Anti-Alpha-II Spectrin | BioLegend 803201 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Anti-Alpha-II Spectrin

catalog :

803201

quantity :

200 μL

price :

581 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

D8B7

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, western blot knockout validation, immunocytochemistry knockout validation

more info or order :

BioLegend product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry knockout validation; mouse; loading ...; fig 2h, 2j western blot knockout validation; mouse; loading ...; fig 2b western blot; human; loading ...; fig 1a	Lubbers E, Murphy N, Musa H, Huang C, Gupta R, Price M, et al. Defining new mechanistic roles for αII spectrin in cardiac function. J Biol Chem. 2019;294:9576-9591 pubmed publisher
western blot; mouse; 1:250; loading ...; fig 2f	Wang G, Simon D, Wu Z, Belsky D, Heller E, O Rourke M, et al. Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration. elife. 2019;8: pubmed publisher
	Cousin M, Creighton B, Breau K, Spillmann R, Torti E, Dontu S, et al. Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome. Nat Genet. 2021;53:1006-1021 pubmed publisher
	Miazek A, Zalas M, Skrzymowska J, Bogin B, Grzymajło K, Goszczyński T, et al. Age-dependent ataxia and neurodegeneration caused by an αII spectrin mutation with impaired regulation of its calpain sensitivity. Sci Rep. 2021;11:7312 pubmed publisher
	Hamdan H, Lim B, Torii T, Joshi A, Konning M, Smith C, et al. Mapping axon initial segment structure and function by multiplexed proximity biotinylation. Nat Commun. 2020;11:100 pubmed publisher
	Torii T, Ogawa Y, Liu C, Ho T, Hamdan H, Wang C, et al. NuMA1 promotes axon initial segment assembly through inhibition of endocytosis. J Cell Biol. 2020;219: pubmed publisher
	Liu Y, Qi J, Chen X, Tang M, Chu C, Zhu W, et al. Critical role of spectrin in hearing development and deafness. Sci Adv. 2019;5:eaav7803 pubmed publisher
	Huang C, Zhang C, Zollinger D, Leterrier C, Rasband M. An αII Spectrin-Based Cytoskeleton Protects Large-Diameter Myelinated Axons from Degeneration. J Neurosci. 2017;37:11323-11334 pubmed publisher
	Huang C, Zhang C, Ho T, Oses Prieto J, Burlingame A, Lalonde J, et al. αII Spectrin Forms a Periodic Cytoskeleton at the Axon Initial Segment and Is Required for Nervous System Function. J Neurosci. 2017;37:11311-11322 pubmed publisher

product information

Antigen :

alpha-II Spectrin

Apps. Abbrev. :

WB, IHC-P, ICC

Cat # :

803201

Clone :

D8B7

Item :

Anti-Alpha-II Spectrin

Isotype :

Mouse IgG2b, κ

Other Names :

Spectrin alpha chain, brain, alpha-II spectrin, fodrin alpha chain, spectrin, non-erythroid alpha chain, EL2, SPTA1

Size :

200 μL

Price (USD) :

581 USD

Reactivity :

Human, Mouse, Rat, Drosophila

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

ASCITES

Application Notes :

Clone D8B7 is a specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000)^4, 6, 8. It does not cross-react with alpha I (erythroid) spectrin SH3 domain.This antibody was developed against a recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain. It was subsequently purified via ammonium sulfate precipitation.Reactivity to Drosophila was only verified with the purified format.;

more info or order :

BioLegend product webpage