PE anti-NFATc1 | BioLegend 649605 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE anti-NFATc1

catalog :

649605

quantity :

25 μg

price :

153 USD

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

7A6

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

BioLegend product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human immunohistochemistry; human	Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed publisher
	Wijesinghe A, Gamage J, Goonewardena H, Gomes L, Jayathilaka D, Wijeratne D, et al. Phenotype and functionality of follicular helper T cells in patients with acute dengue infection. J Biomed Sci. 2020;27:50 pubmed publisher
	Fan W, Liang D, Tang Y, Qu B, Cui H, Luo X, et al. Identification of microRNA-31 as a novel regulator contributing to impaired interleukin-2 production in T cells from patients with systemic lupus erythematosus. Arthritis Rheum. 2012;64:3715-25 pubmed publisher

product information

Antigen :

NFATc1

Apps. Abbrev. :

ICFC

Cat # :

649605

Clone :

7A6

Item :

PE anti-NFATc1

Isotype :

Mouse IgG1, κ

Other Names :

Nuclear factor of activated T-cells, cytoplasmic 1, NFAT transcription complex cytosolic component, NFAT2, NFATc

Size :

25 μg

Price (USD) :

153 USD

Reactivity :

Human, Mouse, Rat

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

Immunogen :

Recombinant protein of human NFATc1 amino acids 197-304.

Application Notes :

7A6 antibody detects endogenous human NFATC1 in Western blot with an approximate molecular weight of 100 - 120 kD (different isoforms) (Cat. No. 649602). The optimal concentration should be determined by titration for each individual assay of interest.; Clone 7A6 Intracellular Staining Procedure: ;Prepare the fix/perm solution, consisting of 1% paraformaldehyde in 70% ethanol. Chill for at least one hour at -20°C.;Suspend cells in cell staining buffer and perform surface stain if necessary.;Centrifuge for 5 minutes at 1500 RPM and discard supernatant.;Resuspend cells in 500 µL of fix/perm solution for up to 2 x 10^6 cells and gently vortex.;Incubate cells on ice for 30-60 minutes in the dark.;Add 2 mL PBS and centrifuge cells for 1500 RPM for 5 minutes.;Wash cells 1X with 2 mL cell staining buffer, centrifuge at 1500 RPM for 5 minutes, discard supernatant.;Adjust cell concentration to about 1 x 10^7 cells/mL, add 100 µL (about 1 x 10^6 cells) to a FACS tube that already contains the staining antibody and mix gently.;Incubate 15 minutes at room temperature.;Wash 2X with cell staining buffer as in step 6.;Resuspend cells in cell staining buffer and analyze.;

more info or order :

BioLegend product webpage