Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Human ENPP6 (carrier-free)

773204

25 μg

229 USD

BA

773204

Recombinant Human ENPP6 (carrier-free)

Ectonucleotide Pyrophosphatase, Phosphodiesterase Family Member 6, NPP6, GPC-Cpde, Ectonucleotide Pyrophosphatase, Phosphodiesterase 6

25 μg

229 USD

Human

ADD EXPRESSION SYSTEM HERE

RECOM

Bioassay Procedure and Materials;Assay Buffer: 50 mM Tris, 0.5 M NaCl, pH 9.0;Recombinant human ENPP6; Substrate: O-(4-Nitrophenylphosphoryl) choline (Sigma, Catalog # N5879), 500 mM stock in deionized water;96-well clear plate;Plate reader (model: SpectraMax Plus by Molecular Devices) or equivalent;Dilute the recombinant ENPP6 to 0.4 µg/mL in assay buffer.;Dilute the substrate at room temperature to 2 mM in assay buffer.;Load 50 µL of 0.4 ng/µL of recombinant ENPP6 in a clear strip well plate.;Start the reaction by adding 50 µL of 2 mM substrate. Include a substrate blank containing 50 µL assay buffer and 50 µL of substrate.;Read at 405 nm (absorbance) in kinetic mode for 5 minutes.;Calculate specific activity:; Specific Activity (pmol/min/µg) =;Adjusted Vmax* (OD/min) x Conversion Factor** (pmol/OD);;amount of enzyme (µg);; *Adjusted for Substrate Blank **Derived using calibration standard p-Nitrophenol (Sigma, Catalog # 241326).;The final concentration of human recombinant ENPP6 is 0.2mg/ml (0.02 mg);The final concentration of substrate is 1mM BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;