Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Human SUMO3-GFP Protease Substrate (carrier-free)

793306

100 μg

211 USD

BA

793306

Recombinant Human SUMO3-GFP Protease Substrate (carrier-free)

Small Ubiquitin Like Modifier 3, Small Ubiquitin-Related Modifier 3, SMT3H1, SUMO-3, SMT3A

100 μg

211 USD

Human

ADD EXPRESSION SYSTEM HERE

RECOM

Human SUMO3-GFP Protocol:;Human SUMO3-GFP is digested by different proteases.;Incubate mixtures of 5 µg of SUMO3-GFP with protease (eigther SENP2, thrombin, enterokinase (ENTK), 3C protease, factor Xa, TEV) for 16 hours at 25°C.;Run SDS-PAGE gel with mixtures.;Assay Buffer for Each Protease Mixture:;a) Human SENP2 and 3C protease buffer: 20 mM Tris, pH8, 100 mM NaCl;b) Thrombin and factor Xa buffer: 20 mM Tris, pH 8, 100 mM NaCl, 2 mM CaCl2;c) Enterokinese buffer: 20 mM Tris, pH 8, 100 mM NaCl;d) TEV buffer: 20 mM Tris, pH 8, 100 mM NaCl, 1 mM DTT, 0.5 mM EDTA