Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Human ACPP (carrier-free)

789302

10 μg

89 USD

BA

789302

Recombinant Human ACPP (carrier-free)

Prostatic acid phosphatase, PAP, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, Thiamine monophsophatase, TMPase, ACPP

10 μg

89 USD

Human

ADD EXPRESSION SYSTEM HERE

RECOM

Human ACPP Activity Assay;Dilute the recombinant human ACPP at 0.1 µg/mL in assay buffer.;Dilute the substrate at 2 mM in assay buffer.;In a plate, combine 50 µL of hACPP and 50 µL of 2 mM Substrate. Include a substrate blank containing 50 µL of assay buffer and 50 µL of 2 mM substrate.;Incubate the reaction at room temperature for 5 minutes.;Add 100 µL of 0.2 M NaOH to stop the reaction and develop the color.;Read the absorbance in endpoint mode at 410 nm.;Calculate the specific activity:;Specific Activity (pmol/min/µg)= ((Adjusted Vmax* (OD/min) x Conversion Factor** (pmol/OD)) Incubation time (min) x amount of enzyme (µg);*Adjusted for substrate blank**Derived using calibration standard p-Nitrophenol (Sigma-Aldrich, Catalog No. 241326).Per Well:• Recombinant human ACPP: 0.005 µg• Substrate: 0.5 mMMaterials• Assay buffer: 50 mM NaOAc, pH 4.5• Recombinant human Prostatic Acid Phosphatase/ACPP (hACPP)• Substrate: p-Nitrophenyl phosphate (Sigma-Aldrich, Catalog No. N2765)• 96-well clear plate (Costar, Catalog No. 92592)• Plate reader (Model: SpectraMax Plus by Molecular Devices) or equivalent)• NaOH, 0.2 M in deionized water; BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;