Recombinant Human ACHE (carrier-free) | BioLegend 768106 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

protein

product name :

Recombinant Human ACHE (carrier-free)

catalog :

768106

quantity :

100 μg

price :

850 USD

citations: 3

Reference
Sinclair L, Barthelemy C, Cantrell D. Single Cell Glucose Uptake Assays: A Cautionary Tale. Immunometabolism. 2020;2:e200029 pubmed publisher
Chew H, De Lima P, Gonzalez Cruz J, Banushi B, Echejoh G, Hu L, et al. Endocytosis Inhibition in Humans to Improve Responses to ADCC-Mediating Antibodies. Cell. 2020;180:895-914.e27 pubmed publisher
Daglas M, Draxler D, Ho H, McCutcheon F, Galle A, Au A, et al. Activated CD8+ T Cells Cause Long-Term Neurological Impairment after Traumatic Brain Injury in Mice. Cell Rep. 2019;29:1178-1191.e6 pubmed publisher

product information

Antigen :

ACHE

Apps. Abbrev. :

Cat # :

768106

Item :

Recombinant Human ACHE (carrier-free)

Other Names :

Macrophage metalloelastase (MME), Matrix metalloproteinase 12

Size :

100 μg

Price (USD) :

850 USD

Reactivity :

Human

Host :

CHO cells

Conjugate/Tag/Label :

RECOM

Application Notes :

Human ACHE Activity Assay . Human ACHE activity is measured by its ability to cleave the acetylthiocholine. The accumulation of the product cleavage is monitored by increase in intensity of absorbance at 405 nm in the presence of DTNB. . Materials and Buffers Human ACHE (Predicted MW: 60.7 kDa) Assay Buffer: 0.1 M sodium phosphate, 0.05% Brij-35, pH 7.5. 5 ,5 -Dithiobis(2-nitrobenzoic acid) (DTNB) ACHE Substrate: Acetylthiocholine (ATC) . Assay Procedures Dilute rhACHE to 0.002 ug/mL. Dilute substrate to 200 uM in assay buffer containing 200 uM DTNB. Load 50 uL of rhACHE solution to clear plate. Include a control containing assay buffer only. Start reaction by adding 50 uL substrate/DTNB mixture. Read plate in kinetic mode for 5 minutes by monitoring absorbance at 405 nm. Calculate specific activity. Conversion factor of the product 5-thio-2-nitrobenzoic acid by using extinction coefficient 13260 M -1 cm -1 , 0.32 cm of light path correction, and total volume of 100 uL: 23.6 pmol/mAu rhACHE: 0.0001 ug DTNB: 100 uM ATC: 100 uM . BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee . If you have any concerns, contact us at tech@biolegend.com .