Recombinant Human PLA2G7 (carrier-free) | BioLegend 762904 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

protein

product name :

Recombinant Human PLA2G7 (carrier-free)

catalog :

762904

quantity :

25 μg

price :

375 USD

citations: 1

Reference
Sekine T, Perez Potti A, Rivera Ballesteros O, Stralin K, Gorin J, Olsson A, et al. Robust T Cell Immunity in Convalescent Individuals with Asymptomatic or Mild COVID-19. Cell. 2020;183:158-168.e14 pubmed publisher

product information

Antigen :

PLA2G7

Apps. Abbrev. :

Cat # :

762904

Item :

Recombinant Human PLA2G7 (carrier-free)

Other Names :

Lipoprotein binding phospholipase A2 (Lp-PLA2), LDL-PLA2, Phospholipase A2 Group VII, platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) acetyl hydrolase (PAFAH), PAF-AH.

Size :

25 μg

Price (USD) :

375 USD

Reactivity :

Human

Host :

293E cells

Conjugate/Tag/Label :

RECOM

Application Notes :

Human PLA2G7/Lp-PLA2 Activity Assay Human PLA2G7 activity is measured by its ability to cleave a chromogenic peptide substrate 2-Thio-PAF. The progress of the product formation is monitored by increase in intensity of absorbance at 405 nm in the presence of DTNB. Materials and Buffers 1. Assay Buffer: pH 7.0, 25 mM Sodium Phosphate, 150 mM NaCl, 0.1% BSA (w/v) 2. Human PLA2G7 3. PLA2G7 substrate: 2-Thio-PAF (1-O-hexadecyl-2-deoxy-2-thio-S-acetyl-sn-glyceryl-3-phosphorylcholine), 20 mM in DMSO 4. DTNB (5,5 -dithio-bis(2-nitrobenzoic acid)), 20 mM in DMSO Assay Procedures 1. Dilute activated hPLA2G7 to 0.2 ug/mL with Assay Buffer. 2. Dilute the substrate and DTNB together at 200 uM each in Assay Buffer. 3. Load 50 uL of the diluted hPLA2G7 solution into a plate and 50 uL of assay buffer for the substrate blank, and start a reaction by adding 50 uL of 200 uM of the substrate stock solution into each well. 4. Read the reaction progress by monitoring absorbance at 405 nm in kinetic mode for five minutes. 5. The final human hPLA2G7 concentration is at 0.1 ug/mL (0.01 ug), the substrate concentration at 100 uM, and DTNB at 100 uM. Conversion factor at 405 nm is 23.5 pmol/mAu by using extinction coefficient 13260 M -1 cm -1 and 0.32 cm of light path correction at total volume of 100 uL. . BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee . If you have any concerns, contact us at tech@biolegend.com .