Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Human MMP-7 (carrier-free)

761306

100 μg

698 USD

BA

761306

Recombinant Human MMP-7 (carrier-free)

Matrilysin, Matrix Metalloproteinase 7, MMP7, MMP

100 μg

698 USD

Human

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RECOM

Human MMP-7 Activity Assay Human MMP-7 (hMMP-7) activity is measured by its ability to cleave a fluorogenic peptide substrate Mca-PLGL(Dpa)AR after its activation in the presence of p-Aminophenylmercuric acid. The increase of the product is monitored by increase in intensity of fluorescence at 405 nm with excitation at 320 nm. Materials;Assay Buffer: TCNB (50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35, pH 7.5);Recombinant Human MMP-7;Substrate: MCa-PLGL(Dpa)AR;4-Aminophenylmercuric acetate (APMA);Activity Assay Procedures;Dilute hMMP-7 in the assay buffer at 50 µg/mL.;Activate hMMP-7 by adding APMA to a final concentration of 1 mM.;Incubate the activating mixture for 30 minutes at 37°C.;Dilute the activated hMMP-7 to 0.4 ug/mL (0.4 ng/µL) in Assay Buffer.;Dilute the substrate at 20 µM in Assay Buffer.;Load into a well plate 50 μL of the 0.4 ng/μL hMM-7 and start the reaction by adding 50 μL of 20 μM Substrate. Include a substrate blank containing 50 μL Assay Buffer and 50 μL of 20 μM Substrate without any hMMP7.;Read the product formation by measuring 320/405 nm (Excitation/Emission) in kinetic mode for 5 minutes.;The final hMMP-7 concentration is 0.2 µg/mL (200 ng/ml, 20 ng) and the substrate is 10 µM.; This protein is in the latent form and needs to be activated for bioassay.;BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;