Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

Purified anti-Neurofilament H (NF-H), Phosphorylated

835504

25 μg

112 USD

monoclonal

mouse

nonconjugated

SMI 34

human, mouse, rat

western blot, immunohistochemistry - paraffin section

IHC-P, WB

835504

SMI 34

Purified anti-Neurofilament H (NF-H), Phosphorylated

Mouse IgG1, κ

Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein

25 μg

112 USD

Human, Mouse, Rat

Monoclonal

Mouse

PURE

SMI 34 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, as well as in chicken. Immunohistochemically, SMI 34 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 34. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 34 may be found also in neuronal cell bodies. In Alzheimer's disease, SMI 34 reacts with intraneuronal tangles but does not react with extraneuronal (ghost) tangles. It is the only anti-phosphoneurofilament that reacts regularly with Alzheimer tangles. It does not cross-react at concentrations of 1:20,000 with Alzheimer MAP-Tau in immunoblots, demonstrating, thereby, the presence of neurofilaments in Alzheimer tangles.;