PLoS ONE. 2015;10:e0133916 pubmed publisher
immunocytochemistry; rat; 1:5000	Heinen A, Beyer F, Tzekova N, Hartung H, Küry P. Fingolimod induces the transition to a nerve regeneration promoting Schwann cell phenotype. Exp Neurol. 2015;271:25-35 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Peters O, Shelkovnikova T, Highley J, Cooper-Knock J, Hortobagyi T, Troakes C, et al. Gamma-synuclein pathology in amyotrophic lateral sclerosis. Ann Clin Transl Neurol. 2015;2:29-37 pubmed publisher
immunohistochemistry; mouse; 1:500	Moreno M, Bannerman P, Ma J, Guo F, Miers L, Soulika A, et al. Conditional ablation of astroglial CCL2 suppresses CNS accumulation of M1 macrophages and preserves axons in mice with MOG peptide EAE. J Neurosci. 2014;34:8175-85 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000	Chen J, Mizushige T, Nishimune H. Active zone density is conserved during synaptic growth but impaired in aged mice. J Comp Neurol. 2012;520:434-52 pubmed publisher
immunohistochemistry; rat; 1:1,000	Petralia R, Schwartz C, Wang Y, Mattson M, Yao P. Subcellular localization of Patched and Smoothened, the receptors for Sonic hedgehog signaling, in the hippocampal neuron. J Comp Neurol. 2011;519:3684-99 pubmed publisher

product information

Antigen :

Neurofilament Marker

Apps. Abbrev. :

IHC, Western Blotting, ELISA, ICC, Immunofluorescence Microscopy

Cat # :

837901

Clone :

SMI 312

Item :

Anti-Neurofilament Marker (pan axonal, cocktail)

Isotype :

Mouse IgG1/Mouse IgM

Other Names :

SMI-312R-100

Size :

100 μl

Price (USD) :

320 USD

Reactivity :

Mammalian

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

Ascites

Application Notes :

SMI 312 is a mixture of monoclonals that immunolabel complex networks of axons. It is directed against extensively phosphorylated axonal epitopes on NF-M and NF-H. This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC) and ELISA. Positive Control: human cerebellum tissue SMI 312 has been selected to provide a specific marker for axons in tissue sections and cultures. In contrast to individual antiphosphoneurofilaments that identify different subsets of neurofilament phosphoepitopes, and are, therefore, especially suitable for defining functional and regional differences in normal and pathologic axons, SMI 312 is a convenient marker for axons in general. SMI 312 visualizes axons in an area-specific maturation pattern in human fetal brain. The antibody cocktail defines nuclear borderlines and is, thereby, useful in establishing early connectivities with SMI 311, antinonphosphoneurofilament-identified dendrites. SMI 312 visualizes aberrantly sprouting axons in neuritic plaques derived from cortico-cortical fibers in Alzheimer's disease and identifies loss of synaptic circuitry proposed to be the basis of memory