product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified (azide-free) anti-β-Amyloid, 1-42
catalog :
805502
quantity :
500 μL
price :
1035 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
12F4
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
more info or order :
citations: 45
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - free floating section; mouse; loading ...; fig 1e
Ma C, Hunt J, Selenica M, Sanneh A, Sandusky Beltran L, Watler M, et al. Arginase 1 Insufficiency Precipitates Amyloid-β Deposition and Hastens Behavioral Impairment in a Mouse Model of Amyloidosis. Front Immunol. 2020;11:582998 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:1000; loading ...; fig 5a
  • western blot; mouse; 1:1000
Klementieva O, Willén K, Martinsson I, Israelsson B, Engdahl A, Cladera J, et al. Pre-plaque conformational changes in Alzheimer's disease-linked Aβ and APP. Nat Commun. 2017;8:14726 pubmed publisher
  • ELISA; human; 1 ug/ml; fig 6
Miners J, Palmer J, Love S. Pathophysiology of Hypoperfusion of the Precuneus in Early Alzheimer's Disease. Brain Pathol. 2016;26:533-41 pubmed publisher
  • western blot; mouse; 1:500; fig 4
Owais K, Huang T, Mahmood F, Hubbard J, Saraf R, Bardia A, et al. Cardiopulmonary Bypass Decreases Activation of the Signal Transducer and Activator of Transcription 3 (STAT3) Pathway in Diabetic Human Myocardium. Ann Thorac Surg. 2015;100:1636-45; discussion 1645 pubmed publisher
  • western blot; mouse; 1:500; fig 4
Tousseyn T, Bajsarowicz K, Sánchez H, Gheyara A, Oehler A, Geschwind M, et al. Prion Disease Induces Alzheimer Disease-Like Neuropathologic Changes. J Neuropathol Exp Neurol. 2015;74:873-88 pubmed publisher
  • ELISA; human; 1:500
Ashby E, Miners J, Kumar S, Walter J, Love S, Kehoe P. Investigation of Aβ phosphorylated at serine 8 (pAβ) in Alzheimer's disease, dementia with Lewy bodies and vascular dementia. Neuropathol Appl Neurobiol. 2015;41:428-44 pubmed publisher
Willumsen N, Arber C, Lovejoy C, Toombs J, Alatza A, Weston P, et al. The PSEN1 E280G mutation leads to increased amyloid-β43 production in induced pluripotent stem cell neurons and deposition in brain tissue. Brain Commun. 2023;5:fcac321 pubmed publisher
Jin J, Xu Z, Zhang L, Zhang C, Zhao X, Mao Y, et al. Gut-derived β-amyloid: Likely a centerpiece of the gut-brain axis contributing to Alzheimer's pathogenesis. Gut Microbes. 2023;15:2167172 pubmed publisher
Nick H, Fenik P, Zhu Y, Veasey S. Hypocretin/orexin influences chronic sleep disruption injury in the hippocampus. Front Aging Neurosci. 2022;14:1025402 pubmed publisher
Piccirillo S, Preziuso A, Amoroso S, Serfilippi T, Miceli F, Magi S, et al. A new K+channel-independent mechanism is involved in the antioxidant effect of XE-991 in an in vitro model of glucose metabolism impairment: implications for Alzheimer's disease. Cell Death Discov. 2022;8:391 pubmed publisher
Bishay J, Beckett T, Lai A, Hill M, McMahon D, McLaurin J. Venular amyloid accumulation in transgenic Fischer 344 Alzheimer's disease rats. Sci Rep. 2022;12:15287 pubmed publisher
Gutknecht M, Kaku H, Rothstein T. Microparticle immunocapture assay for quantitation of protein multimer amount and size. Cell Rep Methods. 2022;2:100214 pubmed publisher
Plowey E, Bussiere T, Rajagovindan R, Sebalusky J, Hamann S, von Hehn C, et al. Alzheimer disease neuropathology in a patient previously treated with aducanumab. Acta Neuropathol. 2022;144:143-153 pubmed publisher
Jord xe0 Siquier T, Petrel M, Kouskoff V, Smailovic U, Cordeli xe8 res F, Frykman S, et al. APP accumulates with presynaptic proteins around amyloid plaques: A role for presynaptic mechanisms in Alzheimer's disease?. Alzheimers Dement. 2022;18:2099-2116 pubmed publisher
Maxwell A, Yuan P, Rivera B, Schaaf W, Mladinov M, Prasher V, et al. Emergence of distinct and heterogeneous strains of amyloid beta with advanced Alzheimer's disease pathology in Down syndrome. Acta Neuropathol Commun. 2021;9:201 pubmed publisher
Koh H, Lee S, Lee H, Min J, Iwatsubo T, Teunissen C, et al. Targeting MicroRNA-485-3p Blocks Alzheimer's Disease Progression. Int J Mol Sci. 2021;22: pubmed publisher
Kim H, Kim S, Park S, Park G, Shin H, Park M, et al. Administration of Bifidobacterium bifidum BGN4 and Bifidobacterium longum BORI Improves Cognitive and Memory Function in the Mouse Model of Alzheimer's Disease. Front Aging Neurosci. 2021;13:709091 pubmed publisher
Ruiz Riquelme A, Mao A, Barghash M, Lau H, Stuart E, Kovacs G, et al. Aβ43 aggregates exhibit enhanced prion-like seeding activity in mice. Acta Neuropathol Commun. 2021;9:83 pubmed publisher
Owen J, Zhu Y, Fenik P, Zhan G, Bell P, Liu C, et al. Late-in-life neurodegeneration after chronic sleep loss in young adult mice. Sleep. 2021;44: pubmed publisher
Olah M, Menon V, Habib N, Taga M, Ma Y, Yung C, et al. Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer's disease. Nat Commun. 2020;11:6129 pubmed publisher
Li K, Jiang Y, Li G, Liu T, Yang Z. Novel Multitarget Directed Tacrine Hybrids as Anti-Alzheimer's Compounds Improved Synaptic Plasticity and Cognitive Impairment in APP/PS1 Transgenic Mice. ACS Chem Neurosci. 2020;11:4316-4328 pubmed publisher
Barbato C, Giacovazzo G, Albiero F, Scardigli R, Scopa C, Ciotti M, et al. Cognitive Decline and Modulation of Alzheimer's Disease-Related Genes After Inhibition of MicroRNA-101 in Mouse Hippocampal Neurons. Mol Neurobiol. 2020;57:3183-3194 pubmed publisher
Parker H, Ellison S, Holley R, O Leary C, Liao A, Asadi J, et al. Haematopoietic stem cell gene therapy with IL-1Ra rescues cognitive loss in mucopolysaccharidosis IIIA. EMBO Mol Med. 2020;12:e11185 pubmed publisher
Shi H, Koronyo Y, Rentsendorj A, Regis G, Sheyn J, Fuchs D, et al. Identification of early pericyte loss and vascular amyloidosis in Alzheimer's disease retina. Acta Neuropathol. 2020;139:813-836 pubmed publisher
Kim K, Kim M, Kim D, Kim S, Park S, Park C. Clinically accurate diagnosis of Alzheimer's disease via multiplexed sensing of core biomarkers in human plasma. Nat Commun. 2020;11:119 pubmed publisher
Chan H, Xu D, Ho S, He D, Wong M, Li H. Highly sensitive quantification of Alzheimer's disease biomarkers by aptamer-assisted amplification. Theranostics. 2019;9:2939-2949 pubmed publisher
Ruiz Riquelme A, Lau H, Stuart E, Goczi A, Wang Z, Schmitt Ulms G, et al. Prion-like propagation of β-amyloid aggregates in the absence of APP overexpression. Acta Neuropathol Commun. 2018;6:26 pubmed publisher
Lee C, Daggett A, Gu X, Jiang L, Langfelder P, Li X, et al. Elevated TREM2 Gene Dosage Reprograms Microglia Responsivity and Ameliorates Pathological Phenotypes in Alzheimer's Disease Models. Neuron. 2018;97:1032-1048.e5 pubmed publisher
Kommaddi R, Das D, Karunakaran S, Nanguneri S, Bapat D, Ray A, et al. Aβ mediates F-actin disassembly in dendritic spines leading to cognitive deficits in Alzheimer's disease. J Neurosci. 2018;38:1085-1099 pubmed publisher
Kim C, Park J, Kong T, Lee S, Seo K, Choi Y, et al. Double-Injected Human Stem Cells Enhance Rehabilitation in TBI Mice Via Modulation of Survival and Inflammation. Mol Neurobiol. 2018;55:4870-4884 pubmed publisher
Moreno F, Indakoetxea B, Barandiaran M, Caballero M, Gorostidi A, Calafell F, et al. The unexpected co-occurrence of GRN and MAPT p.A152T in Basque families: Clinical and pathological characteristics. PLoS ONE. 2017;12:e0178093 pubmed publisher
Ahmad F, Singh K, Das D, Gowaikar R, Shaw E, Ramachandran A, et al. Reactive Oxygen Species-Mediated Loss of Synaptic Akt1 Signaling Leads to Deficient Activity-Dependent Protein Translation Early in Alzheimer's Disease. Antioxid Redox Signal. 2017;27:1269-1280 pubmed publisher
Augé E, Cabezón I, Pelegri C, Vilaplana J. New perspectives on corpora amylacea in the human brain. Sci Rep. 2017;7:41807 pubmed publisher
Savage M, Kalinina J, Wolfe A, Tugusheva K, Korn R, Cash Mason T, et al. A sensitive a? oligomer assay discriminates Alzheimer's and aged control cerebrospinal fluid. J Neurosci. 2014;34:2884-97 pubmed publisher
Eimer W, Vassar R. Neuron loss in the 5XFAD mouse model of Alzheimer's disease correlates with intraneuronal A?42 accumulation and Caspase-3 activation. Mol Neurodegener. 2013;8:2 pubmed publisher
Helwig M, Hoshino A, Berridge C, Lee S, Lorenzen N, Otzen D, et al. The neuroendocrine protein 7B2 suppresses the aggregation of neurodegenerative disease-related proteins. J Biol Chem. 2013;288:1114-24 pubmed publisher
Sundaram R, Kasinathan C, Stein S, SUNDARAM P. Novel Detox Gel Depot sequesters ?-Amyloid Peptides in a mouse model of Alzheimer's Disease. Int J Pept Res Ther. 2012;18:99-106 pubmed
Ohmi K, Zhao H, Neufeld E. Defects in the medial entorhinal cortex and dentate gyrus in the mouse model of Sanfilippo syndrome type B. PLoS ONE. 2011;6:e27461 pubmed publisher
Cenini G, Dowling A, Beckett T, Barone E, Mancuso C, Murphy M, et al. Association between frontal cortex oxidative damage and beta-amyloid as a function of age in Down syndrome. Biochim Biophys Acta. 2012;1822:130-8 pubmed publisher
Maheshwari M, Roberts J, Desutter B, Duong K, Tingling J, Fawver J, et al. Hydralazine modifies A? fibril formation and prevents modification by lipids in vitro. Biochemistry. 2010;49:10371-80 pubmed publisher
Vingtdeux V, Chandakkar P, Zhao H, d Abramo C, Davies P, Marambaud P. Novel synthetic small-molecule activators of AMPK as enhancers of autophagy and amyloid-? peptide degradation. FASEB J. 2011;25:219-31 pubmed publisher
Townsend M, Qu Y, Gray A, Wu Z, Seto T, Hutton M, et al. Oral treatment with a gamma-secretase inhibitor improves long-term potentiation in a mouse model of Alzheimer's disease. J Pharmacol Exp Ther. 2010;333:110-9 pubmed publisher
Takami M, Nagashima Y, Sano Y, Ishihara S, Morishima Kawashima M, Funamoto S, et al. gamma-Secretase: successive tripeptide and tetrapeptide release from the transmembrane domain of beta-carboxyl terminal fragment. J Neurosci. 2009;29:13042-52 pubmed publisher
Sudol K, Mastrangelo M, Narrow W, Frazer M, Levites Y, Golde T, et al. Generating differentially targeted amyloid-beta specific intrabodies as a passive vaccination strategy for Alzheimer's disease. Mol Ther. 2009;17:2031-40 pubmed publisher
Mastrangelo M, Bowers W. Detailed immunohistochemical characterization of temporal and spatial progression of Alzheimer's disease-related pathologies in male triple-transgenic mice. BMC Neurosci. 2008;9:81 pubmed publisher
product information
Antigen :
beta-Amyloid 1-42
Apps. Abbrev. :
IHC-P, Direct ELISA, WB, IHC-F
Cat # :
805502
Clone :
12F4
Item :
Purified (azide-free) anti-β-Amyloid, 1-42
Isotype :
Mouse IgG1, κ
Other Names :
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Size :
500 μL
Price (USD) :
1035 USD
Reactivity :
Human, Mouse, Rat
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PUREAF
Application Notes :
This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), and ELISA.This antibody is reactive to the C-terminus of beta amyloid and is specific for the isoform ending at the 42nd amino acid.Additional reported applications (for the relevant formats) includes: immunohistochemical staining of paraformaldehyde-fixed frozen sections^6 and ELISA^3,4,5.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA