Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

Alexa Fluor® 594 anti-Neurofilament H (NF-H), Nonphosphorylated

801710

100 μg

323 USD

monoclonal

mouse

nonconjugated

SMI 32

human, mouse, rat

immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

IHC-P, ICC, IHC-F

801710

SMI 32

Alexa Fluor® 594 anti-Neurofilament H (NF-H), Nonphosphorylated

Mouse IgG1

Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein

100 μg

323 USD

Human, Mouse, Rat

Monoclonal

Mouse

A594

Additional reported applications (for the relevant formats) include Western blotting^6, immunohistochemistry^4,5, immunocytochemistry^1,2,3, 7, array tomography^8.Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house^4.This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca^2+ uptake.;