Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Human ACHE (carrier-free)

768104

25 μg

370 USD

BA

768104

Recombinant Human ACHE (carrier-free)

Macrophage metalloelastase (MME), Matrix metalloproteinase 12

25 μg

370 USD

Human

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RECOM

Human ACHE Activity Assay;Human ACHE activity is measured by its ability to cleave the acetylthiocholine. The accumulation of the product cleavage is monitored by increase in intensity of absorbance at 405 nm in the presence of DTNB.;Materials and Buffers;Human ACHE (Predicted MW: 60.7 kDa);Assay Buffer: 0.1 M sodium phosphate, 0.05% Brij-35, pH 7.5.;5',5'-Dithiobis(2-nitrobenzoic acid) (DTNB);ACHE Substrate: Acetylthiocholine (ATC);Assay Procedures;Dilute rhACHE to 0.002 µg/mL.;Dilute substrate to 200 µM in assay buffer containing 200 µM DTNB.;Load 50 µL of rhACHE solution to clear plate. Include a control containing assay buffer only.;Start reaction by adding 50 µL substrate/DTNB mixture.;Read plate in kinetic mode for 5 minutes by monitoring absorbance at 405 nm.;Calculate specific activity. Conversion factor of the product 5-thio-2-nitrobenzoic acid by using extinction coefficient 13260 M^-1cm^-1, 0.32 cm of light path correction, and total volume of 100 µL: 23.6 pmol/mAu;rhACHE: 0.0001 µg;DTNB: 100 µM;ATC: 100 µM;BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;