Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Mouse FAP (carrier-free)

787302

10 μg

159 USD

BA

787302

Recombinant Mouse FAP (carrier-free)

Fibroblast Activation Protein Alpha, FAPalpha, Serine Integral Membrane Protease, DPPIV, Separase, Prolyl Endopeptidase FAP, Dipeptidyl Peptidase FAP, Surface-Expressed Protease, Post-Proline Cleaving Enzyme Fibroblast activation protein

10 μg

159 USD

Mouse

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RECOM

Mouse FAP Activity assayMouse FAP activity is measured by its ability to cleave a fluorogenic peptide substrate Z-Gly-Pro-AMC. The increase of the product formation is monitored by an increase in intensity of fluorescence at 460 nm with excitation at 380 nm.Materials;Assay Buffer: pH 7.5, 50 mM Tris, 1.0 M NaCl, 0.1% BSA (w/v);Recombinant Mouse FAP;FAP substrate: Z-Gly-Pro-AMC;Activity assay procedures;Dilute mFAP in the assay buffer at 0.2 µg/mL.;Dilute the substrate at 100 µM in Assay Buffer.;Load into a well plate 50 µL of the 0.2 ng/µL mFAP and start the reaction by adding 50 µL of 100 µM Substrate. Include a substrate blank containing 50 µL of Assay Buffer and 50 µL of 100 µM Substrate solution.;Read the product formation by measuring 380/460 nm (Excitation/Emission) in kinetic mode for 5 minutes.;The final mFAP concentration is 0.01 µg/100 µL reaction.;The substrate is 50 µM.;BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;