Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Mouse CD39 (carrier-free)

775502

10 μg

206 USD

BA

775502

Recombinant Mouse CD39 (carrier-free)

Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPDase1), CD39 antigen, ecto-ATP diphosphohydrolase 1, ecto-ATPase 1, ectoapyrase, lymphoid cell activation antigen

10 μg

206 USD

Mouse

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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com. Mouse CD39 Activity assay; Solutions:;Enzyme: Recombinant mouse CD39;Substrate: Adenosine triphosphate (ATP), 10 uM stock solution in deionized water.;Assay buffer: 25 mM Tris, 5 mM CaCl2, pH 7.5;Malachite Green Phosphate Assay Kit;Procedure;Phosphate Standard curve. From a 1 M Phosphate Standard solution (included in the Malachite Green Phosphate Assay Kit) prepare 100 µM solution of the standard in the assay buffer (phosphate stock). Set six points of 1:2 serial dilutions for the standard curve from 2.5 to 0.39 nmol/well.;Prepare (1:2) serial dilutions of recombinant mouse CD39 in Assay Buffer. The top concentration will be 0.5 ng/well in 25 µl.;Prepare the substrate (ATP) solution at 100 µM and transfer 25 µl to each well containing the recombinant mouse CD39 and the blanks (buffer only).;Incubate the plate at 37°C for 30 minutes.;Add 5 ul of acidic solution (included in the Malachite Green Phosphate Assay Kit) to all the wells, mix gently and incubate for 10 minutes at room temperature.;Add 15 µl of Malachite Green Blue solution to each well. Mix gently and incubate 20 minutes at Room temperature.;Measure the absorbance using a microplate reader set at 620 nm.;