Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Human FAP (carrier-free)

768904

25 μg

335 USD

BA

768904

Recombinant Human FAP (carrier-free)

FAP, FAP-alpha, Fibroblast Activation Protein alpha, fibroblast activation protein, alpha, Integral membrane serine protease, Seprase, DPP4

25 μg

335 USD

Human

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RECOM

Human FAP Activity assay; Human FAP activity is measured by its ability to cleave a fluorogenic peptide substrate Z-Gly-Pro-AMC. The increase of the product formation is monitored by increase in intensity of fluorescence at 460 nm with excitation at 380 nm.; Materials;Assay Buffer: pH 7.5, 50 mM Tris, 1.0 M NaCl, 0.1% BSA (w/v);Recombinant Human FAP;FAP substrate: Z-Gly-Pro-AMC; Activity assay procedures;Dilute hFAP in the assay buffer at 0.2 µg/mL.;Dilute the substrate at 100 µM in Assay Buffer.;Load into a well plate 50 µL of the 0.2 ng/µL hFAP and start the reaction by adding 50 µL of 100 µM Substrate. Include a substrate blank containing 50 µL of Assay Buffer and 50 µL of 100 µM Substrate solution.;Read the product formation by measuring 380/460 nm (Excitation/Emission) in kinetic mode for 5 minutes.; The final hFAP concentration is 0.01 µg/100 µL reaction.;The substrate is 50 µM.;BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;