Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Mouse ACP5 (carrier-free)

789204

25 μg

347 USD

BA

789204

Recombinant Mouse ACP5 (carrier-free)

TRAP, TRACP, TRACP5a, TRACP5b, Tartrate-Resistant Acid ATPase, Acid Phosphatase 5 Tartrate Resistant, Type 5 Acid Phosphatase

25 μg

347 USD

Mouse

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RECOM

ALP5 Assay Procedure;In assay buffer, prepare dilution of mouse ACP5 at 0.1 µg/mL;Add 50 µL of mACP5 and 50 µL of prepared 2 mM substrate to plate. Include a blank for the substrate containing 50 µL assay buffer and 50 µL of 2 mM substrate.;At room temperature, incubate plate for 10 minutes in the dark.;To develop the color and stop the reaction, add 100 µL of 0.2 M NaOH.;Read (top read) absorbance in endpoint mode at 410 nm.;Calculate the specific activity:;Specific Activity (pmol/min/µg) = Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)Incubation time (min) x amount of enzyme (µg)*Adjusted for the substrate blank**Derived using calibration standard p-Nitrophenol (Sigma-Aldrich, Catalog # 241326).Per Well:;Recombinant mACP5: 0.005 µg;Substrate: 0.5 mM;Reagents:;Assay Buffer: 50 mM NaOAc, pH 5.0;Mouse TRACP/PAP/ACP5;Substrate: p-Nitrophenyl phosphate (Sigma-Aldrich, Catalog # N2765);96-well Clear Plate (Costar, Catalog # 92592);Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent;NaOH, 0.2 M in deionized water;BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.