Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Mouse Cathepsin B (CTSB) (carrier-free)

763004

25 μg

440 USD

BA

763004

Recombinant Mouse Cathepsin B (CTSB) (carrier-free)

CTSB, CPSB, APP secretase, APPS, Cathepsin B1, CB

25 μg

440 USD

Mouse

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RECOM

Cathepsin B Activity Protocol: ;1. Dilute the recombinant mouse Cathepsin B to 10 μg/ml in activation buffer (25 mM MES, 1 mM DTT, pH 5.0) and incubate the protein at 25°C for 3 min. Over activation may result in proteolysis and low activity.2. Dilute the recombinant mouse Cathepsin B to 0.05 μg/ml in assay buffer (25 mM MES, pH 5.0).3. Dilute the fluorogenic peptide substrate (Z-LR-AMC) to 40 μM using assay buffer.4. Perform the assay in a 96-well, black, flat-bottomed plate. Add 50 μl of the diluted, activated Cathepsin B (0.0025 µg/well) to a black well and start the reaction by adding 50 μl of 40 μM substrate. The assay is read on a fluorometer at excitation and emission wavelengths of 380 nm and 460 nm for five minutes (top read). This protein is in the latent form and needs to be activated for bioassay.; BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;