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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Anti-GFAP
catalog :
837202
quantity :
500 μL
price :
483 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SMI 21
reactivity :
human, mouse, dogs
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 19
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; human; 1:500; loading ...; fig 2e
Li J, Pan L, Pembroke W, Rexach J, Godoy M, Condro M, et al. Conservation and divergence of vulnerability and responses to stressors between human and mouse astrocytes. Nat Commun. 2021;12:3958 pubmed publisher
  • immunocytochemistry; human; 1:400; loading ...; tbl 1
  • western blot; human; 1:5000; loading ...; tbl 1
  • immunocytochemistry; mouse; 1:400; loading ...; tbl 1
  • western blot; mouse; 1:5000; loading ...; tbl 1
Lin N, Messing A, Perng M. Characterization of a panel of monoclonal antibodies recognizing specific epitopes on GFAP. PLoS ONE. 2017;12:e0180694 pubmed publisher
  • western blot; human; fig 3a
Jung J, Kim L, Wang X, Wu Q, Sanvoranart T, Hubert C, et al. Nicotinamide metabolism regulates glioblastoma stem cell maintenance. JCI Insight. 2017;2: pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...; fig s4c
Ehrlich M, Mozafari S, Glatza M, Starost L, Velychko S, Hallmann A, et al. Rapid and efficient generation of oligodendrocytes from human induced pluripotent stem cells using transcription factors. Proc Natl Acad Sci U S A. 2017;114:E2243-E2252 pubmed publisher
  • immunocytochemistry; human; 1:000; fig 4
McClain C, Sim F, Goldman S. Pleiotrophin suppression of receptor protein tyrosine phosphatase-?/? maintains the self-renewal competence of fetal human oligodendrocyte progenitor cells. J Neurosci. 2012;32:15066-75 pubmed publisher
Xu A, Liu M, Huang M, Zhang Y, Hu R, Gingold J, et al. Rewired m6A epitranscriptomic networks link mutant p53 to neoplastic transformation. Nat Commun. 2023;14:1694 pubmed publisher
Wiley C, Steinman R, Wang Q. Innate immune activation without immune cell infiltration in brains of murine models of Aicardi-Goutières Syndrome. Brain Pathol. 2023;33:e13118 pubmed publisher
Guo X, Steinman R, Sheng Y, Cao G, Wiley C, Wang Q. An AGS-associated mutation in ADAR1 catalytic domain results in early-onset and MDA5-dependent encephalopathy with IFN pathway activation in the brain. J Neuroinflammation. 2022;19:285 pubmed publisher
Shah D, Gsell W, Wahis J, Luckett E, Jamoulle T, Vermaercke B, et al. Astrocyte calcium dysfunction causes early network hyperactivity in Alzheimer's disease. Cell Rep. 2022;40:111280 pubmed publisher
Padmashri R, Ren B, Oldham B, Jung Y, Gough R, Dunaevsky A. Modeling human-specific interlaminar astrocytes in the mouse cerebral cortex. J Comp Neurol. 2021;529:802-810 pubmed publisher
Lutgen V, Narasipura S, Barbian H, Richards M, Wallace J, Razmpour R, et al. HIV infects astrocytes in vivo and egresses from the brain to the periphery. PLoS Pathog. 2020;16:e1008381 pubmed publisher
Wang J, Saraswat D, Sinha A, Polanco J, Dietz K, O Bara M, et al. Paired Related Homeobox Protein 1 Regulates Quiescence in Human Oligodendrocyte Progenitors. Cell Rep. 2018;25:3435-3450.e6 pubmed publisher
Koeppen A, Becker A, Qian J, Gelman B, Mazurkiewicz J. Friedreich Ataxia: Developmental Failure of the Dorsal Root Entry Zone. J Neuropathol Exp Neurol. 2017;76:969-977 pubmed publisher
Windrem M, Osipovitch M, Liu Z, Bates J, Chandler Militello D, Zou L, et al. Human iPSC Glial Mouse Chimeras Reveal Glial Contributions to Schizophrenia. Cell Stem Cell. 2017;21:195-208.e6 pubmed publisher
Park N, Guilhamon P, Desai K, McAdam R, Langille E, O Connor M, et al. ASCL1 Reorganizes Chromatin to Direct Neuronal Fate and Suppress Tumorigenicity of Glioblastoma Stem Cells. Cell Stem Cell. 2017;21:209-224.e7 pubmed publisher
Benraiss A, Wang S, Herrlinger S, Li X, Chandler Militello D, Mauceri J, et al. Human glia can both induce and rescue aspects of disease phenotype in Huntington disease. Nat Commun. 2016;7:11758 pubmed publisher
Choi V, Bigelow C, McGee T, Gujar A, Li H, Hanks S, et al. AAV-mediated RLBP1 gene therapy improves the rate of dark adaptation in Rlbp1 knockout mice. Mol Ther Methods Clin Dev. 2015;2:15022 pubmed publisher
Chen M, Hagemann T, Quinlan R, Messing A, Perng M. Caspase cleavage of GFAP produces an assembly-compromised proteolytic fragment that promotes filament aggregation. ASN Neuro. 2013;5:e00125 pubmed publisher
Chort A, Alves S, Marinello M, Dufresnois B, Dornbierer J, Tesson C, et al. Interferon ? induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice. Brain. 2013;136:1732-45 pubmed publisher
product information
Antigen :
GFAP
Apps. Abbrev. :
IHC-P, WB, ICC
Cat # :
837202
Clone :
SMI 21
Item :
Anti-GFAP
Isotype :
Mouse IgG1, κ
Other Names :
Glial fibrillary acidic protein
Size :
500 μL
Price (USD) :
483 USD
Reactivity :
Human, Non-Human Primate, Dog
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
ASCITES
Application Notes :
SMI 21 has been tested for immunocytochemical localization of GFAP in astrocytes and Bergmann glia in human, monkey and dog paraffin sections. Astrocytomas from human and dog show a positive reaction with SMI 21. Rat, rabbit and mouse brain sections do not react with SMI 21. Other species have not been examined. No reactivity was observed on liver and kidney paraffin sections. SMI 21 reacts with GFAP and shows no cross reactivity with other intermediate filaments on tissue sections or Western blots of human brain proteins. Rat cytoskeletal proteins do not react with SMI 21.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA