product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified (azide-free) anti-Neurofilament H (NF-H), Nonphosphorylated
catalog :
801701
quantity :
100 μL
price :
259 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
SMI 32
reactivity :
African green monkey, grey mouse lemur, human, mouse, rat, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
more info or order :
citations: 161
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 3a
Groh J, Berve K, Martini R. Immune modulation attenuates infantile neuronal ceroid lipofuscinosis in mice before and after disease onset. Brain Commun. 2021;3:fcab047 pubmed publisher
  • immunohistochemistry; mouse; 1:3000; loading ...; fig 5d
Sherafat A, Pfeiffer F, Reiss A, Wood W, Nishiyama A. Microglial neuropilin-1 promotes oligodendrocyte expansion during development and remyelination by trans-activating platelet-derived growth factor receptor. Nat Commun. 2021;12:2265 pubmed publisher
  • immunohistochemistry; human; loading ...; fig 3d
Miguel J, Perez S, Malek Ahmadi M, Mufson E. Cerebellar Calcium-Binding Protein and Neurotrophin Receptor Defects in Down Syndrome and Alzheimer's Disease. Front Aging Neurosci. 2021;13:645334 pubmed publisher
  • immunohistochemistry - free floating section; rat; 1:100; loading ...; fig 7e
  • immunohistochemistry - free floating section; mouse; 1:100; loading ...; fig 5c
Zhang L, Pan J, Mamtilahun M, Zhu Y, Wang L, Venkatesh A, et al. Microglia exacerbate white matter injury via complement C3/C3aR pathway after hypoperfusion. Theranostics. 2020;10:74-90 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2c
Yang C, Wang X, Wang J, Wang X, Chen W, Lu N, et al. Rewiring Neuronal Glycerolipid Metabolism Determines the Extent of Axon Regeneration. Neuron. 2020;105:276-292.e5 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 3a3
Reinhard K, Li C, Do Q, Burke E, Heynderickx S, Farrow K. A projection specific logic to sampling visual inputs in mouse superior colliculus. elife. 2019;8: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 8i
Jiang D, Burger C, Casasent A, Albrecht N, Li F, Samuel M. Spatiotemporal gene expression patterns reveal molecular relatedness between retinal laminae. J Comp Neurol. 2019;: pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:500; loading ...; fig 2a
Zhang Q, Zhu W, Xu F, Dai X, Shi L, Cai W, et al. The interleukin-4/PPARγ signaling axis promotes oligodendrocyte differentiation and remyelination after brain injury. PLoS Biol. 2019;17:e3000330 pubmed publisher
  • immunohistochemistry - frozen section; grey mouse lemur; 1:2000
Saraf M, Balaram P, Pifferi F, Kennedy H, Kaas J. The sensory thalamus and visual midbrain in mouse lemurs. J Comp Neurol. 2019;527:2599-2611 pubmed publisher
  • immunohistochemistry; grey mouse lemur; 1:2000; loading ...; fig 3c
Saraf M, Balaram P, Pifferi F, Gămănuţ R, Kennedy H, Kaas J. Architectonic features and relative locations of primary sensory and related areas of neocortex in mouse lemurs. J Comp Neurol. 2019;527:625-639 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig st1
Ku T, Swaney J, Park J, Albanese A, Murray E, Cho J, et al. Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues. Nat Biotechnol. 2016;34:973-81 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1000; loading ...; tbl 1
Ito M, Nakamura K, Mori F, Miki Y, Tanji K, Wakabayashi K. Novel eosinophilic neuronal cytoplasmic inclusions in the external cuneate nucleus of humans. Neuropathology. 2016;36:441-447 pubmed publisher
  • immunocytochemistry; rat; 1:600; fig 1
Rojas F, Gonzalez D, Cortes N, Ampuero E, Hernández D, Fritz E, et al. Reactive oxygen species trigger motoneuron death in non-cell-autonomous models of ALS through activation of c-Abl signaling. Front Cell Neurosci. 2015;9:203 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 3
Inoue K, Fujimura H, Ueda K, Matsumura T, Itoh K, Sakoda S. An autopsy case of neuronal intermediate filament inclusion disease with regard to immunophenotypic and topographical analysis of the neuronal inclusions. Neuropathology. 2015;35:545-52 pubmed publisher
  • immunohistochemistry - free floating section; mouse
Collins J, King A, Woodhouse A, Kirkcaldie M, Vickers J. The effect of focal brain injury on beta-amyloid plaque deposition, inflammation and synapses in the APP/PS1 mouse model of Alzheimer's disease. Exp Neurol. 2015;267:219-29 pubmed publisher
  • immunohistochemistry; mouse; 1:1000
Lee S, Meyer A, Schubert T, Hüser L, Dedek K, Haverkamp S. Morphology and connectivity of the small bistratified A8 amacrine cell in the mouse retina. J Comp Neurol. 2015;523:1529-47 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1000
Inose Y, Kato Y, Kitagawa K, Uchiyama S, Shibata N. Activated microglia in ischemic stroke penumbra upregulate MCP-1 and CCR2 expression in response to lysophosphatidylcholine derived from adjacent neurons and astrocytes. Neuropathology. 2015;35:209-23 pubmed publisher
  • immunohistochemistry; mouse; 1:500
Bernard Marissal N, Sunyach C, Marissal T, Raoul C, Pettmann B. Calreticulin levels determine onset of early muscle denervation by fast motoneurons of ALS model mice. Neurobiol Dis. 2015;73:130-6 pubmed publisher
  • immunohistochemistry - free floating section; mouse
  • immunohistochemistry - free floating section; rat
Broms J, Antolin Fontes B, Tingström A, Ibañez Tallon I. Conserved expression of the GPR151 receptor in habenular axonal projections of vertebrates. J Comp Neurol. 2015;523:359-80 pubmed publisher
  • western blot; mouse; 1:1000
Chittoor V, Sooyeon L, Rangaraju S, Nicks J, Schmidt J, Madorsky I, et al. Biochemical characterization of protein quality control mechanisms during disease progression in the C22 mouse model of CMT1A. ASN Neuro. 2013;5:e00128 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500
Puglisi F, Vanni V, Ponterio G, Tassone A, Sciamanna G, Bonsi P, et al. Torsin A Localization in the Mouse Cerebellar Synaptic Circuitry. PLoS ONE. 2013;8:e68063 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000
De la Huerta I, Kim I, Voinescu P, Sanes J. Direction-selective retinal ganglion cells arise from molecularly specified multipotential progenitors. Proc Natl Acad Sci U S A. 2012;109:17663-8 pubmed publisher
  • immunohistochemistry - free floating section; rat; 1:1000
Ingham E, Gunhan E, Fuller P, Fuller C. Immunotoxin-induced ablation of melanopsin retinal ganglion cells in a non-murine mammalian model. J Comp Neurol. 2009;516:125-40 pubmed publisher
  • immunohistochemistry - free floating section; rhesus macaque; 1:2,000
Lavenex P, Lavenex P, Bennett J, Amaral D. Postmortem changes in the neuroanatomical characteristics of the primate brain: hippocampal formation. J Comp Neurol. 2009;512:27-51 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:6,000
  • immunohistochemistry - frozen section; African green monkey; 1:1,000
Horn A, Eberhorn A, Hartig W, Ardeleanu P, Messoudi A, Büttner Ennever J. Perioculomotor cell groups in monkey and man defined by their histochemical and functional properties: reappraisal of the Edinger-Westphal nucleus. J Comp Neurol. 2008;507:1317-35 pubmed publisher
  • immunohistochemistry - free floating section; rhesus macaque
Saleem K, Price J, Hashikawa T. Cytoarchitectonic and chemoarchitectonic subdivisions of the perirhinal and parahippocampal cortices in macaque monkeys. J Comp Neurol. 2007;500:973-1006 pubmed
  • immunocytochemistry; rat; 1:2000
King A, Chung R, Vickers J, Dickson T. Localization of glutamate receptors in developing cortical neurons in culture and relationship to susceptibility to excitotoxicity. J Comp Neurol. 2006;498:277-94 pubmed
Weil T, Daly K, Yarur Castillo H, Thomsen M, Wang H, Mercau M, et al. Daily changes in light influence mood via inhibitory networks within the thalamic perihabenular nucleus. Sci Adv. 2022;8:eabn3567 pubmed publisher
Wolf C, Pouya A, Bitar S, Pfeiffer A, Bueno D, Rojas Charry L, et al. GDAP1 loss of function inhibits the mitochondrial pyruvate dehydrogenase complex by altering the actin cytoskeleton. Commun Biol. 2022;5:541 pubmed publisher
de Lamotte J, Polentes J, Roussange F, Lesueur L, Feurgard P, Perrier A, et al. Optogenetically controlled human functional motor endplate for testing botulinum neurotoxins. Stem Cell Res Ther. 2021;12:599 pubmed publisher
Waters S, Swanson M, Dieriks B, Zhang Y, Grimsey N, Murray H, et al. Blood-spinal cord barrier leakage is independent of motor neuron pathology in ALS. Acta Neuropathol Commun. 2021;9:144 pubmed publisher
Clark C, Clark R, Hoyle J, Chuckowree J, McLean C, Dickson T. Differential NPY-Y1 Receptor Density in the Motor Cortex of ALS Patients and Familial Model of ALS. Brain Sci. 2021;11: pubmed publisher
Shi L, Sun Z, Su W, Xu F, Xie D, Zhang Q, et al. Treg cell-derived osteopontin promotes microglia-mediated white matter repair after ischemic stroke. Immunity. 2021;54:1527-1542.e8 pubmed publisher
Wu Y, Wu H, Zeng J, Pluimer B, Dong S, Xie X, et al. Mild traumatic brain injury induces microvascular injury and accelerates Alzheimer-like pathogenesis in mice. Acta Neuropathol Commun. 2021;9:74 pubmed publisher
Roboon J, Hattori T, Ishii H, Takarada Iemata M, Nguyen D, Heer C, et al. Inhibition of CD38 and supplementation of nicotinamide riboside ameliorate lipopolysaccharide-induced microglial and astrocytic neuroinflammation by increasing NAD. J Neurochem. 2021;158:311-327 pubmed publisher
Calvo Barreiro L, Eixarch H, Cornejo T, Costa C, Castillo M, Mestre L, et al. Selected Clostridia Strains from The Human Microbiota and their Metabolite, Butyrate, Improve Experimental Autoimmune Encephalomyelitis. Neurotherapeutics. 2021;18:920-937 pubmed publisher
Wang F, Li E, De L, Wu Q, Zhang Y. OFF-transient alpha RGCs mediate looming triggered innate defensive response. Curr Biol. 2021;31:2263-2273.e3 pubmed publisher
Brodie Kommit J, Clark B, Shi Q, Shiau F, Kim D, Langel J, et al. Atoh7-independent specification of retinal ganglion cell identity. Sci Adv. 2021;7: pubmed publisher
Rosenkranz S, Shaposhnykov A, Träger S, Engler J, Witte M, Roth V, et al. Enhancing mitochondrial activity in neurons protects against neurodegeneration in a mouse model of multiple sclerosis. elife. 2021;10: pubmed publisher
Hughes R, Bosanac T, Mao X, Engber T, DiAntonio A, Milbrandt J, et al. Small Molecule SARM1 Inhibitors Recapitulate the SARM1-/- Phenotype and Allow Recovery of a Metastable Pool of Axons Fated to Degenerate. Cell Rep. 2021;34:108588 pubmed publisher
Ho R, Workman M, Mathkar P, Wu K, Kim K, O Rourke J, et al. Cross-Comparison of Human iPSC Motor Neuron Models of Familial and Sporadic ALS Reveals Early and Convergent Transcriptomic Disease Signatures. Cell Syst. 2021;12:159-175.e9 pubmed publisher
Zhang S, Wang Y, Xu J, Kim B, Deng W, Guo F. HIFα Regulates Developmental Myelination Independent of Autocrine Wnt Signaling. J Neurosci. 2021;41:251-268 pubmed publisher
Mikhalkin A, Nikitina N, Merkulyeva N. Heterochrony of postnatal accumulation of nonphosphorylated heavy-chain neurofilament by neurons of the cat dorsal lateral geniculate nucleus. J Comp Neurol. 2021;529:1430-1441 pubmed publisher
Lv Q, Yan M, Shen X, Wu J, Yu W, Yan S, et al. Normative Analysis of Individual Brain Differences Based on a Population MRI-Based Atlas of Cynomolgus Macaques. Cereb Cortex. 2021;31:341-355 pubmed publisher
Sepehrimanesh M, Ding B. Generation and optimization of highly pure motor neurons from human induced pluripotent stem cells via lentiviral delivery of transcription factors. Am J Physiol Cell Physiol. 2020;319:C771-C780 pubmed publisher
Richard P, Feng S, Tsai Y, Li W, Rinchetti P, Muhith U, et al. SETX (senataxin), the helicase mutated in AOA2 and ALS4, functions in autophagy regulation. Autophagy. 2020;: pubmed publisher
Sullivan G, Knutsen A, Peruzzotti Jametti L, Korotcov A, Bosomtwi A, Dardzinski B, et al. Transplantation of induced neural stem cells (iNSCs) into chronically demyelinated corpus callosum ameliorates motor deficits. Acta Neuropathol Commun. 2020;8:84 pubmed publisher
Jensen B, Schuldi M, McAvoy K, Russell K, Boehringer A, Curran B, et al. Synaptic dysfunction induced by glycine-alanine dipeptides in C9orf72-ALS/FTD is rescued by SV2 replenishment. EMBO Mol Med. 2020;12:e10722 pubmed publisher
Berger S, Stattmann M, Cicvaric A, Monje F, Coiro P, Hotka M, et al. Severe hydroxymethylbilane synthase deficiency causes depression-like behavior and mitochondrial dysfunction in a mouse model of homozygous dominant acute intermittent porphyria. Acta Neuropathol Commun. 2020;8:38 pubmed publisher
Glaß H, Neumann P, Pal A, Reinhardt P, Storch A, Sterneckert J, et al. Combined Dendritic and Axonal Deterioration Are Responsible for Motoneuronopathy in Patient-Derived Neuronal Cell Models of Chorea-Acanthocytosis. Int J Mol Sci. 2020;21: pubmed publisher
Abo Rady M, Kalmbach N, Pal A, Schludi C, Janosch A, Richter T, et al. Knocking out C9ORF72 Exacerbates Axonal Trafficking Defects Associated with Hexanucleotide Repeat Expansion and Reduces Levels of Heat Shock Proteins. Stem Cell Reports. 2020;14:390-405 pubmed publisher
Puller C, Duda S, Lotfi E, Arzhangnia Y, Block C, Ahlers M, et al. Electrical Coupling of Heterotypic Ganglion Cells in the Mammalian Retina. J Neurosci. 2020;40:1302-1310 pubmed publisher
Sen T, Saha P, Gupta R, Foley L, Jiang T, Abakumova O, et al. Aberrant ER Stress Induced Neuronal-IFNβ Elicits White Matter Injury Due to Microglial Activation and T-Cell Infiltration after TBI. J Neurosci. 2020;40:424-446 pubmed publisher
Tan H, Chen M, Pang D, Xia X, Du C, Yang W, et al. LanCL1 promotes motor neuron survival and extends the lifespan of amyotrophic lateral sclerosis mice. Cell Death Differ. 2020;27:1369-1382 pubmed publisher
Drohomyrecky P, Doroshenko E, Akkermann R, Moshkova M, Yi T, Zhao F, et al. Peroxisome Proliferator-Activated Receptor-δ Acts within Peripheral Myeloid Cells to Limit Th Cell Priming during Experimental Autoimmune Encephalomyelitis. J Immunol. 2019;203:2588-2601 pubmed publisher
Iida M, Sahashi K, Kondo N, Nakatsuji H, Tohnai G, Tsutsumi Y, et al. Src inhibition attenuates polyglutamine-mediated neuromuscular degeneration in spinal and bulbar muscular atrophy. Nat Commun. 2019;10:4262 pubmed publisher
Laug D, Huang T, Huerta N, Huang A, Sardar D, Ortiz Guzman J, et al. Nuclear factor I-A regulates diverse reactive astrocyte responses after CNS injury. J Clin Invest. 2019;129:4408-4418 pubmed publisher
Kinoshita H, Maki T, Yasuda K, Kishida N, Sasaoka N, Takagi Y, et al. KUS121, a valosin-containing protein modulator, attenuates ischemic stroke via preventing ATP depletion. Sci Rep. 2019;9:11519 pubmed publisher
Jin J, Smith M, Kersbergen C, Kam T, Viswanathan M, Martin K, et al. Glial pathology and retinal neurotoxicity in the anterior visual pathway in experimental autoimmune encephalomyelitis. Acta Neuropathol Commun. 2019;7:125 pubmed publisher
Schirmer L, Velmeshev D, Holmqvist S, Kaufmann M, Werneburg S, Jung D, et al. Neuronal vulnerability and multilineage diversity in multiple sclerosis. Nature. 2019;573:75-82 pubmed publisher
Roboon J, Hattori T, Ishii H, Takarada Iemata M, Le T, Shiraishi Y, et al. Deletion of CD38 Suppresses Glial Activation and Neuroinflammation in a Mouse Model of Demyelination. Front Cell Neurosci. 2019;13:258 pubmed publisher
Tung Y, Peng K, Chen Y, Yen Y, Chang M, Thams S, et al. Mir-17∼92 Confers Motor Neuron Subtype Differential Resistance to ALS-Associated Degeneration. Cell Stem Cell. 2019;: pubmed publisher
Reinehr S, Reinhard J, Wiemann S, Hesse K, Voss C, Gandej M, et al. Transfer of the Experimental Autoimmune Glaucoma Model from Rats to Mice-New Options to Study Glaucoma Disease. Int J Mol Sci. 2019;20: pubmed publisher
Jiang L, Zhu B, Zhao Y, Li X, Liu T, Piña Crespo J, et al. Membralin deficiency dysregulates astrocytic glutamate homeostasis leading to ALS-like impairment. J Clin Invest. 2019;130: pubmed publisher
Dyer M, Phipps A, Mitew S, Taberlay P, Woodhouse A. Age, but Not Amyloidosis, Induced Changes in Global Levels of Histone Modifications in Susceptible and Disease-Resistant Neurons in Alzheimer's Disease Model Mice. Front Aging Neurosci. 2019;11:68 pubmed publisher
Salib M, Joshi A, Katona L, Howarth M, Micklem B, Somogyi P, et al. GABAergic Medial Septal Neurons with Low-Rhythmic Firing Innervating the Dentate Gyrus and Hippocampal Area CA3. J Neurosci. 2019;39:4527-4549 pubmed publisher
Zhou Y, Carmona S, Muhammad A, Bell S, Landeros J, Vazquez M, et al. Restoring mitofusin balance prevents axonal degeneration in a Charcot-Marie-Tooth type 2A model. J Clin Invest. 2019;130:1756-1771 pubmed publisher
Keskin I, Forsgren E, Lehmann M, Andersen P, Brannstrom T, Lange D, et al. The molecular pathogenesis of superoxide dismutase 1-linked ALS is promoted by low oxygen tension. Acta Neuropathol. 2019;138:85-101 pubmed publisher
McLeod V, Lau C, Chiam M, Rupasinghe T, Roessner U, Djouma E, et al. Androgen receptor antagonism accelerates disease onset in the SOD1G93A mouse model of amyotrophic lateral sclerosis. Br J Pharmacol. 2019;176:2111-2130 pubmed publisher
McWilliams I, Kielczewski J, Ireland D, Sykes J, Lewkowicz A, Konduru K, et al. Pseudovirus rVSVΔG-ZEBOV-GP Infects Neurons in Retina and CNS, Causing Apoptosis and Neurodegeneration in Neonatal Mice. Cell Rep. 2019;26:1718-1726.e4 pubmed publisher
Wong R, Lénárt N, Hill L, Toms L, Coutts G, Martinecz B, et al. Interleukin-1 mediates ischaemic brain injury via distinct actions on endothelial cells and cholinergic neurons. Brain Behav Immun. 2019;76:126-138 pubmed publisher
Contestabile A, Colangiulo R, Lucchini M, Gindrat A, Hamadjida A, Kaeser M, et al. Asymmetric and Distant Effects of a Unilateral Lesion of the Primary Motor Cortex on the Bilateral Supplementary Motor Areas in Adult Macaque Monkeys. J Neurosci. 2018;38:10644-10656 pubmed publisher
López Erauskin J, Tadokoro T, Baughn M, Myers B, McAlonis Downes M, Chillon Marinas C, et al. ALS/FTD-Linked Mutation in FUS Suppresses Intra-axonal Protein Synthesis and Drives Disease Without Nuclear Loss-of-Function of FUS. Neuron. 2018;100:816-830.e7 pubmed publisher
Shorrock H, van der Hoorn D, Boyd P, Llavero Hurtado M, Lamont D, Wirth B, et al. UBA1/GARS-dependent pathways drive sensory-motor connectivity defects in spinal muscular atrophy. Brain. 2018;141:2878-2894 pubmed publisher
Schellino R, Boido M, Borsello T, Vercelli A. Pharmacological c-Jun NH2-Terminal Kinase (JNK) Pathway Inhibition Reduces Severity of Spinal Muscular Atrophy Disease in Mice. Front Mol Neurosci. 2018;11:308 pubmed publisher
Rahim T, Becquart P, Baeva M, Quandt J. Expression of the neuroprotective protein aryl hydrocarbon receptor nuclear translocator 2 correlates with neuronal stress and disability in models of multiple sclerosis. J Neuroinflammation. 2018;15:270 pubmed publisher
Alpar A, Zahola P, Hanics J, Hevesi Z, Korchynska S, Benevento M, et al. Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress. EMBO J. 2018;37: pubmed publisher
Ayhan F, Perez B, Shorrock H, Zu T, Bañez Coronel M, Reid T, et al. SCA8 RAN polySer protein preferentially accumulates in white matter regions and is regulated by eIF3F. EMBO J. 2018;37: pubmed publisher
Xia Y, Pu H, Leak R, Shi Y, Mu H, Hu X, et al. Tissue plasminogen activator promotes white matter integrity and functional recovery in a murine model of traumatic brain injury. Proc Natl Acad Sci U S A. 2018;115:E9230-E9238 pubmed publisher
Nardo G, Trolese M, Verderio M, Mariani A, De Paola M, Riva N, et al. Counteracting roles of MHCI and CD8+ T cells in the peripheral and central nervous system of ALS SOD1G93A mice. Mol Neurodegener. 2018;13:42 pubmed publisher
Maruyama T, Baba T, Maemoto Y, Hara Miyauchi C, Hasegawa Ogawa M, Okano H, et al. Loss of DDHD2, whose mutation causes spastic paraplegia, promotes reactive oxygen species generation and apoptosis. Cell Death Dis. 2018;9:797 pubmed publisher
Sonoda T, Lee S, Birnbaumer L, Schmidt T. Melanopsin Phototransduction Is Repurposed by ipRGC Subtypes to Shape the Function of Distinct Visual Circuits. Neuron. 2018;99:754-767.e4 pubmed publisher
Greco V, Spalloni A, Corasolla Carregari V, Pieroni L, Persichilli S, Mercuri N, et al. Proteomics and Toxicity Analysis of Spinal-Cord Primary Cultures upon Hydrogen Sulfide Treatment. Antioxidants (Basel). 2018;7: pubmed publisher
Cocozza G, Di Castro M, Carbonari L, Grimaldi A, Antonangeli F, Garofalo S, et al. Ca2+-activated K+ channels modulate microglia affecting motor neuron survival in hSOD1G93A mice. Brain Behav Immun. 2018;73:584-595 pubmed publisher
Maciel R, Bis D, Rebelo A, Saghira C, Zuchner S, Saporta M. The human motor neuron axonal transcriptome is enriched for transcripts related to mitochondrial function and microtubule-based axonal transport. Exp Neurol. 2018;307:155-163 pubmed publisher
Cignarella F, Cantoni C, Ghezzi L, Salter A, Dorsett Y, Chen L, et al. Intermittent Fasting Confers Protection in CNS Autoimmunity by Altering the Gut Microbiota. Cell Metab. 2018;27:1222-1235.e6 pubmed publisher
Hirono M, Watanabe S, Karube F, Fujiyama F, Kawahara S, Nagao S, et al. Perineuronal Nets in the Deep Cerebellar Nuclei Regulate GABAergic Transmission and Delay Eyeblink Conditioning. J Neurosci. 2018;38:6130-6144 pubmed publisher
Bailey R, Armao D, Nagabhushan Kalburgi S, Gray S. Development of Intrathecal AAV9 Gene Therapy for Giant Axonal Neuropathy. Mol Ther Methods Clin Dev. 2018;9:160-171 pubmed publisher
Romeo Guitart D, Leiva Rodríguez T, Espinosa Alcantud M, Sima N, Vaquero A, Domínguez Martín H, et al. SIRT1 activation with neuroheal is neuroprotective but SIRT2 inhibition with AK7 is detrimental for disconnected motoneurons. Cell Death Dis. 2018;9:531 pubmed publisher
De Pace R, Skirzewski M, Damme M, Mattera R, Mercurio J, Foster A, et al. Altered distribution of ATG9A and accumulation of axonal aggregates in neurons from a mouse model of AP-4 deficiency syndrome. PLoS Genet. 2018;14:e1007363 pubmed publisher
Butler B, Sunstrum J, Lomber S. Modified Origins of Cortical Projections to the Superior Colliculus in the Deaf: Dispersion of Auditory Efferents. J Neurosci. 2018;38:4048-4058 pubmed publisher
Kelley K, Ben Haim L, Schirmer L, Tyzack G, Tolman M, Miller J, et al. Kir4.1-Dependent Astrocyte-Fast Motor Neuron Interactions Are Required for Peak Strength. Neuron. 2018;98:306-319.e7 pubmed publisher
Larson V, Mironova Y, Vanderpool K, Waisman A, Rash J, Agarwal A, et al. Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility. elife. 2018;7: pubmed publisher
Risner M, Pasini S, Cooper M, Lambert W, Calkins D. Axogenic mechanism enhances retinal ganglion cell excitability during early progression in glaucoma. Proc Natl Acad Sci U S A. 2018;115:E2393-E2402 pubmed publisher
Muñoz S, Li H, Ruberu K, Chu Q, Saghatelian A, Ooi L, et al. The serine protease HtrA1 contributes to the formation of an extracellular 25-kDa apolipoprotein E fragment that stimulates neuritogenesis. J Biol Chem. 2018;293:4071-4084 pubmed publisher
Naumann M, Pal A, Goswami A, Lojewski X, Japtok J, Vehlow A, et al. Impaired DNA damage response signaling by FUS-NLS mutations leads to neurodegeneration and FUS aggregate formation. Nat Commun. 2018;9:335 pubmed publisher
Joyce M, Barbas H. Cortical Connections Position Primate Area 25 as a Keystone for Interoception, Emotion, and Memory. J Neurosci. 2018;38:1677-1698 pubmed publisher
Weng Y, Wang X, An R, Cassin J, Vissers C, Liu Y, et al. Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System. Neuron. 2018;97:313-325.e6 pubmed publisher
Ito K, Ohkawara B, Yagi H, Nakashima H, Tsushima M, Ota K, et al. Lack of Fgf18 causes abnormal clustering of motor nerve terminals at the neuromuscular junction with reduced acetylcholine receptor clusters. Sci Rep. 2018;8:434 pubmed publisher
Takakura K, Ito S, Sonoda J, Tabata K, Shiozaki M, Nagai K, et al. Cordyceps militaris improves the survival of Dahl salt-sensitive hypertensive rats possibly via influences of mitochondria and autophagy functions. Heliyon. 2017;3:e00462 pubmed publisher
Griggs W, Kim H, Ghazizadeh A, Gabriela Costello M, Wall K, Hikosaka O. Flexible and Stable Value Coding Areas in Caudate Head and Tail Receive Anatomically Distinct Cortical and Subcortical Inputs. Front Neuroanat. 2017;11:106 pubmed publisher
Litvina E, Chen C. Functional Convergence at the Retinogeniculate Synapse. Neuron. 2017;96:330-338.e5 pubmed publisher
Abernathy D, Kim W, McCoy M, Lake A, Ouwenga R, Lee S, et al. MicroRNAs Induce a Permissive Chromatin Environment that Enables Neuronal Subtype-Specific Reprogramming of Adult Human Fibroblasts. Cell Stem Cell. 2017;21:332-348.e9 pubmed publisher
Penndorf D, Tadic V, Witte O, Grosskreutz J, Kretz A. DNA strand breaks and TDP-43 mislocation are absent in the murine hSOD1G93A model of amyotrophic lateral sclerosis in vivo and in vitro. PLoS ONE. 2017;12:e0183684 pubmed publisher
Krieger B, Qiao M, Rousso D, Sanes J, Meister M. Four alpha ganglion cell types in mouse retina: Function, structure, and molecular signatures. PLoS ONE. 2017;12:e0180091 pubmed publisher
Norante R, Massimino M, Lorenzon P, De Mario A, Peggion C, Vicario M, et al. Generation and validation of novel adeno-associated viral vectors for the analysis of Ca2+ homeostasis in motor neurons. Sci Rep. 2017;7:6521 pubmed publisher
Jacquier A, Delorme C, Belotti E, Juntas Morales R, Solé G, Dubourg O, et al. Cryptic amyloidogenic elements in mutant NEFH causing Charcot-Marie-Tooth 2 trigger aggresome formation and neuronal death. Acta Neuropathol Commun. 2017;5:55 pubmed publisher
Cumberworth S, Barrie J, Cunningham M, de Figueiredo D, Schultz V, Wilder Smith A, et al. Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected. Acta Neuropathol Commun. 2017;5:50 pubmed publisher
Logan A, Mammel A, Robinson D, Chin A, Condon A, Robinson F. Schwann cell-specific deletion of the endosomal PI 3-kinase Vps34 leads to delayed radial sorting of axons, arrested myelination, and abnormal ErbB2-ErbB3 tyrosine kinase signaling. Glia. 2017;65:1452-1470 pubmed publisher
Corsini S, Tortora M, Rauti R, Nistri A. Nicotine protects rat hypoglossal motoneurons from excitotoxic death via downregulation of connexin 36. Cell Death Dis. 2017;8:e2881 pubmed publisher
Akopian A, Kumar S, Ramakrishnan H, Roy K, Viswanathan S, Bloomfield S. Targeting neuronal gap junctions in mouse retina offers neuroprotection in glaucoma. J Clin Invest. 2017;127:2647-2661 pubmed publisher
Schultz V, van der Meer F, Wrzos C, Scheidt U, Bahn E, Stadelmann C, et al. Acutely damaged axons are remyelinated in multiple sclerosis and experimental models of demyelination. Glia. 2017;65:1350-1360 pubmed publisher
Llorens F, Thüne K, Sikorska B, Schmitz M, Tahir W, Fernández Borges N, et al. Altered Ca2+ homeostasis induces Calpain-Cathepsin axis activation in sporadic Creutzfeldt-Jakob disease. Acta Neuropathol Commun. 2017;5:35 pubmed publisher
Himmelein S, Lindemann A, Sinicina I, Horn A, Brandt T, Strupp M, et al. Differential Involvement during Latent Herpes Simplex Virus 1 Infection of the Superior and Inferior Divisions of the Vestibular Ganglia: Implications for Vestibular Neuritis. J Virol. 2017;91: pubmed publisher
Karamita M, Barnum C, Mobius W, TANSEY M, Szymkowski D, Lassmann H, et al. Therapeutic inhibition of soluble brain TNF promotes remyelination by increasing myelin phagocytosis by microglia. JCI Insight. 2017;2: pubmed publisher
Sun D, Moore S, Jakobs T. Optic nerve astrocyte reactivity protects function in experimental glaucoma and other nerve injuries. J Exp Med. 2017;214:1411-1430 pubmed publisher
Hoye M, Koval E, Wegener A, Hyman T, Yang C, O Brien D, et al. MicroRNA Profiling Reveals Marker of Motor Neuron Disease in ALS Models. J Neurosci. 2017;37:5574-5586 pubmed publisher
Woo C, Maier V, Davey R, Brennan J, Li G, Brothers J, et al. Gene activation of SMN by selective disruption of lncRNA-mediated recruitment of PRC2 for the treatment of spinal muscular atrophy. Proc Natl Acad Sci U S A. 2017;114:E1509-E1518 pubmed publisher
Li X, Wang Z, Tan L, Wang Y, Lu C, Chen R, et al. Correcting miR92a-vGAT-Mediated GABAergic Dysfunctions Rescues Human Tau-Induced Anxiety in Mice. Mol Ther. 2017;25:140-152 pubmed publisher
Lanz T, Williams S, Stojic A, Iwantscheff S, Sonner J, Grabitz C, et al. Tryptophan-2,3-Dioxygenase (TDO) deficiency is associated with subclinical neuroprotection in a mouse model of multiple sclerosis. Sci Rep. 2017;7:41271 pubmed publisher
Bukreeva I, Campi G, Fratini M, Spano R, Bucci D, Battaglia G, et al. Quantitative 3D investigation of Neuronal network in mouse spinal cord model. Sci Rep. 2017;7:41054 pubmed publisher
Feng L, Puyang Z, Chen H, Liang P, Troy J, Liu X. Overexpression of Brain-Derived Neurotrophic Factor Protects Large Retinal Ganglion Cells After Optic Nerve Crush in Mice. Eneuro. 2017;4: pubmed publisher
Wood K, Town S, Atilgan H, Jones G, Bizley J. Acute Inactivation of Primary Auditory Cortex Causes a Sound Localisation Deficit in Ferrets. PLoS ONE. 2017;12:e0170264 pubmed publisher
Petrozziello T, Secondo A, Tedeschi V, Esposito A, Sisalli M, Scorziello A, et al. ApoSOD1 lacking dismutase activity neuroprotects motor neurons exposed to beta-methylamino-L-alanine through the Ca2+/Akt/ERK1/2 prosurvival pathway. Cell Death Differ. 2017;24:511-522 pubmed publisher
Casanovas A, Salvany S, Lahoz V, Tarabal O, Piedrafita L, Sabater R, et al. Neuregulin 1-ErbB module in C-bouton synapses on somatic motor neurons: molecular compartmentation and response to peripheral nerve injury. Sci Rep. 2017;7:40155 pubmed publisher
Toonen J, Solga A, Ma Y, Gutmann D. Estrogen activation of microglia underlies the sexually dimorphic differences in Nf1 optic glioma-induced retinal pathology. J Exp Med. 2017;214:17-25 pubmed publisher
Scholl T, Mühlebner A, Ricken G, Gruber V, Fabing A, Samueli S, et al. Impaired oligodendroglial turnover is associated with myelin pathology in focal cortical dysplasia and tuberous sclerosis complex. Brain Pathol. 2017;27:770-780 pubmed publisher
Ou Y, Jo R, Ullian E, Wong R, Della Santina L. Selective Vulnerability of Specific Retinal Ganglion Cell Types and Synapses after Transient Ocular Hypertension. J Neurosci. 2016;36:9240-52 pubmed publisher
Rizzo F, Ronchi D, Salani S, Nizzardo M, Fortunato F, Bordoni A, et al. Selective mitochondrial depletion, apoptosis resistance, and increased mitophagy in human Charcot-Marie-Tooth 2A motor neurons. Hum Mol Genet. 2016;25:4266-4281 pubmed publisher
Ho R, Sances S, Gowing G, Amoroso M, O Rourke J, Sahabian A, et al. ALS disrupts spinal motor neuron maturation and aging pathways within gene co-expression networks. Nat Neurosci. 2016;19:1256-67 pubmed publisher
Azeez I, Olopade F, Laperchia C, Andrioli A, Scambi I, Onwuka S, et al. Regional Myelin and Axon Damage and Neuroinflammation in the Adult Mouse Brain After Long-Term Postnatal Vanadium Exposure. J Neuropathol Exp Neurol. 2016;75:843-54 pubmed publisher
Yoo M, Kim T, Yoon Y, Koh J. Autism phenotypes in ZnT3 null mice: Involvement of zinc dyshomeostasis, MMP-9 activation and BDNF upregulation. Sci Rep. 2016;6:28548 pubmed publisher
Brambilla L, Guidotti G, Martorana F, Iyer A, Aronica E, Valori C, et al. Disruption of the astrocytic TNFR1-GDNF axis accelerates motor neuron degeneration and disease progression in amyotrophic lateral sclerosis. Hum Mol Genet. 2016;25:3080-3095 pubmed
Luna G, Keeley P, Reese B, Linberg K, Lewis G, Fisher S. Astrocyte structural reactivity and plasticity in models of retinal detachment. Exp Eye Res. 2016;150:4-21 pubmed publisher
Körner S, Böselt S, Wichmann K, Thau Habermann N, Zapf A, Knippenberg S, et al. The Axon Guidance Protein Semaphorin 3A Is Increased in the Motor Cortex of Patients With Amyotrophic Lateral Sclerosis. J Neuropathol Exp Neurol. 2016;: pubmed
Qu C, Bian D, Li X, Xiao J, Wu C, Li Y, et al. Transient Expression of Fez Family Zinc Finger 2 Protein Regulates the Brn3b Gene in Developing Retinal Ganglion Cells. J Biol Chem. 2016;291:7661-8 pubmed publisher
Li S, Yang C, Zhang L, Gao X, Wang X, Liu W, et al. Promoting axon regeneration in the adult CNS by modulation of the melanopsin/GPCR signaling. Proc Natl Acad Sci U S A. 2016;113:1937-42 pubmed publisher
Donkels C, Pfeifer D, Janz P, Huber S, Nakagawa J, Prinz M, et al. Whole Transcriptome Screening Reveals Myelination Deficits in Dysplastic Human Temporal Neocortex. Cereb Cortex. 2017;27:1558-1572 pubmed publisher
Parisi C, Napoli G, Amadio S, Spalloni A, Apolloni S, Longone P, et al. MicroRNA-125b regulates microglia activation and motor neuron death in ALS. Cell Death Differ. 2016;23:531-41 pubmed publisher
Patitucci T, Ebert A. SMN deficiency does not induce oxidative stress in SMA iPSC-derived astrocytes or motor neurons. Hum Mol Genet. 2016;25:514-23 pubmed publisher
Berry R, Qu J, John S, Howell G, Jakobs T. Synapse Loss and Dendrite Remodeling in a Mouse Model of Glaucoma. PLoS ONE. 2015;10:e0144341 pubmed publisher
Wang H, Hsieh P, Huang D, Chin P, Chou C, Tung C, et al. RBFOX3/NeuN is Required for Hippocampal Circuit Balance and Function. Sci Rep. 2015;5:17383 pubmed publisher
Wagener R, Witte M, Guy J, Mingo Moreno N, Kügler S, Staiger J. Thalamocortical Connections Drive Intracortical Activation of Functional Columns in the Mislaminated Reeler Somatosensory Cortex. Cereb Cortex. 2016;26:820-37 pubmed publisher
MacNair L, Xiao S, Miletic D, Ghani M, Julien J, Keith J, et al. MTHFSD and DDX58 are novel RNA-binding proteins abnormally regulated in amyotrophic lateral sclerosis. Brain. 2016;139:86-100 pubmed publisher
Pagliarini V, Pelosi L, Bustamante M, Nobili A, Berardinelli M, D Amelio M, et al. SAM68 is a physiological regulator of SMN2 splicing in spinal muscular atrophy. J Cell Biol. 2015;211:77-90 pubmed publisher
Turner M, Pang P, Chretien N, Havari E, Lamorte M, Oliver J, et al. Reduction of inflammation and preservation of neurological function by anti-CD52 therapy in murine experimental autoimmune encephalomyelitis. J Neuroimmunol. 2015;285:4-12 pubmed publisher
Saba L, Viscomi M, Caioli S, Pignataro A, Bisicchia E, Pieri M, et al. Altered Functionality, Morphology, and Vesicular Glutamate Transporter Expression of Cortical Motor Neurons from a Presymptomatic Mouse Model of Amyotrophic Lateral Sclerosis. Cereb Cortex. 2016;26:1512-28 pubmed publisher
Redondo J, Kemp K, Hares K, Rice C, Scolding N, Wilkins A. Purkinje Cell Pathology and Loss in Multiple Sclerosis Cerebellum. Brain Pathol. 2015;25:692-700 pubmed publisher
Song L, Chen L, Zhang X, Li J, Le W. Resveratrol ameliorates motor neuron degeneration and improves survival in SOD1(G93A) mouse model of amyotrophic lateral sclerosis. Biomed Res Int. 2014;2014:483501 pubmed publisher
Kang S, Li Y, Fukaya M, Lorenzini I, Cleveland D, Ostrow L, et al. Degeneration and impaired regeneration of gray matter oligodendrocytes in amyotrophic lateral sclerosis. Nat Neurosci. 2013;16:571-9 pubmed publisher
Chang Q, Martin L. Glycine receptor channels in spinal motoneurons are abnormal in a transgenic mouse model of amyotrophic lateral sclerosis. J Neurosci. 2011;31:2815-27 pubmed publisher
Petzold A, Tisdall M, Girbes A, Martinian L, Thom M, Kitchen N, et al. In vivo monitoring of neuronal loss in traumatic brain injury: a microdialysis study. Brain. 2011;134:464-83 pubmed publisher
Kiryu Seo S, Ohno N, Kidd G, Komuro H, Trapp B. Demyelination increases axonal stationary mitochondrial size and the speed of axonal mitochondrial transport. J Neurosci. 2010;30:6658-66 pubmed publisher
Stevens H, Smith K, Maragnoli M, Fagel D, Borok E, Shanabrough M, et al. Fgfr2 is required for the development of the medial prefrontal cortex and its connections with limbic circuits. J Neurosci. 2010;30:5590-602 pubmed publisher
Howell G, Libby R, Jakobs T, Smith R, Phalan F, Barter J, et al. Axons of retinal ganglion cells are insulted in the optic nerve early in DBA/2J glaucoma. J Cell Biol. 2007;179:1523-37 pubmed
Maurel P, Einheber S, Galinska J, Thaker P, Lam I, Rubin M, et al. Nectin-like proteins mediate axon Schwann cell interactions along the internode and are essential for myelination. J Cell Biol. 2007;178:861-74 pubmed
Jakobs T, Libby R, Ben Y, John S, Masland R. Retinal ganglion cell degeneration is topological but not cell type specific in DBA/2J mice. J Cell Biol. 2005;171:313-25 pubmed
Xia C, Roberts E, Her L, Liu X, Williams D, Cleveland D, et al. Abnormal neurofilament transport caused by targeted disruption of neuronal kinesin heavy chain KIF5A. J Cell Biol. 2003;161:55-66 pubmed
Pot C, Simonen M, Weinmann O, Schnell L, Christ F, Stoeckle S, et al. Nogo-A expressed in Schwann cells impairs axonal regeneration after peripheral nerve injury. J Cell Biol. 2002;159:29-35 pubmed
Tomishima M, Enquist L. A conserved alpha-herpesvirus protein necessary for axonal localization of viral membrane proteins. J Cell Biol. 2001;154:741-52 pubmed
Sanchez I, Hassinger L, Sihag R, Cleveland D, Mohan P, Nixon R. Local control of neurofilament accumulation during radial growth of myelinating axons in vivo. Selective role of site-specific phosphorylation. J Cell Biol. 2000;151:1013-24 pubmed
product information
Antigen :
Neurofilament H NF-H
Apps. Abbrev. :
IHC-P, Western Blotting, Array Tomography, ICC
Cat # :
801701
Clone :
SMI 32
Item :
Purified (azide-free) anti-Neurofilament H (NF-H), Nonphosphorylated
Isotype :
Mouse IgG1
Other Names :
Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
Size :
100 μL
Price (USD) :
259 USD
Reactivity :
Human, Mouse, Rat
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PUREAF
Application Notes :
Additional reported applications (for the relevant formats) include Western blotting 6 , immunohistochemistry 4,5 , immunocytochemistry 1,2,3, 7 , array tomography 8 . Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house 4 . This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca 2+ uptake.
more info or order :
company information
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