Recombinant Human MMP-12 (carrier-free) | BioLegend 764206 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

protein

product name :

Recombinant Human MMP-12 (carrier-free)

catalog :

764206

quantity :

100 μg

price :

950 USD

citations: 1

Reference
Gruber C, Patel R, Trachtman R, Lepow L, Amanat F, Krammer F, et al. Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C). Cell. 2020;183:982-995.e14 pubmed publisher

product information

Antigen :

MMP-12

Apps. Abbrev. :

Cat # :

764206

Item :

Recombinant Human MMP-12 (carrier-free)

Other Names :

Macrophage metalloelastase (MME), Matrix metalloproteinase 12

Size :

100 μg

Price (USD) :

950 USD

Reactivity :

Human

Host :

CHO cells

Conjugate/Tag/Label :

RECOM

Application Notes :

Human MMP-12 Activity assay . . Human MMP-12 (hMMP-12) activity is measured by its ability to cleave a fluorogenic peptide substrate Mca-PLGL(Dpa)AR after its activation in the presence of p-Aminophenylmercuric acid. The increase of the product is monitored by increase in intensity of fluorescence at 405 nm with excitation at 320 nm. This protein is in the latent form and needs to be activated for bioassay. . . Materials Assay Buffer: TCNB (50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35, pH 7.5) Recombinant Human MMP-12 MCa-PLGL(Dpa)AR (AnaSpec Cat # AS27076; 2 mM in DMSO) P-Aminophenylmercuric acid (Calbiochem Cat# 164610; 100 mM in DMSO) . . Activity assay procedures Dilute hMMP-12 in the assay buffer at 100 ug/mL. Activate hMMP-12 by adding APMA to a final concentration of 1 mM. Incubate the activating mixture for 1 hr at 37 C. Dilute the activated hMMP-12 to 0.4 ug/mL (0.4 ng/uL) in Assay Buffer. Dilute the substrate at 20 uM in Assay Buffer. Load into a well plate 50 uL of the 0.4 ng/uL hMM-12 and start the reaction by adding 50 uL of 20 uM Substrate. Include a substrate blank containing 50 uL Assay Buffer and 50 uL of 20 uM Substrate without any hMMP-12. Read the product formation by measuring 320/ 405 nm (Excitation/Emission) in kinetic mode for 5 minutes. . The final hMMP-12 concentration is 0.2 ug/mL (0.2 ng/ul, 20 ng) . The final concentration of the substrate is 10 uM. . BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee . If you have any concerns, contact us at tech@biolegend.com .