Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Mouse ALPL (carrier-free)

787804

25 μg

370 USD

BA

787804

Recombinant Mouse ALPL (carrier-free)

ALP, Akp2, TNAP, Akp-2, APTNAP, TNSALP

25 μg

370 USD

Mouse

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RECOM

Mouse ALPL Activity Assay;In assay buffer, prepare serial dilutions of mALPL in 1 to 2 ratios. Starting concentration is 40 ng/mL.;In assay buffer, prepare the substrate at 50 µM.;Add 50 µL of prepared mALPL dilutions to the assay plate.;Begin the reaction by adding 50 µL of substrate to the plate.;Take readings of the plate at excitation, 365nm, and emission, 445nm, wavelengths in kinetic mode for 5 minutes.;Using the following equation, calculate the specific activity.;Specific Activity (pmol/min/µg) = [Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)]/ amount of enzyme (µg)Per Well:;mALPL amount: 2, 1, 0.5, 0.25, 0.125, 0.0625, 0.03125 ng;Substrate: 25 µM;Materials:;Assay Buffer: 50 mM Tris, 1 mM MgCl2, pH 9.0;Recombinant Mouse Alkaline Phosphatase/ALPL (mALPL);Substrate: 4-Methylumbelliferlyphosphate (4-MUP);F16 Black Maxisorp Plate;Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent.;BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;