Babco, Signet, Sternberger Monoclonals, Senetek, Covance

protein

Recombinant Human Furin (carrier-free)

719402

10 μg

218 USD

BA

719402

Recombinant Human Furin (carrier-free)

FUR, PACE, PCSK3, SPC1

10 μg

218 USD

Human

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RECOM

Human Furin activity is measured by its ability to cleave a fluorogenic peptide substrate pERTKR-AMC. The increase of the product is monitored by increase in intensity of fluorescence at 460 nm with excitation at 380 nm.; Materials;Assay Buffer: pH 9.0, 25 mM Tris, 1 mM CaCl2, 0.5% Brij-35;Recombinant Human Furin;Furin Substrate: pERTKR-AMC; Activity assay procedures;Dilute hFurin in the assay buffer at 4 µg/mL.;Dilute the substrate at 100 µM in Assay Buffer.;Load into a well plate 50 μL of the 4 ng/μL hFurin and start the reaction by adding 50 μL of 100 μM Substrate. Include a substrate blank containing 50 μL of Assay Buffer and 50 μL of 100 μM Substrate solution.;Read the product formation by measuring 380/460 nm (Excitation/Emission) in kinetic mode for 5 minutes.; The final hFurin concentration is 2 µg/mL (200 ng) and the substrate is 50 µM.;BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.;