Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Human ACE (carrier-free)

790606

100 μg

335 USD

BA

790606

Recombinant Human ACE (carrier-free)

DPC, ACE1, DPC1, CD143, CD143 Antigen, Angiotensin-Converting Enzyme, Angiotensin I Converting Enzyme, Dipeptidyl Carboxypeptidase 1

100 μg

335 USD

Human

ADD EXPRESSION SYSTEM HERE

RECOM

Human ACE Enzymatic AssayHuman ACE activity is measured by its ability to cleave the fluorogenic peptide substrate MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(DNP)-OH. The increase of the product is monitored by increase in intensity of fluorescence at 405 nm with excitation at 320 nm. Perform the assay rapidly. The specific activity is > 1,000 pmol/min/ug in the presence of 0.020 µg of recombinant human ACE.Materials;Assay buffer: 50 mM MES, pH 6.5 (at room temperature);Human ACE/CD143;Fluorogenic peptide substrate: MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(DNP)-OH;F16 Black Maxisorp Plate (Nunc, Cat. No. 475515);Activity Assay Procedures;Dilute the substrate in assay buffer at 20 µM. Wrap substrate container in aluminium foil.;In assay buffer, prepare 50 µL of serial dilutions of human ACE. Start at a concentration of 3.2 µg/mL and dilute 1 to 2 six times after.;Add 50 µL of 20 µM substrate to the wells with 50 µL of prepared dilutions.;Include a substrate black with 50 µL of assay buffer and 50 µL of 20 µM substrate without any human ACE.;Read at excitation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes.;Calculate specific activity using the following formula:;Specific Activity (pmol/min/µg)= ((Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)) amount of enzyme (µg);Final assay conditions per well:Human ACE: 0.16, 0.08, 0.04, 0.02, 0.01, 0.005, 0.0025 µgSubstrate: 10 µMBioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;