Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Mouse ACE2 (carrier-free)

720102

10 μg

229 USD

BA

720102

Recombinant Mouse ACE2 (carrier-free)

Angiotensin I Converting Enzyme 2, Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 2, Angiotensin-Converting Enzyme Homolog, ACE-Related Carboxypeptidase, Peptidyl-Dipeptidase A, ACEH, ACE-2

10 μg

229 USD

Mouse

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RECOM

Mouse ACE2 Enzymatic Assay Mouse angiotensin I-converting enzyme 2 (ACE2) cleaves the fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH. The increase of the product is monitored by an increase in intensity of fluorescence at 405 nm with excitation at 320 nm. The specific activity is > 400 pmol/min/µg in the presence of 0.025 µg of recombinant mouse ACE2. Materials;Assay buffer: 75 mM Tris, 1 M NaCl, pH 7.5;Recombinant mouse ACE2;Substrate: MCA-Tyr-Val-Ala-Asp-Ala-Pro-Lys(DNP)-OH, 10 mM stock in DMSO (Enzo Life Sciences, Cat. No. ALX-260-023-M001 or equivalent);F16 Black Maxisorp plate (Nunc, Cat. No. 475515);Fluorescent plate reader (Model: SpectraMax M3 by Molecular Devices) or equivalent;Procedure;Dilute the recombinant mouse ACE2 to 0.5 ng/µL in assay buffer.;Dilute the substrate to 20 µM in assay buffer.;Load in a black well plate 50 µL of 0.5 ng/µL of recombinant mouse ACE2, and start the reaction by adding 50 µL of 20 µM substrate. As a control, load 50 µL of 20 µM substrate with 50 µL of assay buffer.;Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 minutes.;Calculate specific activity: Specific Activity (pmol/min/µg) = Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) amount of enzyme (µg);*Adjusted for Substrate Blank**Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog No. M-1975)Per Well:Recombinant mouse ACE2: 0.025 µgSubstrate: 10 µMBioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;