Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Human PLA2G7 (carrier-free)

762902

10 μg

223 USD

BA

762902

Recombinant Human PLA2G7 (carrier-free)

Lipoprotein binding phospholipase A2 (Lp-PLA2), LDL-PLA2, Phospholipase A2 Group VII, platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) acetyl hydrolase (PAFAH), PAF-AH.

10 μg

223 USD

Human

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RECOM

Human PLA2G7/Lp-PLA2 Activity AssayHuman PLA2G7 activity is measured by its ability to cleave a chromogenic peptide substrate 2-Thio-PAF. The progress of the product formation is monitored by increase in intensity of absorbance at 405 nm in the presence of DTNB.Materials and Buffers1. Assay Buffer: pH 7.0, 25 mM Sodium Phosphate, 150 mM NaCl, 0.1% BSA (w/v)2. Human PLA2G73. PLA2G7 substrate: 2-Thio-PAF (1-O-hexadecyl-2-deoxy-2-thio-S-acetyl-sn-glyceryl-3-phosphorylcholine), 20 mM in DMSO4. DTNB (5,5'-dithio-bis(2-nitrobenzoic acid)), 20 mM in DMSOAssay Procedures1. Dilute activated hPLA2G7 to 0.2 µg/mL with Assay Buffer.2. Dilute the substrate and DTNB together at 200 µM each in Assay Buffer.3. Load 50 µL of the diluted hPLA2G7 solution into a plate and 50 µL of assay buffer for the substrate blank, and start a reaction by adding 50 µL of 200 µM of the substrate stock solution into each well.4. Read the reaction progress by monitoring absorbance at 405 nm in kinetic mode for five minutes.5. The final human hPLA2G7 concentration is at 0.1 µg/mL (0.01 µg), the substrate concentration at 100 µM, and DTNB at 100 µM.Conversion factor at 405 nm is 23.5 pmol/mAu by using extinction coefficient 13260 M^-1cm^-1 and 0.32 cm of light path correction at total volume of 100 µL.;BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;