product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 594 anti-RNA Polymerase II RPB1
catalog :
664908
quantity :
100 μg
price :
405 USD
clonality :
monoclonal
host :
mouse
conjugate :
Alexa Fluor 594
clone name :
8WG16
reactivity :
fission yeast, Saccharomycetales, human, mouse, brewer's yeast
application :
western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
more info or order :
citations: 33
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:200; loading ...; fig 5a
  • western blot; mouse; 1:500; loading ...; fig 3f
Hartenian E, Gilbertson S, Federspiel J, Cristea I, Glaunsinger B. RNA decay during gammaherpesvirus infection reduces RNA polymerase II occupancy of host promoters but spares viral promoters. PLoS Pathog. 2020;16:e1008269 pubmed publisher
  • western blot; mouse; loading ...; fig 1g
Nishioka K, Miyazaki H, Soejima H. Unbiased shRNA screening, using a combination of FACS and high-throughput sequencing, enables identification of novel modifiers of Polycomb silencing. Sci Rep. 2018;8:12128 pubmed publisher
  • western blot; brewer's yeast; fig 1c
Sdano M, Fulcher J, Palani S, Chandrasekharan M, Parnell T, Whitby F, et al. A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription. elife. 2017;6: pubmed publisher
  • chromatin immunoprecipitation; Saccharomycetales
Rosado Lugo J, Hampsey M. The Ssu72 phosphatase mediates the RNA polymerase II initiation-elongation transition. J Biol Chem. 2014;289:33916-26 pubmed publisher
  • chromatin immunoprecipitation; human
Moquet Torcy G, Tolza C, Piechaczyk M, Jariel Encontre I. Transcriptional complexity and roles of Fra-1/AP-1 at the uPA/Plau locus in aggressive breast cancer. Nucleic Acids Res. 2014;42:11011-24 pubmed publisher
  • chromatin immunoprecipitation; Saccharomycetales
  • immunoprecipitation; Saccharomycetales
  • chromatin immunoprecipitation; human
Mirón García M, Garrido Godino A, Martínez Fernández V, Fernández Pévida A, Cuevas Bermúdez A, Martín Expósito M, et al. The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription. Nucleic Acids Res. 2014;42:9666-76 pubmed publisher
  • western blot; brewer's yeast
Gaillard H, Aguilera A. Cleavage factor I links transcription termination to DNA damage response and genome integrity maintenance in Saccharomyces cerevisiae. PLoS Genet. 2014;10:e1004203 pubmed publisher
Gyenis A, Chang J, Demmers J, Bruens S, Barnhoorn S, Brandt R, et al. Genome-wide RNA polymerase stalling shapes the transcriptome during aging. Nat Genet. 2023;55:268-279 pubmed publisher
Hassan A, Kafai N, Dmitriev I, Fox J, Smith B, Harvey I, et al. A Single-Dose Intranasal ChAd Vaccine Protects Upper and Lower Respiratory Tracts against SARS-CoV-2. Cell. 2020;183:169-184.e13 pubmed publisher
Ge C, Monk I, Pizzolla A, Wang N, Bedford J, Stinear T, et al. Bystander Activation of Pulmonary Trm Cells Attenuates the Severity of Bacterial Pneumonia by Enhancing Neutrophil Recruitment. Cell Rep. 2019;29:4236-4244.e3 pubmed publisher
Wu S, Naar A. SREBP1-dependent de novo fatty acid synthesis gene expression is elevated in malignant melanoma and represents a cellular survival trait. Sci Rep. 2019;9:10369 pubmed publisher
Wu T, Shi L, Lowe A, Nicolls M, Kao P. Inducible expression of immediate early genes is regulated through dynamic chromatin association by NF45/ILF2 and NF90/NF110/ILF3. PLoS ONE. 2019;14:e0216042 pubmed publisher
Chi B, O Connell J, Iocolano A, Coady J, Yu Y, Gangopadhyay J, et al. The neurodegenerative diseases ALS and SMA are linked at the molecular level via the ASC-1 complex. Nucleic Acids Res. 2018;46:11939-11951 pubmed publisher
Krause M, Love D, Ghosh S, Wang P, Yun S, Fukushige T, et al. Nutrient-Driven O-GlcNAcylation at Promoters Impacts Genome-Wide RNA Pol II Distribution. Front Endocrinol (Lausanne). 2018;9:521 pubmed publisher
Akhrymuk I, Frolov I, Frolova E. Sindbis Virus Infection Causes Cell Death by nsP2-Induced Transcriptional Shutoff or by nsP3-Dependent Translational Shutoff. J Virol. 2018;92: pubmed publisher
Kamada R, Yang W, Zhang Y, Patel M, Yang Y, Ouda R, et al. Interferon stimulation creates chromatin marks and establishes transcriptional memory. Proc Natl Acad Sci U S A. 2018;115:E9162-E9171 pubmed publisher
Fuentes D, Swigut T, Wysocka J. Systematic perturbation of retroviral LTRs reveals widespread long-range effects on human gene regulation. elife. 2018;7: pubmed publisher
Minocha R, Popova V, Kopytova D, Misiak D, Huttelmaier S, Georgieva S, et al. Mud2 functions in transcription by recruiting the Prp19 and TREX complexes to transcribed genes. Nucleic Acids Res. 2018;46:9749-9763 pubmed publisher
Yu R, Wang X, Moazed D. Epigenetic inheritance mediated by coupling of RNAi and histone H3K9 methylation. Nature. 2018;558:615-619 pubmed publisher
Gouot E, Bhat W, Rufiange A, Fournier E, Paquet E, Nourani A. Casein kinase 2 mediated phosphorylation of Spt6 modulates histone dynamics and regulates spurious transcription. Nucleic Acids Res. 2018;46:7612-7630 pubmed publisher
Li S, Almeida A, Radebaugh C, Zhang L, Chen X, Huang L, et al. The elongation factor Spn1 is a multi-functional chromatin binding protein. Nucleic Acids Res. 2018;46:2321-2334 pubmed publisher
Li P, Mitra S, Spolski R, Oh J, Liao W, Tang Z, et al. STAT5-mediated chromatin interactions in superenhancers activate IL-2 highly inducible genes: Functional dissection of the Il2ra gene locus. Proc Natl Acad Sci U S A. 2017;114:12111-12119 pubmed publisher
García Benítez F, Gaillard H, Aguilera A. Physical proximity of chromatin to nuclear pores prevents harmful R loop accumulation contributing to maintain genome stability. Proc Natl Acad Sci U S A. 2017;114:10942-10947 pubmed publisher
Jih G, Iglesias N, Currie M, Bhanu N, Paulo J, Gygi S, et al. Unique roles for histone H3K9me states in RNAi and heritable silencing of transcription. Nature. 2017;547:463-467 pubmed publisher
Sood V, Cajigas I, D Urso A, Light W, Brickner J. Epigenetic Transcriptional Memory of GAL Genes Depends on Growth in Glucose and the Tup1 Transcription Factor in Saccharomyces cerevisiae. Genetics. 2017;206:1895-1907 pubmed publisher
Dorighi K, Swigut T, Henriques T, Bhanu N, Scruggs B, Nady N, et al. Mll3 and Mll4 Facilitate Enhancer RNA Synthesis and Transcription from Promoters Independently of H3K4 Monomethylation. Mol Cell. 2017;66:568-576.e4 pubmed publisher
Crickard J, Lee J, Lee T, Reese J. The elongation factor Spt4/5 regulates RNA polymerase II transcription through the nucleosome. Nucleic Acids Res. 2017;45:6362-6374 pubmed publisher
Klopf E, Schmidt H, Clauder Münster S, Steinmetz L, Schüller C. INO80 represses osmostress induced gene expression by resetting promoter proximal nucleosomes. Nucleic Acids Res. 2017;45:3752-3766 pubmed publisher
Garavís M, González Polo N, Allepuz Fuster P, Louro J, Fernández Tornero C, Calvo O. Sub1 contacts the RNA polymerase II stalk to modulate mRNA synthesis. Nucleic Acids Res. 2017;45:2458-2471 pubmed publisher
Ahn J, Rechsteiner A, Strome S, Kelly W. A Conserved Nuclear Cyclophilin Is Required for Both RNA Polymerase II Elongation and Co-transcriptional Splicing in Caenorhabditis elegans. PLoS Genet. 2016;12:e1006227 pubmed publisher
Tsai S, Chang Y, Swamy K, Chiang R, Huang D. GAGA factor, a positive regulator of global gene expression, modulates transcriptional pausing and organization of upstream nucleosomes. Epigenetics Chromatin. 2016;9:32 pubmed publisher
Shi L, Godfrey W, Lin J, Zhao G, Kao P. NF90 regulates inducible IL-2 gene expression in T cells. J Exp Med. 2007;204:971-7 pubmed
Funayama R, Saito M, Tanobe H, Ishikawa F. Loss of linker histone H1 in cellular senescence. J Cell Biol. 2006;175:869-80 pubmed
product information
Antigen :
RNA Polymerase II RPB1
Apps. Abbrev. :
ICC
Cat # :
664908
Clone :
8WG16
Item :
Alexa Fluor® 594 anti-RNA Polymerase II RPB1
Isotype :
Mouse IgG2a
Other Names :
RNA polymerase II sub-unit 1, RPB1, CTD repeat YSPTSPS, C- terminal domain repeat YSPTSPS, DNA-directed RNA polymerase II subunit A, RNA-directed RNA polymerase II subunit RPB1, POLR2A, POLR2
Size :
100 μg
Price (USD) :
405 USD
Reactivity :
Human, Mouse
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A594
Immunogen :
Wheat germ RNA Polymerase II
Application Notes :
Clone 8WG16 recognizes YSPTSPS in the C-terminal domain. The antibody cross-reacts with wheat, yeast, mouse, c. elegans, x. laevis, and most eukaryotic RNAPII.Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation, functional assay, and immunoprecipatation.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA