Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Mouse BACE1 (Beta-Secretase 1) (carrier-free)

765802

10 μg

112 USD

BA

765802

Recombinant Mouse BACE1 (Beta-Secretase 1) (carrier-free)

Beta-Secretase-1, Beta-site APP-cleaving enzyme 1, aspartyl protease 2 (ASP-2), Memapsin 2, Secretase, BACE

10 μg

112 USD

Mouse

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RECOM

Mouse BACE1 Activity assay; Mouse BACE1 activity is measured by its ability to cleave a fluorogenic peptide substrate Mca-SEVNLDAEFRK(Dnp)RR-NH2. The increase of the product is monitored by increase in intensity of emission at 405 nm with excitation at 320 nm. This enzyme is in active form and does not require further activation.; Materials;Assay Buffer: pH 4.0, 50 mM Citrate, 0.1% BSA;Recombinant Human BACE1;BACE1 Substrate: Mca-SEVNLDAEFRK(Dnp)RR-NH2 ; Activity assay procedures;Dilute mBACE1 in the assay buffer at 10 µg/mL (10 ng/µL).;Dilute the substrate at 10 µM in Assay Buffer.;Load into a well plate 50 μL of the 10 ng/μL mBACE1 and start the reaction by adding 50 μL of 20 μM Substrate. Include a substrate blank containing 50 μL of Assay Buffer and 50 μL of 20 μM Substrate solution.;Read the product formation by measuring 320/405 nm (Excitation/Emission) in kinetic mode for 5 minutes.; The final mBACE1 concentration is 5 µg/mL (0.5 µg) and the substrate is 10 µM.;BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;