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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-Neurofilament H (NF-H), Phosphorylated
catalog :
801602
quantity :
25 μL
price :
112 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SMI 31
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 80
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; human; loading ...; fig s17a
Halabi E, Pinotsi D, Rivera Fuentes P. Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching. Nat Commun. 2019;10:1232 pubmed publisher
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D Mello V, Subramaniam M, Bhalla A, Saavedra S, Leiba O, Levison S. Intranasal Leukemia Inhibitory Factor Attenuates Gliosis and Axonal Injury and Improves Sensorimotor Function After a Mild Pediatric Traumatic Brain Injury. Neurotrauma Rep. 2023;4:236-250 pubmed publisher
Seiler S, W xe4 lti C, de Barros V, Barbash S, Foo L. Higher throughput workflow with sensitive, reliable and automatic quantification of myelination in vitro suitable for drug screening. Sci Rep. 2023;13:2883 pubmed publisher
Hu T, Meng S, Zhang Q, Song S, Tan C, Huang J, et al. Astrocyte derived TSP2 contributes to synaptic alteration and visual dysfunction in retinal ischemia/reperfusion injury. Cell Biosci. 2022;12:196 pubmed publisher
xd6 zen I, Mai H, De Maio A, Ruscher K, Michalettos G, Clausen F, et al. Purkinje cell vulnerability induced by diffuse traumatic brain injury is linked to disruption of long-range neuronal circuits. Acta Neuropathol Commun. 2022;10:129 pubmed publisher
Xu X, Beleza R, Gon xe7 alves F, Valbuena S, Al xe7 ada Morais S, Gon xe7 alves N, et al. Adenosine A2A receptors control synaptic remodeling in the adult brain. Sci Rep. 2022;12:14690 pubmed publisher
Manangeeswaran M, Ireland D, Thacker S, Lee H, Kelley Baker L, Lewkowicz A, et al. BSL2-compliant lethal mouse model of SARS-CoV-2 and variants of concern to evaluate therapeutics targeting the Spike protein. Front Immunol. 2022;13:919815 pubmed publisher
Wang W, Liu Z, Jing B, Mai H, Jiao H, Guan T, et al. 4,8-dicarboxyl-8,9-iridoid-1-glycoside Promotes Neural Stem Cell Differentiation Through MeCP2. Dose Response. 2022;20:15593258221112959 pubmed publisher
Won D, Kim J, Choi J, Kim H, Han S, Ha I, et al. Digital selective transformation and patterning of highly conductive hydrogel bioelectronics by laser-induced phase separation. Sci Adv. 2022;8:eabo3209 pubmed publisher
McGonigal R, Campbell C, Barrie J, Yao D, Cunningham M, Crawford C, et al. Schwann cell nodal membrane disruption triggers bystander axonal degeneration in a Guillain-Barré syndrome mouse model. J Clin Invest. 2022;132: pubmed publisher
Cunningham M, McGonigal R, Barrie J, Yao D, Willison H. Real time imaging of intra-axonal calcium flux in an explant mouse model of axonal Guillain-Barré syndrome. Exp Neurol. 2022;355:114127 pubmed publisher
Powers R, Daza R, Koehler A, Courchet J, Calabrese B, Hevner R, et al. Growth cone macropinocytosis of neurotrophin receptor and neuritogenesis are regulated by neuron navigator 1. Mol Biol Cell. 2022;33:ar64 pubmed publisher
Zhu P, Hung H, Batchenkova N, Nixon Abell J, Henderson J, Zheng P, et al. Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons. Hum Mol Genet. 2022;31:2779-2795 pubmed publisher
Pellegatta M, Canevazzi P, Forese M, Podini P, Valenzano S, Del Carro U, et al. ADAM17 Regulates p75NTR-Mediated Fibrinolysis and Nerve Remyelination. J Neurosci. 2022;42:2433-2447 pubmed publisher
Bishop R, Won S, Irvine K, Basu J, Rome E, Swanson R. Blast-induced axonal degeneration in the rat cerebellum in the absence of head movement. Sci Rep. 2022;12:143 pubmed publisher
Scaramuzzino C, Cuoc E, Pla P, Humbert S, Saudou F. Calcineurin and huntingtin form a calcium-sensing machinery that directs neurotrophic signals to the nucleus. Sci Adv. 2022;8:eabj8812 pubmed publisher
Frondelli M, Mather M, Levison S. Oligodendrocyte progenitor proliferation is disinhibited following traumatic brain injury in leukemia inhibitory factor heterozygous mice. J Neurosci Res. 2022;100:578-597 pubmed publisher
Chaves R, Tran M, Holder A, Balcer A, Dickey A, Roberts E, et al. Amyloidogenic Processing of Amyloid Precursor Protein Drives Stretch-Induced Disruption of Axonal Transport in hiPSC-Derived Neurons. J Neurosci. 2021;41:10034-10053 pubmed publisher
Al xe7 ada Morais S, Gon xe7 alves N, Moreno Juan V, Andres B, Ferreira S, Marques J, et al. Adenosine A2A Receptors Contribute to the Radial Migration of Cortical Projection Neurons through the Regulation of Neuronal Polarization and Axon Formation. Cereb Cortex. 2021;31:5652-5663 pubmed publisher
Johari M, Sarparanta J, Vihola A, Jonson P, Savarese M, Jokela M, et al. Missense mutations in small muscle protein X-linked (SMPX) cause distal myopathy with protein inclusions. Acta Neuropathol. 2021;142:375-393 pubmed publisher
Schultz V, Barrie J, Donald C, Crawford C, Mullin M, Anderson T, et al. Oligodendrocytes are susceptible to Zika virus infection in a mouse model of perinatal exposure: Implications for CNS complications. Glia. 2021;69:2023-2036 pubmed publisher
McGonigal R, Barrie J, Yao D, Black L, McLaughlin M, Willison H. Neuronally expressed a-series gangliosides are sufficient to prevent the lethal age-dependent phenotype in GM3-only expressing mice. J Neurochem. 2021;158:217-232 pubmed publisher
Doroshenko E, Drohomyrecky P, Gower A, Whetstone H, Cahill L, Ganguly M, et al. Peroxisome Proliferator-Activated Receptor-δ Deficiency in Microglia Results in Exacerbated Axonal Injury and Tissue Loss in Experimental Autoimmune Encephalomyelitis. Front Immunol. 2021;12:570425 pubmed publisher
Maggiore J, Burrell J, Browne K, Katiyar K, Laimo F, Ali Z, et al. Tissue engineered axon-based "living scaffolds" promote survival of spinal cord motor neurons following peripheral nerve repair. J Tissue Eng Regen Med. 2020;14:1892-1907 pubmed publisher
Pérez Luz S, Loría F, Katsu Jiménez Y, Oberdoerfer D, Yang O, Lim F, et al. Altered Secretome and ROS Production in Olfactory Mucosa Stem Cells Derived from Friedreich's Ataxia Patients. Int J Mol Sci. 2020;21: pubmed publisher
von Saucken V, Jay T, Landreth G. The effect of amyloid on microglia-neuron interactions before plaque onset occurs independently of TREM2 in a mouse model of Alzheimer's disease. Neurobiol Dis. 2020;145:105072 pubmed publisher
Cignarella F, Filipello F, Bollman B, Cantoni C, Locca A, Mikesell R, et al. TREM2 activation on microglia promotes myelin debris clearance and remyelination in a model of multiple sclerosis. Acta Neuropathol. 2020;140:513-534 pubmed publisher
Cooper M, Pasini S, Lambert W, D Alessandro K, Yao V, Risner M, et al. Redistribution of metabolic resources through astrocyte networks mitigates neurodegenerative stress. Proc Natl Acad Sci U S A. 2020;117:18810-18821 pubmed publisher
Lavoie Cardinal F, Bilodeau A, Lemieux M, Gardner M, Wiesner T, Laramée G, et al. Neuronal activity remodels the F-actin based submembrane lattice in dendrites but not axons of hippocampal neurons. Sci Rep. 2020;10:11960 pubmed publisher
Fleuriet J, Willoughby C, Kueppers R, Mustari M, McLoon L. Eye alignment changes caused by sustained GDNF treatment of an extraocular muscle in infant non-human primates. Sci Rep. 2020;10:11927 pubmed publisher
Mahajan K, Nakamura K, Cohen J, Trapp B, Ontaneda D. Intrinsic and Extrinsic Mechanisms of Thalamic Pathology in Multiple Sclerosis. Ann Neurol. 2020;88:81-92 pubmed publisher
Berger S, Stattmann M, Cicvaric A, Monje F, Coiro P, Hotka M, et al. Severe hydroxymethylbilane synthase deficiency causes depression-like behavior and mitochondrial dysfunction in a mouse model of homozygous dominant acute intermittent porphyria. Acta Neuropathol Commun. 2020;8:38 pubmed publisher
Cortese A, Tozza S, Yau W, Rossi S, Beecroft S, Jaunmuktane Z, et al. Cerebellar ataxia, neuropathy, vestibular areflexia syndrome due to RFC1 repeat expansion. Brain. 2020;143:480-490 pubmed publisher
Ferrer I, Andrés Benito P, Zelaya M, Aguirre M, Carmona M, Ausín K, et al. Familial globular glial tauopathy linked to MAPT mutations: molecular neuropathology and seeding capacity of a prototypical mixed neuronal and glial tauopathy. Acta Neuropathol. 2020;139:735-771 pubmed publisher
Moszczynski A, Harvey M, Fulcher N, de Oliveira C, McCunn P, Donison N, et al. Synergistic toxicity in an in vivo model of neurodegeneration through the co-expression of human TDP-43M337V and tauT175D protein. Acta Neuropathol Commun. 2019;7:170 pubmed publisher
Wang S, Zhang Z, Almenar Queralt A, Leem J, DerMardirossian C, Roth D, et al. Caveolin-1 Phosphorylation Is Essential for Axonal Growth of Human Neurons Derived From iPSCs. Front Cell Neurosci. 2019;13:324 pubmed publisher
Generous A, Harrison O, Troyanovsky R, Mateo M, Navaratnarajah C, Donohue R, et al. Trans-endocytosis elicited by nectins transfers cytoplasmic cargo, including infectious material, between cells. J Cell Sci. 2019;132: pubmed publisher
McCanney G, Lindsay S, McGrath M, Willison H, Moss C, Bavington C, et al. The Use of Myelinating Cultures as a Screen of Glycomolecules for CNS Repair. Biology (Basel). 2019;8: pubmed publisher
Miguel Hidalgo J, Carter K, Deloach P, Sanders L, Pang Y. Glucocorticoid-Induced Reductions of Myelination and Connexin 43 in Mixed Central Nervous System Cell Cultures Are Prevented by Mifepristone. Neuroscience. 2019;: pubmed publisher
Stumpf S, Berghoff S, Trevisiol A, Spieth L, Düking T, Schneider L, et al. Ketogenic diet ameliorates axonal defects and promotes myelination in Pelizaeus-Merzbacher disease. Acta Neuropathol. 2019;138:147-161 pubmed publisher
Gomez Suaga P, Perez Nievas B, Glennon E, Lau D, Paillusson S, Mórotz G, et al. The VAPB-PTPIP51 endoplasmic reticulum-mitochondria tethering proteins are present in neuronal synapses and regulate synaptic activity. Acta Neuropathol Commun. 2019;7:35 pubmed publisher
McWilliams I, Kielczewski J, Ireland D, Sykes J, Lewkowicz A, Konduru K, et al. Pseudovirus rVSVΔG-ZEBOV-GP Infects Neurons in Retina and CNS, Causing Apoptosis and Neurodegeneration in Neonatal Mice. Cell Rep. 2019;26:1718-1726.e4 pubmed publisher
McCanney G, McGrath M, Otto T, Burchmore R, Yates E, Bavington C, et al. Low sulfated heparins target multiple proteins for central nervous system repair. Glia. 2019;67:668-687 pubmed publisher
McGonigal R, Barrie J, Yao D, McLaughlin M, Cunningham M, Rowan E, et al. Glial Sulfatides and Neuronal Complex Gangliosides Are Functionally Interdependent in Maintaining Myelinating Axon Integrity. J Neurosci. 2019;39:63-77 pubmed publisher
Kubo A, Misonou H, Matsuyama M, Nomori A, Wada Kakuda S, Takashima A, et al. Distribution of endogenous normal tau in the mouse brain. J Comp Neurol. 2019;527:985-998 pubmed publisher
Wang X, Li R, Zacharek A, Landschoot Ward J, Wang F, Wu K, et al. Administration of Downstream ApoE Attenuates the Adverse Effect of Brain ABCA1 Deficiency on Stroke. Int J Mol Sci. 2018;19: pubmed publisher
Iglesias J, Crampsie S, Strand C, Tachrount M, Thomas D, Holton J. Effect of Fluorinert on the Histological Properties of Formalin-Fixed Human Brain Tissue. J Neuropathol Exp Neurol. 2018;77:1085-1090 pubmed publisher
Heuss N, Pierson M, Roehrich H, McPherson S, Gram A, Li L, et al. Optic nerve as a source of activated retinal microglia post-injury. Acta Neuropathol Commun. 2018;6:66 pubmed publisher
Villalon E, Barry D, Byers N, Frizzi K, Jones M, Landayan D, et al. Internode length is reduced during myelination and remyelination by neurofilament medium phosphorylation in motor axons. Exp Neurol. 2018;306:158-168 pubmed publisher
Yu G, Zheng S, Zhang H. Inhibition of myeloperoxidase by N-acetyl lysyltyrosylcysteine amide reduces experimental autoimmune encephalomyelitis-induced injury and promotes oligodendrocyte regeneration and neurogenesis in a murine model of progressive multiple sclerosis. Neuroreport. 2018;29:208-213 pubmed publisher
Andrew R, Fernandez C, Stanley M, Jiang H, Nguyen P, Rice R, et al. Lack of BACE1 S-palmitoylation reduces amyloid burden and mitigates memory deficits in transgenic mouse models of Alzheimer's disease. Proc Natl Acad Sci U S A. 2017;114:E9665-E9674 pubmed publisher
Khedraki A, Reed E, Romer S, Wang Q, Romine W, Rich M, et al. Depressed Synaptic Transmission and Reduced Vesicle Release Sites in Huntington's Disease Neuromuscular Junctions. J Neurosci. 2017;37:8077-8091 pubmed publisher
Cumberworth S, Barrie J, Cunningham M, de Figueiredo D, Schultz V, Wilder Smith A, et al. Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected. Acta Neuropathol Commun. 2017;5:50 pubmed publisher
Mu L, Sobotka S, Chen J, Nyirenda T. Reinnervation of denervated muscle by implantation of nerve-muscle-endplate band graft to the native motor zone of the target muscle. Brain Behav. 2017;7:e00668 pubmed publisher
van Nierop G, van Luijn M, Michels S, Melief M, Janssen M, Langerak A, et al. Phenotypic and functional characterization of T cells in white matter lesions of multiple sclerosis patients. Acta Neuropathol. 2017;134:383-401 pubmed publisher
Llorens F, Thüne K, Sikorska B, Schmitz M, Tahir W, Fernández Borges N, et al. Altered Ca2+ homeostasis induces Calpain-Cathepsin axis activation in sporadic Creutzfeldt-Jakob disease. Acta Neuropathol Commun. 2017;5:35 pubmed publisher
Sama R, Fallini C, Gatto R, McKeon J, Song Y, Rotunno M, et al. ALS-linked FUS exerts a gain of toxic function involving aberrant p38 MAPK activation. Sci Rep. 2017;7:115 pubmed publisher
Ling H, Morris H, Neal J, Lees A, Hardy J, Holton J, et al. Mixed pathologies including chronic traumatic encephalopathy account for dementia in retired association football (soccer) players. Acta Neuropathol. 2017;133:337-352 pubmed publisher
Berghoff S, Gerndt N, Winchenbach J, Stumpf S, Hosang L, Odoardi F, et al. Dietary cholesterol promotes repair of demyelinated lesions in the adult brain. Nat Commun. 2017;8:14241 pubmed publisher
Cui C, Ye X, Chopp M, Venkat P, Zacharek A, Yan T, et al. miR-145 Regulates Diabetes-Bone Marrow Stromal Cell-Induced Neurorestorative Effects in Diabetes Stroke Rats. Stem Cells Transl Med. 2016;5:1656-1667 pubmed
Li T, Braunstein K, Zhang J, Lau A, Sibener L, Deeble C, et al. The neuritic plaque facilitates pathological conversion of tau in an Alzheimer's disease mouse model. Nat Commun. 2016;7:12082 pubmed publisher
Riku Y, Watanabe H, Yoshida M, Mimuro M, Iwasaki Y, Masuda M, et al. Marked Involvement of the Striatal Efferent System in TAR DNA-Binding Protein 43?kDa-Related Frontotemporal Lobar Degeneration and Amyotrophic Lateral Sclerosis. J Neuropathol Exp Neurol. 2016;: pubmed
Stark D, Coffey N, Pancoast H, Arnold L, Walker J, Vallée J, et al. Ephrin-A3 promotes and maintains slow muscle fiber identity during postnatal development and reinnervation. J Cell Biol. 2015;211:1077-91 pubmed publisher
Cui X, Chopp M, Zacharek A, Cui C, Yan T, Ning R, et al. D-4F Decreases White Matter Damage After Stroke in Mice. Stroke. 2016;47:214-20 pubmed publisher
Takahashi M, Ikemura M, Oka T, Uchihara T, Wakabayashi K, Kakita A, et al. Quantitative correlation between cardiac MIBG uptake and remaining axons in the cardiac sympathetic nerve in Lewy body disease. J Neurol Neurosurg Psychiatry. 2015;86:939-44 pubmed publisher
Redondo J, Kemp K, Hares K, Rice C, Scolding N, Wilkins A. Purkinje Cell Pathology and Loss in Multiple Sclerosis Cerebellum. Brain Pathol. 2015;25:692-700 pubmed publisher
McLean N, Popescu B, Gordon T, Zochodne D, Verge V. Delayed nerve stimulation promotes axon-protective neurofilament phosphorylation, accelerates immune cell clearance and enhances remyelination in vivo in focally demyelinated nerves. PLoS ONE. 2014;9:e110174 pubmed publisher
Maire C, Ramkissoon S, Hayashi M, Haidar S, Ramkissoon L, diTomaso E, et al. Pten loss in Olig2 expressing neural progenitor cells and oligodendrocytes leads to interneuron dysplasia and leukodystrophy. Stem Cells. 2014;32:313-26 pubmed publisher
Barry D, Stevenson W, Bober B, Wiese P, Dale J, Barry G, et al. Expansion of neurofilament medium C terminus increases axonal diameter independent of increases in conduction velocity or myelin thickness. J Neurosci. 2012;32:6209-19 pubmed publisher
Kiryu Seo S, Ohno N, Kidd G, Komuro H, Trapp B. Demyelination increases axonal stationary mitochondrial size and the speed of axonal mitochondrial transport. J Neurosci. 2010;30:6658-66 pubmed publisher
Choi Y, Di Nardo A, Kramvis I, Meikle L, Kwiatkowski D, Sahin M, et al. Tuberous sclerosis complex proteins control axon formation. Genes Dev. 2008;22:2485-95 pubmed publisher
Maurel P, Einheber S, Galinska J, Thaker P, Lam I, Rubin M, et al. Nectin-like proteins mediate axon Schwann cell interactions along the internode and are essential for myelination. J Cell Biol. 2007;178:861-74 pubmed
Edgar J, McLaughlin M, Yool D, Zhang S, Fowler J, Montague P, et al. Oligodendroglial modulation of fast axonal transport in a mouse model of hereditary spastic paraplegia. J Cell Biol. 2004;166:121-31 pubmed
Rao M, Campbell J, Yuan A, Kumar A, Gotow T, Uchiyama Y, et al. The neurofilament middle molecular mass subunit carboxyl-terminal tail domains is essential for the radial growth and cytoskeletal architecture of axons but not for regulating neurofilament transport rate. J Cell Biol. 2003;163:1021-31 pubmed
Xia C, Roberts E, Her L, Liu X, Williams D, Cleveland D, et al. Abnormal neurofilament transport caused by targeted disruption of neuronal kinesin heavy chain KIF5A. J Cell Biol. 2003;161:55-66 pubmed
Franzen R, Tanner S, Dashiell S, Rottkamp C, Hammer J, Quarles R. Microtubule-associated protein 1B: a neuronal binding partner for myelin-associated glycoprotein. J Cell Biol. 2001;155:893-8 pubmed
Sakurai T, Lustig M, Babiarz J, Furley A, Tait S, Brophy P, et al. Overlapping functions of the cell adhesion molecules Nr-CAM and L1 in cerebellar granule cell development. J Cell Biol. 2001;154:1259-73 pubmed
Tomishima M, Enquist L. A conserved alpha-herpesvirus protein necessary for axonal localization of viral membrane proteins. J Cell Biol. 2001;154:741-52 pubmed
Yin X, Kidd G, Wrabetz L, Feltri M, Messing A, Trapp B. Schwann cell myelination requires timely and precise targeting of P(0) protein. J Cell Biol. 2000;148:1009-20 pubmed
product information
Antigen :
Neurofilament H
Apps. Abbrev. :
IHC-P, WB, ICC
Cat # :
801602
Clone :
SMI 31
Item :
Purified anti-Neurofilament H (NF-H), Phosphorylated
Isotype :
Mouse IgG1, κ
Other Names :
Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
Size :
25 μL
Price (USD) :
112 USD
Reactivity :
Human, Mouse, Rat
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Application Notes :
Additional reported applications (for the relevant formats) include: Western blotting^1, immunohistochemistry^2,4, and immunocytochemistry^4.SMI 31 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, which chicken and frog (Xenopus). Immunocytochemically, SMI 31 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 31. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 31 may be found also in neuronal cell bodies. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures with SMI 31 by agents that induce stress-activated protein kinase. In its reaction with paired helical filaments in hereditary inclusion body myopathy, SMI 31 colocalizes with nitric oxide synthase, suggesting that oxidative stress may play a role in the pathogenic cascade of such degenerative diseases. SMI 31 co-immunoprecipitates neurofilament-associated kinase (NAK 115) via reaction of the antibody with the tail domain of neurofilament H.;
more info or order :
company information
BioLegend
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San Diego, CA 92121
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https://www.biolegend.com
headquarters: USA