Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

Alexa Fluor® 488 anti-Neurofilament H (NF-H), Phosphorylated

801611

25 μg

129 USD

monoclonal

mouse

AF488

SMI 31

human, mouse, rat

IHC-P

801611

SMI 31

Alexa Fluor® 488 anti-Neurofilament H (NF-H), Phosphorylated

Mouse IgG1, κ

Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein

25 μg

129 USD

Human, Mouse, Rat

Monoclonal

Mouse

A488

Additional reported applications (for the relevant formats) include: Western blotting^1, immunohistochemistry^2,4, and immunocytochemistry^4.SMI 31 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, which chicken and frog (Xenopus). Immunocytochemically, SMI 31 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 31. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 31 may be found also in neuronal cell bodies. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures with SMI 31 by agents that induce stress-activated protein kinase. In its reaction with paired helical filaments in hereditary inclusion body myopathy, SMI 31 colocalizes with nitric oxide synthase, suggesting that oxidative stress may play a role in the pathogenic cascade of such degenerative diseases. SMI 31 co-immunoprecipitates neurofilament-associated kinase (NAK 115) via reaction of the antibody with the tail domain of neurofilament H.;