Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

PE anti-NFATc1

649606

100 μg

360 USD

monoclonal

mouse

PE

7A6

human, mouse, rat

ICFC

649606

7A6

PE anti-NFATc1

Mouse IgG1, κ

Nuclear factor of activated T-cells, cytoplasmic 1, NFAT transcription complex cytosolic component, NFAT2, NFATc

100 μg

360 USD

Human, Mouse, Rat

Monoclonal

Mouse

PE

Recombinant protein of human NFATc1 amino acids 197-304.

7A6 antibody detects endogenous human NFATC1 in Western blot with an approximate molecular weight of 100 - 120 kD (different isoforms) (Cat. No. 649602). The optimal concentration should be determined by titration for each individual assay of interest.; Clone 7A6 Intracellular Staining Procedure: ;Prepare the fix/perm solution, consisting of 1% paraformaldehyde in 70% ethanol. Chill for at least one hour at -20°C.;Suspend cells in cell staining buffer and perform surface stain if necessary.;Centrifuge for 5 minutes at 1500 RPM and discard supernatant.;Resuspend cells in 500 µL of fix/perm solution for up to 2 x 10^6 cells and gently vortex.;Incubate cells on ice for 30-60 minutes in the dark.;Add 2 mL PBS and centrifuge cells for 1500 RPM for 5 minutes.;Wash cells 1X with 2 mL cell staining buffer, centrifuge at 1500 RPM for 5 minutes, discard supernatant.;Adjust cell concentration to about 1 x 10^7 cells/mL, add 100 µL (about 1 x 10^6 cells) to a FACS tube that already contains the staining antibody and mix gently.;Incubate 15 minutes at room temperature.;Wash 2X with cell staining buffer as in step 6.;Resuspend cells in cell staining buffer and analyze.;