Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Human CES1 (carrier-free)

794606

100 μg

1026 USD

BA

794606

Recombinant Human CES1 (carrier-free)

Liver Carboxylesterase 1, Serine Esterase 1 (SES1), Human Monocyte Serine Esterase (HMSE), Cholesterol Ester Hydrolase (CESH)

100 μg

1026 USD

Human

ADD EXPRESSION SYSTEM HERE

RECOM

Human CES1 Enzymatic AssayHuman carboxylesterase 1 hydrolyzes the carboxylic acid substrate, 4-nitrophenyl acetate. The increase of hydrolyzed product is monitored by an increase in absorbance at 400 nm. The specific activity is > 6,000 pmol/min/µg in the presence of 0.04 µg of recombinant human CES1.Materials;Assay buffer: 50 mM Tris, pH 7.5;Recombinant human CES1;Substrate: 4-nitrophenyl acetate (4-NPA) (Sigma, Cat. No. N-8130), 100 mM stock in acetone;96-well clear plate (Costar, Cat. No. 92592);Plate reader (Model: SpectraMax Plus by Molecular Devices or equivalent);