Anti-RNA Polymerase II RPB1 | BioLegend 664903 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Anti-RNA Polymerase II RPB1

catalog :

664903

quantity :

100 μl

price :

275 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8WG16

reactivity :

fission yeast, budding yeasts, human, mouse, rat, baker's yeast, Caenorhabditis elegans

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 29

Published Application/Species/Dilution	Reference
chromatin immunoprecipitation; baker's yeast; fig 5a	Klopf E, Schmidt H, Clauder-Münster S, Steinmetz L, Schüller C. INO80 represses osmostress induced gene expression by resetting promoter proximal nucleosomes. Nucleic Acids Res. 2016;: pubmed publisher
western blot; human; fig 3	Steingruber M, Kraut A, Socher E, Sticht H, Reichel A, Stamminger T, et al. Proteomic Interaction Patterns between Human Cyclins, the Cyclin-Dependent Kinase Ortholog pUL97 and Additional Cytomegalovirus Proteins. Viruses. 2016;8: pubmed publisher
western blot; baker's yeast; fig 3c	Garavís M, González-Polo N, Allepuz-Fuster P, Louro J, Fernández-Tornero C, Calvo O. Sub1 contacts the RNA polymerase II stalk to modulate mRNA synthesis. Nucleic Acids Res. 2016;: pubmed
chromatin immunoprecipitation; mouse; fig 1d	Bailus B, Pyles B, McAlister M, O'Geen H, Lockwood S, Adams A, et al. Protein Delivery of an Artificial Transcription Factor Restores Widespread Ube3a Expression in an Angelman Syndrome Mouse Brain. Mol Ther. 2016;24:548-55 pubmed publisher
ChIP-Seq; human; fig 2	Rialdi A, Campisi L, Zhao N, Lagda A, Pietzsch C, Ho J, et al. Topoisomerase 1 inhibition suppresses inflammatory genes and protects from death by inflammation. Science. 2016;352:aad7993 pubmed publisher
western blot; mouse; fig 2	Ide S, Déjardin J. End-targeting proteomics of isolated chromatin segments of a mammalian ribosomal RNA gene promoter. Nat Commun. 2015;6:6674 pubmed publisher
ChIP-Seq; mouse; fig 2 immunoprecipitation; mouse; fig s5	Masuda A, Takeda J, Okuno T, Okamoto T, Ohkawara B, Ito M, et al. Position-specific binding of FUS to nascent RNA regulates mRNA length. Genes Dev. 2015;29:1045-57 pubmed publisher
chromatin immunoprecipitation; human	Stubbs S, Conrad N. Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy. Nucleic Acids Res. 2015;43:504-19 pubmed publisher
western blot; human; fig 2	Liang K, Gao X, Gilmore J, Florens L, Washburn M, Smith E, et al. Characterization of human cyclin-dependent kinase 12 (CDK12) and CDK13 complexes in C-terminal domain phosphorylation, gene transcription, and RNA processing. Mol Cell Biol. 2015;35:928-38 pubmed publisher
ChIP-Seq; human; fig 2	Mannini L, C Lamaze F, Cucco F, Amato C, Quarantotti V, Rizzo I, et al. Mutant cohesin affects RNA polymerase II regulation in Cornelia de Lange syndrome. Sci Rep. 2015;5:16803 pubmed publisher
western blot; baker's yeast	Gaillard H, Aguilera A. Cleavage factor I links transcription termination to DNA damage response and genome integrity maintenance in Saccharomyces cerevisiae. PLoS Genet. 2014;10:e1004203 pubmed publisher
western blot; human	Lodhi N, Kossenkov A, Tulin A. Bookmarking promoters in mitotic chromatin: poly(ADP-ribose)polymerase-1 as an epigenetic mark. Nucleic Acids Res. 2014;42:7028-38 pubmed publisher
chromatin immunoprecipitation; human	Moquet-Torcy G, Tolza C, Piechaczyk M, Jariel-Encontre I. Transcriptional complexity and roles of Fra-1/AP-1 at the uPA/Plau locus in aggressive breast cancer. Nucleic Acids Res. 2014;42:11011-24 pubmed publisher
chromatin immunoprecipitation; budding yeasts chromatin immunoprecipitation; human immunoprecipitation; budding yeasts	Mirón-García M, Garrido-Godino A, Martínez-Fernández V, Fernández-Pevida A, Cuevas-Bermúdez A, Martín-Expósito M, et al. The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription. Nucleic Acids Res. 2014;42:9666-76 pubmed publisher
western blot; human; fig 2	Lama L, Seidl C, Ryan K. New insights into the promoterless transcription of DNA coligo templates by RNA polymerase III. Transcription. 2014;5:e27913 pubmed publisher
chromatin immunoprecipitation; budding yeasts	Rosado-Lugo J, Hampsey M. The Ssu72 phosphatase mediates the RNA polymerase II initiation-elongation transition. J Biol Chem. 2014;289:33916-26 pubmed publisher
chromatin immunoprecipitation; mouse; 5 ug/time	Konsavage W, Yochum G. The myc 3' wnt-responsive element suppresses colonic tumorigenesis. Mol Cell Biol. 2014;34:1659-69 pubmed publisher
western blot; fission yeast	Karakasili E, Burkert-Kautzsch C, Kieser A, Sträßer K. Degradation of DNA damage-independently stalled RNA polymerase II is independent of the E3 ligase Elc1. Nucleic Acids Res. 2014;42:10503-15 pubmed publisher
chromatin immunoprecipitation; rat	DiNieri J, Wang X, Szutorisz H, Spano S, Kaur J, CASACCIA P, et al. Maternal cannabis use alters ventral striatal dopamine D2 gene regulation in the offspring. Biol Psychiatry. 2011;70:763-9 pubmed publisher
chromatin immunoprecipitation; fission yeast	DeGennaro C, Alver B, Marguerat S, Stepanova E, Davis C, Bahler J, et al. Spt6 regulates intragenic and antisense transcription, nucleosome positioning, and histone modifications genome-wide in fission yeast. Mol Cell Biol. 2013;33:4779-92 pubmed publisher
western blot; human; 1:1,000	Medina R, Ghule P, Cruzat F, Barutcu A, Montecino M, Stein J, et al. Epigenetic control of cell cycle-dependent histone gene expression is a principal component of the abbreviated pluripotent cell cycle. Mol Cell Biol. 2012;32:3860-71 pubmed publisher
chromatin immunoprecipitation; human	Kuwahara Y, Wei D, Durand J, Weissman B. SNF5 reexpression in malignant rhabdoid tumors regulates transcription of target genes by recruitment of SWI/SNF complexes and RNAPII to the transcription start site of their promoters. Mol Cancer Res. 2013;11:251-60 pubmed publisher
	Ahn J, Rechsteiner A, Strome S, Kelly W. A Conserved Nuclear Cyclophilin Is Required for Both RNA Polymerase II Elongation and Co-transcriptional Splicing in Caenorhabditis elegans. PLoS Genet. 2016;12:e1006227 pubmed publisher
	Dias J, Rito T, Torlai Triglia E, Kukalev A, Ferrai C, Chotalia M, et al. Methylation of RNA polymerase II non-consensus Lysine residues marks early transcription in mammalian cells. elife. 2015;4: pubmed publisher
	Mbogning J, Page V, Burston J, Schwenger E, Fisher R, Schwer B, et al. Functional interaction of Rpb1 and Spt5 C-terminal domains in co-transcriptional histone modification. Nucleic Acids Res. 2015;43:9766-75 pubmed publisher
	Mas G, de Nadal E, Dechant R, Rodríguez de la Concepción M, Logie C, Jimeno-González S, et al. Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genes. EMBO J. 2009;28:326-36 pubmed publisher
	Lassot I, Estrabaud E, Emiliani S, Benkirane M, Benarous R, Margottin-Goguet F. p300 modulates ATF4 stability and transcriptional activity independently of its acetyltransferase domain. J Biol Chem. 2005;280:41537-45 pubmed
	Ramamoorthi K, Fropf R, Belfort G, Fitzmaurice H, McKinney R, Neve R, et al. Npas4 regulates a transcriptional program in CA3 required for contextual memory formation. Science. 2011;334:1669-75 pubmed publisher
	Clark E, Hadjimichael C, Temperley R, Barnard A, Fuller-Pace F, Robson C. p68/DdX5 supports β-catenin & RNAP II during androgen receptor mediated transcription in prostate cancer. PLoS ONE. 2013;8:e54150 pubmed publisher

product information

Antigen :

RNA Polymerase II RPB1

Apps. Abbrev. :

Western Blotting, ChIP, FA, IP

Cat # :

664903

Clone :

8WG16

Item :

Anti-RNA Polymerase II RPB1

Isotype :

Mouse IgG2a

Other Names :

RNA polymerase II sub-unit 1, RPB1, CTD repeat YSPTSPS, C- terminal domain repeat YSPTSPS, DNA-directed RNA polymerase II subunit A, RNA-directed RNA polymerase II subunit RPB1, POLR2A, POLR2, W0011

Size :

100 μl

Price (USD) :

275 USD

Reactivity :

Human, Yeast, Wheat, Mouse, C. elegans, X. laevis

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

Ascites

Immunogen :

Wheat germ RNA Polymerase II

Application Notes :

Clone 8WG16 recognizes YSPTSPS in the C-terminal domain. The antibody cross-reacts with wheat, yeast, mouse, c. elegans, x. laevis , and most eukaryotic RNAPII. Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation, functional assay, and immunoprecipatation.