Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Human DPP4 (carrier-free)

764102

10 μg

206 USD

BA

764102

Recombinant Human DPP4 (carrier-free)

CD26, Adenosine deaminase binding protein (ADABP), Adenosine deaminase complexing protein2 (ADCP-2), Dipeptidyl peptidase IV (DPP IV)

10 μg

206 USD

Human

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RECOM

Human DPP4 Activity assay; Human DPP4 activity is measured by its ability to cleave a fluorogenic peptide substrate Gly-Pro-AMC. The increase of the product is monitored by increase in intensity of fluorescence at 460 nm with excitation at 380 nm. This protein is in the activated form and does not require further activation for bioassay. The purified recombinant DPP4 behaves as a non-disulfide-bonded dimer based on the analysis of SDS PAGE and gel filtration.; Materials;Assay Buffer: pH 8.0, 25 mM Tris;Recombinant Human DPP-4;DPP-4 substrate: Gly-Pro-AMC; Activity assay procedures;Dilute hDPP4 in the assay buffer at 0.1 µg/mL (0.1 ng/µL).;Dilute the substrate at 100 µM in Assay Buffer.;Load into a well plate 50 μL of the 0.1 ng/μL hDPP4 and start the reaction by adding 50 μL of 100 μM Substrate. Include a substrate blank containing 50 μL of Assay Buffer and 50 μL of 100 μM Substrate solution.;Read the product formation by measuring 380/460 nm (Excitation/Emission) in kinetic mode for 5 minutes.;The final hDPP4 concentration is 0.05 µg/mL (0.05 ng/uL; 5 ng);The final concentration of the substrate is 50 µM.;BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;