Recombinant Human DPP4 (carrier-free) | BioLegend 764108 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

protein

product name :

Recombinant Human DPP4 (carrier-free)

catalog :

764108

quantity :

500 μg

price :

3000 USD

citations: 2

Reference
Coronel M, MARTIN K, Hunckler M, Barber G, O Neill E, Medina J, et al. Immunotherapy via PD-L1-presenting biomaterials leads to long-term islet graft survival. Sci Adv. 2020;6:eaba5573 pubmed publisher
Sledzińska A, Vila de Mucha M, Bergerhoff K, Hotblack A, Demane D, Ghorani E, et al. Regulatory T Cells Restrain Interleukin-2- and Blimp-1-Dependent Acquisition of Cytotoxic Function by CD4+ T Cells. Immunity. 2020;52:151-166.e6 pubmed publisher

product information

Antigen :

DPP4

Apps. Abbrev. :

Cat # :

764108

Item :

Recombinant Human DPP4 (carrier-free)

Other Names :

CD26, Adenosine deaminase binding protein (ADABP), Adenosine deaminase complexing protein2 (ADCP-2), Dipeptidyl peptidase IV (DPP IV)

Size :

500 μg

Price (USD) :

3000 USD

Reactivity :

Human

Host :

CHO cells

Conjugate/Tag/Label :

RECOM

Application Notes :

Human DPP4 Activity assay . . Human DPP4 activity is measured by its ability to cleave a fluorogenic peptide substrate Gly-Pro-AMC. The increase of the product is monitored by increase in intensity of fluorescence at 460 nm with excitation at 380 nm. This protein is in the activated form and does not require further activation for bioassay. The purified recombinant DPP4 behaves as a non-disulfide-bonded dimer based on the analysis of SDS PAGE and gel filtration. . . Materials Assay Buffer: pH 8.0, 25 mM Tris Recombinant Human DPP-4 DPP-4 substrate: Gly-Pro-AMC . . Activity assay procedures Dilute hDPP4 in the assay buffer at 0.1 ug/mL (0.1 ng/uL). Dilute the substrate at 100 uM in Assay Buffer. Load into a well plate 50 uL of the 0.1 ng/uL hDPP4 and start the reaction by adding 50 uL of 100 uM Substrate. Include a substrate blank containing 50 uL of Assay Buffer and 50 uL of 100 uM Substrate solution. Read the product formation by measuring 380/460 nm (Excitation/Emission) in kinetic mode for 5 minutes. . The final hDPP4 concentration is 0.05 ug/mL (0.05 ng/uL; 5 ng) . The final concentration of the substrate is 50 uM. . BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee . If you have any concerns, contact us at tech@biolegend.com .